Effect of seed sludge and operation conditions on performance and archaeal community structure of low-temperature anaerobic solvent-degrading bioreactors

Enright, Anne-Marie; McGrath, Vincent; Gill, Darryl; Collins, Gavin; O’Flaherty, Vincent

doi:10.1016/j.syapm.2008.10.003

Cited by 42 publications

(38 citation statements)

References 50 publications

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“…This result would indicate that the proteins present in our anaerobic sludge sample display a very limited solubility in the extraction buffer used in the French press protocol originally designed for activated sludge [4]. The flocculated activated sludge investigated by Wilmes and Bond [4] contained mainly bacteria (88.3%) while the granular anaerobic sludge used in the present study is typically dominated by archaeal species (75%; [10]). The sonication method initially tested in this study [6] was successfully applied on various anaerobic sludge samples.…”

Section: Many Years After Its Introduction By O'farrell In 1975mentioning

confidence: 82%

Optimisation of protein extraction and 2‐DE for metaproteomics of microbial communities from anaerobic wastewater treatment biofilms

2009

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The feasibility of metaproteomic analysis of the microbial communities underpinning anaerobic wastewater treatment relies on efficient protein extraction and separation techniques. The microorganisms involved in anaerobic digestion (AD) of wastewater typically aggregate to form tightly organised, spherical granules, from which protein extraction is challenging. Here, we compare two methods of protein extraction, one using a French press previously used successfully to analyse the proteome of an activated sludge [Wilmes, P., Bond, P. L., Environ. Microbiol. 2004, 6, 911-920.] and one using sonication developed in the context of pure culture [Abram, F., Wan-Lin, S., Wiedmann, M., Boor, K. J., Coote, P., Botting, C., Karatzas, K. A. G., O'Byrne, C. P., Appl. Environ. Microbiol. 2008, 74, 594-604.]. We used 2-DE to carry out this comparison. The protein extraction using the sonication method resulted in a significant increase in the number of protein spots and higher quality 2-D gels.

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Section: Many Years After Its Introduction By O'farrell In 1975mentioning

confidence: 82%

Optimisation of protein extraction and 2‐DE for metaproteomics of microbial communities from anaerobic wastewater treatment biofilms

2009

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“…All reactors produced a total of more than 2000 mL of biogas at 10th day 10. Seed sludge is known to impact the microbial community and the performance of the reactor on the start-up of anaerobic digestion [14]. Fig.…”

Section: Effect Of Seed Sludgementioning

confidence: 99%

“…After the 20-day digestion, the numbers of ARBs and total culturable bacteria in each digester were not significantly different among the samples of seed sludge, except for OTC-resistant bacteria (p <0.05). Enright et al [14] reported that several differences including microbial community were observed among the reactors seeded with different biomass sources. The result indicated that the reduction of the ARBs during batch anaerobic digestion of dairy manure was rarely influenced by the microbial community originated from seed sludge.…”

Section: Effect Of Seed Sludgementioning

confidence: 99%

Effect of Seed Sludge on Survival of Antibiotic-Resistant Bacteria and Fate of Bacteria Resistant to Different Classes of Antibiotics during Anaerobic Digestion of Dairy Manure

Takemura

Ihara

Kitazono

et al. 2016

J. of Wat. & Envir. Tech.

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Livestock manure is one of main pollution reservoirs of antibiotic-resistant bacteria (ARBs) due to excessive use of veterinary antibiotics. For preventing the environmental spread, an efficient process is required to reduce ARBs in livestock manure. In this work, we investigated the survival of ARB during mesophilic anaerobic digestion of dairy cattle manure. Effects of seed sludge and the antibiotic class were investigated for reduction of ARBs by batch digestion tests. The numbers of all ARBs were 6.34×10 4 to 2.08×10 6 before anaerobic digestion. The numbers of oxytetracycline, chlortetracycline, gentamicin and kanamycin -resistant bacteria decreased close to the detection limit after 20-day anaerobic digestion. However, reduction effects of cefazolin and streptomycin -resistant bacteria were small. The seed sludge had little influence on the survival of ARBs during the anaerobic digestion. The results showed that the ARB reduction effect was influenced by the types of antibiotics, and mostly not by seed sludge.

