Effect of season and stage of lactation on milk components in Purnathadi buffaloes

Kuralkar, Prajakta; Kuralkar, Shailendra

doi:10.56825/bufbu.2022.4133165

Cited by 5 publications

(6 citation statements)

References 5 publications

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“…Our results are consistent with this view, as glu levels reached maximum values when the load was 500 kg. The glu values observed in the present study are consistent with those in adult buffaloes previously reported by Mahardika et al [9], Mahardika et al [41], and Kuralkar et al [50]. In general, glu concentrations above reference values can be attributed to the activation of the hepatic processes of glycogenolysis and gluconeogenesis, which is a response to the energetic need to maintain muscle activity.…”

Section: Discussionsupporting

confidence: 92%

“…[ 41 ], and Kuralkar et al . [ 50 ]. In general, glu concentrations above reference values can be attributed to the activation of the hepatic processes of glycogenolysis and gluconeogenesis, which is a response to the energetic need to maintain muscle activity.…”

Section: Discussionmentioning

confidence: 99%

“…According to Patel et al . [ 56 ], elevated erythrocyte values in adult buffalo are due to the action of androgens in enhancing the growth of erythroid progenitor cells in the presence of erythropoietin, leading to a higher rate of erythropoiesis, which may also be related to the levels of Hb and HCT [ 50 ]. Likewise, the higher erythrocyte count in adult buffalo may be due to a high basal metabolic rate, leading to a higher rate of erythropoiesis that raises the erythrocyte count [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of different load weights on the work performance and physiological and hematobiochemical responses in working water buffalo

Paján-Jiménez,

Pazmiño-Rodríguez,

Roldán-Santiago

et al. 2023

Vet World

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Background and Aim: Working animals are important in agriculture because they play a role in various agricultural activities, including milk and meat production. Thus, they contribute to the development of rural communities. In this study, we aimed to evaluate the effects of different load weights on the work performance and the physiological and hematological responses of working water buffalo (Bubalus bubalis). Materials and Methods: The work performances of 12 buffaloes (average weight 782.16 ± 21.62 kg) transporting 200, 350, and 500 kg of African palm fruits in metal baskets placed on their backs were evaluated. Work performance variables evaluated immediately after work were as follows: total number of trips (n), total weight (kg), distance traveled (km/day), working period (h), stopped time (h), and average speed (km/h). In addition, we evaluated physiological, biochemical, and hematological variables at three different times: before the start of work, immediately after work, and on the rest day. Results: Among the load weights, 500 kg (total load carried = 4,138.88 kg) improved work efficiency compared to 200 kg loads (total load = 3,322.22 kg) (p = 0.0281). However, 500 kg loads resulted in slower average speed (2.4 km, p = 0.0164), shorter working period (2.39 h, p < 0.0001) and distance traveled (7.29 km, p < 0.0001), and less total number of trips (8.27 trips, p < 0.0001) compared to 350 and 200 kg load weights (3.45 and 3.52 km/h, 2.55 and 2.79 h, 8.71 and 9.75 km, 10.94 and 16.61 trips, respectively); and the heaviest loads resulted in significantly higher (p < 0.005) respiratory rate, pulse, heart rate, rectal temperature, glucose, lactate dehydrogenase, creatine kinase, total protein, white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In contrast, was associated with lower levels of red blood cells, hemoglobin, and hematocrit compared to lower loads. All differences were more pronounced in 500 kg (p < 0.005) compared to 200 kg loads. Conclusion: Working buffaloes responded to work related to the transport of African palm fruits through various physiological, biochemical, and hematological changes. However, some variables remained close to the reference values reported in the literature for water buffaloes, and in general, all variables were reestablished during the rest day, indicating that these animals have adapted to working conditions. Keywords: biochemical responses, draught buffalo, hematological responses, physiological responses.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of different load weights on the work performance and physiological and hematobiochemical responses in working water buffalo

Paján-Jiménez,

Pazmiño-Rodríguez,

Roldán-Santiago

et al. 2023

Vet World

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“…This method offers a convenient assay of large numbers of samples with excellent precision. The practice of RIA in the measurement of hormones for veterinary clinical use has been extensively studied in India (Dadke et al, 2018;Roopali et al, 2020;Galdhar et al, 2021;Jayabhaye et al, 2021;Galdhar et al, 2022 andSalutgi et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Studies on the Assessment of Hair Cortisol Concentration in Dogs by Radioimmunoassay (RIA)

