Effect of seaweed extracts and plant growth regulators on high-frequency in vitro mass propagation of Lycopersicon esculentum L (tomato) through double cotyledonary nodal explant

Vinoth, S.; Gurusaravanan, P.; Jayabalan, N.

doi:10.1007/s10811-011-9717-9

Cited by 48 publications

(20 citation statements)

References 30 publications

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“…They increase the success of in vitro propagation (Vinoth et al, 2012) and enhance plant protection against pathogens and pests (Hankins and Hockey, 1990;Jayaraman et al, 2011;Khan et al, 2009;Klarzynski et al, 2003;Loureiro et al, 2012;Mercier et al, 2001).…”

Section: Effects Of Se On Plantsmentioning

confidence: 99%

The Use of Biostimulants for Enhancing Nutrient Uptake

Halpern

Bar‐Tal

Ofek

et al. 2015

Advances in Agronomy

386

226

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Section: Effects Of Se On Plantsmentioning

confidence: 99%

The Use of Biostimulants for Enhancing Nutrient Uptake

Halpern

Bar‐Tal

Ofek

et al. 2015

Advances in Agronomy

386

226

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“…As biostimulants and liquid fertilizers, seaweed extracts have been applied as foliar spray and soil drench in organic farming1. Unlike chemical fertilizers, seaweed extracts are biodegradable, non-polluting, non-toxic and non-hazardous to humans and animals2.…”

mentioning

confidence: 99%

Effect of Kelp Waste Extracts on the Growth and Development of Pakchoi (Brassica chinensis L.)

Zheng

Jiang

et al. 2016

Sci Rep

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To explore the effects of kelp waste extracts (KWE) on the growth and development of Brassia chinensis L., germination and greenhouse experiments were carried out under different concentrations of KWE. The results showed that a higher germination percentage (95%), associated with high germination index (8.70), germination energy (71.67%) and seedling vigor index (734.67), was obtained under a lower KWE concentration (2%) compared with the control. The radicle length (4.97 cm), fresh weight (0.32 g/10 seedlings) and dry weight (0.015 g/10 seedlings) were significantly increased in the treatment of 2% KWE. KWE also could enhance the root growth, the maximum leaf length × width and the fresh weight of plants, the optimal value of which increased by 8.37 cm, 58.14 cm2 and 7.76 g under the treatment of 10% KWE compared with the control respectively. Meanwhile, the contents of vitamin C and soluble sugars in pakchoi leaf were improved by 19.6 mg/100 g and 1.44 mg/g compared with the control, and the nitrate content was decreased by 212.27 mg/kg. Briefly, KWE could markedly stimulate the pakchoi seeds germination at a lower concentration (2%) and enhance the plant growth and quality at a higher concentration (10%).

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“…However, the equal proportion of soil and vermiculite has been reported to work efficiently in plant initial establishment and growth (Mondal et al 2004). Earlier reports have revealed the significance of soil: vermiculite growth medium for successful seedling establishment in tomato (Khare et al 2010;Vinoth et al 2012;Sinha and Rajam 2013). Vinoth et al (2013) developed an efficient tissue culture independent Agrobacterium-mediated transformation system in tomato and argued that sterilized sand: soil: vermiculite (1:2:1) was proved to be the best growth medium for successful plantlet formation.…”

Section: Discussionmentioning

confidence: 99%

Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.)

Shah

Ali²,

Jan

et al. 2014

Plant Cell Tiss Organ Cult

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Cold is a major constraint for tomato growth and productivity; as it is a cold sensitive crop. DREB1A plays a key role in generating cold tolerance in tomato by regulating the response of multiple genes under chilling stress. In this study, cold tolerant gene (DREB1A) driven by Lip9 promoter, was transformed in three tomato genotypes through Agrobacterium tumefaciens, employing tissue culture independent transformation strategy. Overnight imbibed seeds of tomato were surface sterilized, 3-day old shoot apical meristem of the developing seedling were pierced and incubated for twenty minutes with A. tumefaciens strain EHA-105 having OD 600 nm of 1.0. The treated seeds were co-cultivated with Agrobacterium for 2 days. The regenerated shoots were subjected to 35 mg/l hygromycin as a selection for a period of 2-3 weeks. The presence of DREB1A and hpt genes in the hygromycin resistant (T 0 ) transgenic plants was evaluated by PCR analysis. The transgene activity was detected in T 1 plants by Reverse Transcriptase Polymerase Chain Reaction and Southern blotting that showed the stable integration of the transgene to the next generation. Physiological analysis of T 2 transgenic plants depicted that increased expression of DREB1A induced only during chilling stress. After various chilling stresses, stomatal conductance, transpiration rate and relative water contents of transgenic lines were significantly higher than those of NT plants. While leaf osmotic potential of transgenic plants was lower as compared to NT plants. The established procedure is novel and can produce stable transgenic tomato plants in efficient manner by saving potential resources in terms of cost and time.

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Effect of seaweed extracts and plant growth regulators on high-frequency in vitro mass propagation of Lycopersicon esculentum L (tomato) through double cotyledonary nodal explant

Cited by 48 publications

References 30 publications

The Use of Biostimulants for Enhancing Nutrient Uptake

The Use of Biostimulants for Enhancing Nutrient Uptake

Effect of Kelp Waste Extracts on the Growth and Development of Pakchoi (Brassica chinensis L.)

Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.)

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