Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Maia, R. C.; Waltero, Edgar Mauricio Mogollón; Marín, Diego Fernando Dubeibe; Carvalho, B. P.; Dias, Ângelo José Burla

doi:10.17533/udea.rccp.v30n4a01

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…When the cattle were mostly fed in the pasture, the quality of the oocytes was higher and the maturation and postdevelopment rates were better (Maia et al 2017). This result is similar to the high maturation rate we obtained in the spring and the high maturation rate according to the autumn season.…”

Section: Resultssupporting

confidence: 86%

Effect of low doses of FSH and season on the in vitro maturation, fertilization and embryo development of bovine oocytes

̧AHIN

2020

Indian J of Anim Sci

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This study was aimed at determining the effect of follicle-stimulating hormone (FSH) and season on the in vitro maturation, fertilization and embryo development of bovine oocytes. Bovine ovaries obtained from a local slaughter house were transported to the laboratory within 2–3 h in a thermos flask containing antibiotic-supplemented physiological saline (0.9%) and at a fixed temperature of 30°C. Bovine oocytes collected in spring and autumn were incubated in culture media containing FSH at concentrations of 0.2 and 0.8 μg/ml. After maturation, oocytes were fertilized. Fertilized oocytes were incubated in CR1aa culture medium for 7 days at 38.5°C for in vitro development. The assessment made after the completion of the maturation process revealed that, for both FSH doses, the maturation rates obtained with the oocytes collected in spring were higher than those obtained with the oocytes collected in autumn. The incubation of the oocytes collected in autumn in culture media supplemented with 0.2 μg/ml of FSH resulted in a low level of oocyte maturation. After maturation, oocytes were subjected to fertilization. Fertilized oocytes were incubated in CR1aa culture medium for 7 days at 38.5°C for in vitro development. In both seasons, 0.8 μg/ml FSH application was higher than the maturation values obtained with 0.2 μg/ml FSH in terms of fertilization and embryo development rates. The study was repeated 9 times for each season. Although there was no significant difference between fertilizations and embryo development in the seasons, better results were obtained in spring season.

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Section: Resultssupporting

confidence: 86%

Effect of low doses of FSH and season on the in vitro maturation, fertilization and embryo development of bovine oocytes

̧AHIN

2020

Indian J of Anim Sci

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“…Those with unchanged cytoplasm were considered BCB-and less competent. This test is based on the ability of the enzyme glucose-6-phosphate dehydrogenase (G6PDH) to reduce BCB from blue to colorless in growing oocytes, whereas in oocytes grown at low activity the enzyme allows them to remain blue (Maia et al, 2017).…”

Section: Morphological Evaluation and Bright Cresyl Blue Assaymentioning

confidence: 99%

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Fernandes-França¹,

Oliveira-Santos²,

Oliveira-Lira³

et al. 2019

Rev. colomb. cienc. pecu.

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Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.

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Effect of Brilliant cresyl blue (BCB) staining in oocyte’s maturation and parthenogenetic activation in alpaca (Vicugna pacos) oocytes

Vargas-Donayre,

Bravo,

Zeña

et al. 2024

Small Ruminant Research

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Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Cited by 3 publications

References 26 publications

Effect of low doses of FSH and season on the in vitro maturation, fertilization and embryo development of bovine oocytes

Effect of low doses of FSH and season on the in vitro maturation, fertilization and embryo development of bovine oocytes

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Effect of Brilliant cresyl blue (BCB) staining in oocyte’s maturation and parthenogenetic activation in alpaca (Vicugna pacos) oocytes

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