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“…Influences of various environmental parameters on community structure include the following: (1) higher influent SO4 −2 concentration leads to higher sulfate-reducing bacteria levels (Raskin et al, 1996 andPender et al, 2004), (2) higher digester acetate concentration leads to higher Methanosarcina and lower Methanosaeta abundance ( Griffin et al, 1997 andMcMahon et al, 2001), (3) NH3-N concentrations greater than approximately 3 g/L leads to lower Methanosarcina levels, higher Methanomicrobiales levels ( Angenent et al, 2002) and a shift from acetoclastic methanogenesis to syntrophic acetate oxidation with hydrogenotrophic methanogenesis (Fotidis et al, 2013), (4) lower temperature leads to higher diversity at 37 °C versus 55 °C (Karakashev et al, 2005) and sometimes leads to a shift from acetoclastic methanogenesis to hydrogenotrophic methanogenesis at psychrophilic temperatures ( Enright et al, 2009 andZhang et al, 2012), (5) different substrates lead to different community structures, including manure versus wastewater sludge (Karakashev et al, 2005) and glucose versus whey permeate and sewage sludge (Lee et al, 2009), and (6) trace nutrient deprivation causes a shift in community structure, with low cobalt or nickel concentrations causing decreased Methanosarcina abundance and decreased COD removal rate in methanol-fed bioreactors ( Fermoso et al, 2008a andFermoso et al, 2008b).…”

Section: Influence Of Environmental Parameters On Digester Microbial mentioning

confidence: 99%

Relating methanogen community structure and anaerobic digester function

Bocher¹,

Cherukuri

Maki

et al. 2015

Water Research

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Abstract:Much remains unknown about the relationships between microbial community structure and anaerobic digester function. However, knowledge of links between community structure and function, such as specific methanogenic activity (SMA) and COD removal rate, are valuable to improve anaerobic bioprocesses. In this work, quantitative structure-activity relationships (QSARs) were developed using multiple linear regression (MLR) to predict SMA using methanogen community structure descriptors for 49 cultures. Community descriptors were DGGE demeaned standardized band intensities for amplicons of a methanogen functional gene (mcrA). First, predictive accuracy of MLR QSARs was assessed using cross validation with training (n = 30) and test sets (n = 19) for glucose and propionate SMA data. MLR equations correlating band intensities and SMA demonstrated good predictability for glucose (q 2 = 0.54) and propionate (q 2 = 0.53). Subsequently, data from all 49 cultures were used to develop QSARs to predict SMA values. Higher intensities of two bands were correlated with higher SMA values; high abundance of methanogens associated with these two bands should be encouraged to attain high SMA values. QSARs are helpful tools to identify key microorganisms or to study and improve many bioprocesses. Development of new, more robust QSARs is encouraged for anaerobic digestion or other bioprocesses, including nitrification, nitritation, denitrification, anaerobic ammonium oxidation, and enhanced biological phosphorus removal. Graphical abstract

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Effect of seed sludge and operation conditions on performance and archaeal community structure of low-temperature anaerobic solvent-degrading bioreactors

Cited by 42 publications

References 50 publications

Optimisation of protein extraction and 2‐DE for metaproteomics of microbial communities from anaerobic wastewater treatment biofilms

Optimisation of protein extraction and 2‐DE for metaproteomics of microbial communities from anaerobic wastewater treatment biofilms

Effect of Seed Sludge on Survival of Antibiotic-Resistant Bacteria and Fate of Bacteria Resistant to Different Classes of Antibiotics during Anaerobic Digestion of Dairy Manure

Relating methanogen community structure and anaerobic digester function

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