Effect of seaweed elicitation and culture conditions on biomass and metabolite production in in vitro shoots of Withania somnifera

Vinod, Sangeetha; Natarajan, Kanimozhi; Santhanu, Krishnapriya; Selvakumar, Divya; Natesan, Senthil Kumar; Senthil, Kalaiselvi

doi:10.1016/j.sajb.2022.10.051

Cited by 10 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The shoot obtained from the germinated seedlings was used as the source of explants and has been inoculated onto the full-strength MS liquid basal medium, cultured at 25±2 °C and a photoperiod of 16 hours for 30 days. For shoot multiplication, 30 days old shoots were inoculated onto MS medium supplemented with 4.44 µM BAP (6-benzylaminopurine) hormone (Vinod et al, 2022).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Each experiment had 3 replicates with three explants in each. The shoots were harvested after 7 and 14 days of exposure period, weighed, shade dried at room temperature and extracted using methanol (Vinod et al, 2022). The fresh shoot tissues were weighed and the growth index was calculated using the formula:…”

Section: Treatment With Lead Saltsmentioning

confidence: 99%

“…High Performance Thin Layer Chromatography (HPTLC) was performed on precoated silica gel aluminium plate 60F254 (MERCK, Germany) using CAMAG HPTLC for withaferin A quantification. For HPTLC analysis, the procedure reported by Vinod et al (2022) was followed. The concentrated methanol extract was further used for the phytochemical analysis.…”

Section: Quantification Of Secondary Metabolitesmentioning

confidence: 99%

See 2 more Smart Citations

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Natarajan,

Rangasamy,

Santhanu

et al. 2023

View full text Add to dashboard Cite

Withania somnifera is a medicinal ayurvedic plant native to India. Lead (Pb) is a toxic heavy metal which has no biological function in plants. The aim of the present study is to investigate the influence of heavy metal Pb on W. somnifera at cellular level under controlled conditions. The role of Pb on shoots of W. somnifera was determined in terms of biomass, secondary metabolite production (High Performance Thin Layer Chromatography) and phytochemical quantification. Influence and accumulation of Pb at the cellular level were recognized using UV visible spectroscopy, field emission scanning electron microscope and x-ray diffraction analysis. The current result showed that the concentration up to 2.4 mM Pb for 7 days of exposure showed higher biomass and withaferin A yield compared to control shoots. On the other hand, other secondary metabolites such as flavonoids, phytosterols and phenols levels were reduced compared to control shoots. In the morphological study, Pb concentration in the analysed sample was determined as 2%. The probability of the nanoparticle nature of the bioaccumulated lead also verified using spectroscopy and x-ray diffraction analysis. To date, we are the first to report on the influence of Pb on in vitro shoot cultures of W. somnifera.

show abstract

Section: Plant Materialsmentioning

confidence: 99%

Section: Treatment With Lead Saltsmentioning

confidence: 99%

Section: Quantification Of Secondary Metabolitesmentioning

confidence: 99%

See 1 more Smart Citation