Effect of selenium-rich Bacillus subtilis against mercury-induced intestinal damage repair and oxidative stress in common carp

Shang, Xinchi; Yu, Peng; Yin, Yuanyuan; Zhang, Yue; Lu, Yuting; Mao, Qiaohong; Li, Yuehong

doi:10.1016/j.cbpc.2020.108851

Cited by 23 publications

(20 citation statements)

References 45 publications

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“…Changes in the intestinal ora can lead to disorders of the body's normal physiological functions, leading to diseases (Nicholson et al, 2012). Through previous studies, we found that Hg signi cantly reduced the activity of enzymes such as CAT and GSH-PX and triggered in ammation (Shang et al, 2021). This experiment uses Illumina high-throughput sequencing technology to explain how the composition and diversity of carp intestinal microbial communities change under Hg exposure conditions, and provide a theoretical basis for sh intestinal health and normal human growth and development.…”

Section: Discussionmentioning

confidence: 90%

“…In addition, the up-regulation of IL-10 in the liver may represent an aspect of the homeostatic mechanism that controls the Hg-induced in ammatory response. Gao et al reported that the reduction of TGF-β will aggravate the in ammatory damage of liver tissue, but the lack of Se will inhibit the expression of TGF-β and promote the production of TNF-α, IL-1β and IL-6, which may cause carp liver tissue In ammation, but Se supplementation can prevent the decrease of TGF-β (Gao et al, 2019).The intake of Se-rich B. subtilis will not only increase the Se content in the body, but also B. subtilis will absorb Hg and alleviate the damage of the sh (Shang et al, 2021). In this study, there may be such a mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…Commercial feed as a basic diet. Se-rich B. subtilis is added to the basic feed For the detailed steps of preparing Se-enriched B. subtilis, please refer to Xinchi S et al (Shang et al, 2021). The mercury content in 20 ml water samples from different aquariums was collected.…”

Section: Preparing Se-rich B Subtilis and Preparing Dietmentioning

confidence: 99%

“…Table 1 displays the actual mercury concentration. The probiotics are diluted with sterile normal saline, fully homogenized and added to the basic feed according to the needs of the experiment ( nal dose of bacteria: 105 cfu/g feed; nal concentration of Se: 0.5 ppm) (Shang et al, 2021). The same volume of sterile saline was added to the basic diet to prepare a control.…”

Section: Preparing Se-rich B Subtilis and Preparing Dietmentioning

confidence: 99%

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Effects of Selenium-enriched Bacillus Subtilis on Growth Inflammatory and Intestinal Microbes of Common Carp Induced by Mercury

Shang¹,

Wang²,

Sun³

et al. 2021

Preprint

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Mercury (Hg) is a global pollutant that affects the health of humans and ecosystems. Selenium (Se) is an essential trace element for many organisms including humans. Bacillus subtilis is widely distributed in nature, is one of the main probiotics used in aquaculture, and has a certain adsorption effect on heavy metals. The interaction between Hg and Se was rigorously studied, especially due to the observation of the protective effect of Se on Hg toxicity. The common carp was exposed to Hg (0.03 mg/L), and 105 cfu/g Se-rich B. subtilis was added to the feed. After 30 days of feeding, samples were taken to evaluate the growth performance, serological response, inflammatory response, and intestinal microbial changes. In this study, when fish were exposed to Hg, the growth performance of the Se-rich B. subtilis plus 0.03 mg/L Hg fish group was lower than that of the control group and higher than 0.03 mg/L Hg; The levels of LZM and IgM decreased, but after supplementation with Se-rich B. subtilis, the levels of LZM and IgM increased; Hg treatment significantly up-regulated the mRNA expression of IL-1β, IL-8, TNF-α and NF-κB P65, but down-regulated the mRNA expression of IL-10, TGF-β and IkBα. However, compared with the Hg group, the Se-rich B. subtilis plus Hg group can significantly increase the mRNA expression levels of IL-1β, IL-8, TNF-α and NF-κB P65, but reduce the regulation of IL-10, TGF-β and IkBα expression. At the genus level, the abundance of Aeromonas in the intestines of common carp in the Hg treatment group increased, and Se-rich B. subtilis could reduce the abundance of Aeromonas (pathogenic bacteria). Through the analysis of the species, we found that the Hg group was mainly composed of Aeromonas sobria and Aeromonas hydrophila. However, in the Se-rich B. subtilis treatment group, we found that Aeromonas sobria was significantly less than the Hg group. Because Aeromonas (pathogenic bacteria) is harmful to the fish, it can induce inflammation in the fish and make the fish sick. Through microbiological analysis, it is found that Se-rich B. subtilis improves Hg-induced intestinal microbial changes, alleviates the abundance of Aeromonas, and alleviates the inflammation of the fish.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Preparing Se-rich B Subtilis and Preparing Dietmentioning

confidence: 99%

Section: Preparing Se-rich B Subtilis and Preparing Dietmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Selenium-enriched Bacillus Subtilis on Growth Inflammatory and Intestinal Microbes of Common Carp Induced by Mercury

