Effect of Secretin on Mucinous Secretion by the Isolated Canine Stomach Perfused Extracorporeally

Abstract

Principal glycoproteins, uronic acid and amino acids were determined in canine gastric secretion, obtained from isolated canine stomachs perfused extracorporeally with homologous blood. Gastric secretion was collected during intraarterial infusion of pentagastrin alone for 3 h, and of secretin + pentagastrin for the next 3 h. Secretin produced a significant increase in the output of D-galactose, D-glucosamine, L-fucose, D-glucose, total sugars, total sulfate and uronic acid. Secretin had no significant effect on the output of most of amino acids of gastric secretion.

Composition of Gastric Mucinous Secretion from Heidenhain Pouches of Dogs Treated with Aspirin

“…It is interesting to note that the constant relation ships between galactose and glucosamine (close to 1/1) and between galactose and galactos amine (close to 2/1), found previously in human, procine and canine secretions (22), were also found in all experimental groups in the present study. This problem was discussed previously (20)(21)(22)(23)(24).…”

“…Production of mucin by the gastric mucosa has been extensively studied in clinical conditions and in experimental animals (1 6 -18). Various components of gastric mucinous secretion have been analyzed by a variety of methods under in vivo (1 6 ,1 9 -2 2 ) and ex vivo conditions (23,24).…”