Effect of seasonal and experimental temperature on de novo synthesis of fatty acids in C. crangon

Abstract

The intensity of in vivo lipogensis was measured and in this purpose, the radioactivity of incorporation of tritium into fatty acids (FAs) in tissues of C. crangon was determined. De novo synthesis of FAs was five times higher in hepatopancreas than in muscle in summer period but not much higher in autumn. The higher FAs synthesis was recorded at 25 °C, both for hepatopancreas and muscle, and the summer was higher than the autumn in the hepatopancreas and in the muscles of the opposite situation was observed. The higher amounts of SFAs in hepatopancreas from autumn, when in experimental conditions the ambient temperature C. crangon changed from 6 °C to the experimental higher temperature. When content of PUFAn-3 declined dramatically (Autumn 1 h, 25 °C). In contrast, at a lower temperature, the amount of polyunsaturated FAs is much higher than at 25 °C (Autumn 1 h 6 °C).

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“…On the other hand, the levels of n-6 PUFA were conserved while n-3 PUFA and LC-PUFA levels were magnified in both the muscle and hepatopacreas during the coldstress. At reduced temperatures, in their study with C. crangon, Mika et al (2014) reported similar response as also did also Perez-Velazquez et al (2003) with L. vannamei. Despite partial starvation coupled with the cold-stress, the significant accumulations of PUFA and LC-PUFA recorded in the tissues of P. semisulcatus might be due to the vital role of these fatty acids in membrane structures of shrimp (Farkas, 1979;Schlechtriem et al, 2006;Werbrouck et al, 2016a).…”

“…Chapelle (1978) acclimated the crab C. maenas to temperatures of 7, 14 and 27°C and observed the greatest differences in the relative content of 16:0, 16:1, 18:0, 18:1, 20:5 and 22:6 fatty acids in relation to temperature. It is suggested by many authors that acclimation temperature involves in the degree of unsaturation of cellular lipids in order to maintain fluidity of cell membranes essential for biological activities (Sinensky, 1974;Dey, Buda, Wiik, Halver, & Farkas, 1993;Perez-Velazquez et al, 2003;Schlechtriem et al, 2006;Mika, Skorkowski, & Stepnowski, 2014).…”

Temporal variation in the fatty acid composition of ovigerous females and embryos of the squat lobsterPleuroncodes monodon(Decapoda, Munididae)

“…Several studies have demonstrated that marine crustaceans (e.g. Crangon crangon : Mika et al ., 2014) and freshwater crustaceans (e.g. Macrobrachium rosenbergii : Reigh & Stickney, 1989) are capable of enzymatically transforming scPUFA into lcPUFA.…”