Galdhar,

Vyas,

Gaikwad

et al. 2024

IJAR

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Background: Hair cortisol Concentration (HCC) is often used as a measure of chronic stress responses in humans. The use of Radioimmunoassay (RIA) and HCC to assess stress in hospitalized dogs has been poorly reported in the literature in India and Central East Asia. This paper presents a modified protocol for measuring HCC in dog hair samples for stress assessment using RIA. Methods: Estimation of HCC concentrations of healthy (n=20) and hospitalized (n=20) dogs was done using RIA. Hair samples were collected from a dog’s nape region and weighed to measure HCC concentration. The study used RIA-enabled dissevered spiking and detectable concentration-based modifications to measure HCC. Five known cortisol concentration standards (viz 19.0 nmol/l, 47.9 nmol/l, 190 nmol/l, 238.66 nmol/l and 713.33 nmol/l) were used, with the first three readily available with a cortisol RIA kit. The samples were spiked with 50 µL of the standard and analysed in a gamma counter to take counts per minute (CPM). The final concentration of HCC was estimated in nmol/l and derived in pg/mg as per standard methods. Result: Healthy dogs had a mean HCC of 6.24±0.84 pg/mg, while hospitalized dogs had 31.65±5.87 pg/mg. A significant deviation was observed in both groups. A box and whisker graph showed an HCC range of 0.36 to 13.78 pg/mg for healthy dogs and 8.70 to 65.25 pg/mg for hospitalized dogs. The interquartile range (25th to 75th percentile) was 3.55 to 9.24 pg/mg for healthy dogs and 14.43 to 46.22 for hospitalized dogs. The observed median for healthy dogs was 6.16 pg/mg and 22.62 pg/mg for hospitalized dogs. The study shows higher HCC in hospitalized sick dogs compared to clinically healthy ones, suggesting hair sample collection as a simple stress assessment method and RIA as a promising HCC detection technique.

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“…Alterations in thyroid hormone concentrations in response to Non-Thyroid Illness (NTIs) are well recognized in veterinary clinical practice ( 1 , 2 ). In severe NTIs, there is a decreased concentration of Triiodothyronine (TT 3 ) and Total thyroxine (TT 4 ).…”

Section: Introductionmentioning

confidence: 99%

Radio Immune Assay (RIA) Enabled Total Triiodothyronine (TT3) and Total Thyroxine (TT4) in Canine Trypanosomiasis: First Case Report from Maharashtra (India)

Salutgi¹,

Galdhar²,

Sonigra³

et al. 2023

IJPA

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Radio Immune Assay (RIA) is an extremely sensitive in vitro assay technique to measure concentrations of antigen viz. hormones in biological fluids using antibodies. The present study reports the status of total triiodothyronine (TT3) and total thyroxine (TT4) in Trypanosoma evansi infection in a dog, year 2022. An adult, non-descript, male dog was referred to the Department of Veterinary Nuclear Medicine, Mumbai Veterinary College, (MAFSU), Parel, Mumbai (India) with a history of inappetence, weakness, and ataxia of the hind limbs. Inspection revealed cachexia, anemia, bedsores, and mild mucopurulent ocular and nasal discharge. Clinical examination revealed pyrexia, polypnoea and tachycardia. There was an enlargement of popliteal, pre-scapular, and submandibular lymph nodes. The blood smear examination revealed severe infection of extracellular T. evansi. Laboratory investigations showed an altered haemato-biochemical profile. RIA-enabled thyroid hormone profile revealed a reduced concentration of TT3 (0.57 nmol/l) and TT4 (22.52 nmol/l). The present study reports a reduction in the concentration of TT3 and TT4 in a dog suffering from trypanosomiasis. The drop in TT4 concentration was within the normal limit, this could be a cause for the non-appearance of usual clinical symptoms of hypothyroidism in the present case.

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Effect of season and stage of lactation on milk components in Purnathadi buffaloes

Cited by 5 publications

References 5 publications

Effects of different load weights on the work performance and physiological and hematobiochemical responses in working water buffalo

Effects of different load weights on the work performance and physiological and hematobiochemical responses in working water buffalo

Studies on the Assessment of Hair Cortisol Concentration in Dogs by Radioimmunoassay (RIA)

Radio Immune Assay (RIA) Enabled Total Triiodothyronine (TT3) and Total Thyroxine (TT4) in Canine Trypanosomiasis: First Case Report from Maharashtra (India)

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