Shang¹,

Wang²,

Sun³

et al. 2021

Preprint

Self Cite

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show abstract

“…SOD is a vital antioxidant enzyme and can eliminate excess free radicals, reduce and inhibit lipid peroxidation, and protect cells from oxidative damage [70,71]. GSH-Px is a vital peroxidase widely existing in the body, which can protect the structure and function of cell membranes from interference and damage of oxides because it can reduce the toxic peroxides to nontoxic hydroxyl compounds [72][73][74]. In the present study, the SOD and GSH-Px activities were significantly improved with the increase of MI supplementation regardless of dietary carbohydrate levels.…”

Section: Discussionmentioning

confidence: 99%

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Zhu

Pan

Wang

et al. 2020

Animals

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This study investigated the effect of dietary myo-inositol (MI) on alleviating the adverse effect of the high carbohydrate diet in Nile tilapia (Oreochromis niloticus). Six diets contained either low carbohydrate (LC 30%) or high carbohydrate (HC 45%) with three levels of MI supplementation (0, 400 and 1200 mg/kg diet) to each level of the carbohydrate diet. After an 8-week trial, the fish fed 400 mg/kg MI under HC levels had the highest weight gain and fatness, but the fish fed 1200 mg/kg MI had the lowest hepatosomatic index, visceral index and crude lipid in the HC group. The diet of 1200 mg/kg MI significantly decreased triglyceride content in the serum and liver compared with those fed the MI supplemented diets regardless of carbohydrate levels. Dietary MI decreased triglyceride accumulation in the liver irrespective of carbohydrate levels. The content of malondialdehyde decreased with increasing dietary MI at both carbohydrate levels. Fish fed 1200 mg/kg MI had the highest glutathione peroxidase, superoxide dismutase, aspartate aminotransferase and glutamic-pyruvic transaminase activities. The HC diet increased the mRNA expression of key genes involved in lipid synthesis (DGAT, SREBP, FAS) in the fish fed the diet without MI supplementation. Dietary MI significantly under expressed fatty acid synthetase in fish fed the HC diets. Moreover, the mRNA expression of genes related to lipid catabolism (CPT, ATGL, PPAR-α) was significantly up-regulated with the increase of dietary MI levels despite dietary carbohydrate levels. The gene expressions of gluconeogenesis, glycolysis and MI biosynthesis were significantly down-regulated, while the expression of the pentose phosphate pathway was up-regulated with the increase of MI levels. This study indicates that HC diets can interrupt normal lipid metabolism and tend to form a fatty liver in fish. Dietary MI supplement can alleviate lipid accumulation in the liver by diverging some glucose metabolism into the pentose phosphate pathway and enhance the antioxidant capacity in O. niloticus.

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Advances in the Study of Selenium‐Enriched Probiotics: From the Inorganic Se into Se Nanoparticles

Xiao,

Li,

Huang

et al. 2023

Molecular Nutrition Food Res

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Selenium (Se) is a momentous metallic element that plays an irreplaceable role in biochemical activities. Se deficiency remains a nutritional challenge across the world. Organic Se supplementation is the most effective treatment means for Se deficiency. Organic Se transformed from Se‐enriched probiotics show outstanding excellent properties in antibacteria, anti‐oxidation, anti‐inflammation, and immunoregulation. Studying the influencing factors for Se enrichment capacity and enrichment mechanisms of Se‐enriched probiotics is conducive to the exploit of more potent Se‐enriched probiotics. Se‐enriched probiotics transform inorganic Se into Se nanoparticles (SeNPs), which have been widely used in animal husbandry and biomedical field. In this paper, the novel development of Se‐enriched probiotics is reviewed, and the bioactivities of SeNPs are assessed, so as to display their potential application prospects. The excellent role of SeNPs in anti‐oxidation is summarized, and the mechanism by which SeNPs improve Se deficiency and boost animal health is explained.

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Effect of selenium-rich Bacillus subtilis against mercury-induced intestinal damage repair and oxidative stress in common carp

Cited by 23 publications

References 45 publications

Effects of Selenium-enriched Bacillus Subtilis on Growth Inflammatory and Intestinal Microbes of Common Carp Induced by Mercury

Effects of Selenium-enriched Bacillus Subtilis on Growth Inflammatory and Intestinal Microbes of Common Carp Induced by Mercury

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Advances in the Study of Selenium‐Enriched Probiotics: From the Inorganic Se into Se Nanoparticles

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