Effect of self‐assembling peptide P₁₁‐4 on enamel erosion: AFM and SEM studies

Abstract

The aim of the present in vitro study was to evaluate the protective effect of self-assembling peptide P11 -4 (Curodont™ Protect/Credentis) on enamel erosion produced by a soft-drink, by using Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). Thirty human incisors were equally and randomly assigned to 6 groups. Group 1: intact enamel, group 2: soft drink, group 3: Curodont(™) Protect applied, group 4: Curodont(™) Protect applied + soft drink, group 5: soft drink + Curodont(™) Protect applied, group 6: soft drink + Curodont(™) Protect applied + soft drink. Specimens were observed through atomic force microscopy (AFM). The root mean-square roughness (Rrms) was obtained from the AFM images and the differences in the averaged values among the groups were analyzed by Shapiro-Wilk test in order to assess the normality of the distribution. Parametric ANOVA and post hoc Tuckey test were performed to assess the differences between the different groups. After demineralization process, enamel demonstrated a high degree of surface porosity. This morphological aspect was reflected in the increase of Rrms values. No significant differences (p > 0.05) were reported between intact enamel and enamel only treated with P11 -4 and between eroded enamel and enamel treated with P11 -4 and then demineralized. However significant differences (p < 0.05) were recorded when comparing softened enamel with softened enamel further remineralized with biomimetic self-assembling peptides and enamel treated with the protective paste between two acid attacks. The use of P11 -4 remineralizing may offer a degree of protection from enamel erosion. SCANNING 38:344-351, 2016. © 2015 Wiley Periodicals, Inc.

Remineralization of Early Carious Lesions using Biomimetic Selfassembling Peptides Versus Fluoride agent. (In vitro and In vivo study)

“…Moreover, the results of the Ra of the present study came in accordance with a study that found significant reduction in enamel roughness values on comparing eroded enamel and eroded enamel treated with Curodont (25) .…”

“…Curodont repair is a product that incorporates the P11-4-based technology, together with fluoride and calcium phosphate (25) . Therefore, Curodont produced constant and stable remineralization of all demineralized areas, allowing continuous minerals supply for the tooth, which filled deeply in the porosities formed after demineralization.…”

Self‐assembling peptide matrix for treatment of dentin hypersensitivity: A randomized controlled clinical trial

“…In the present study, both the test product, containing SAPM, and the control product, containing ACC, provided significant relief of DHS, as expected from the literature investigating the products and the observed tubule occlusion in the SEM experiments presented as part of this study . Differences between the groups were mostly not statistically significant; as the study was designed as a pilot study, no power analysis could be performed due to the novelty of the SAPM product.…”

“…1). Because the SAPM has Ca 2+ binding sites, the layer on the dentin surface is expected to attract calcium ions from saliva‐forming hydroxyapatite crystals, resulting in a stronger, more stable, layer that is less susceptible to abrasion …”

“…19 Recently, a new approach for occluding dentinal tubules has been reported 20 : self-assembling-peptide matrix (i.e., hydrogel) applied to the exposed root surface does not block the dentinal tubules from within, but through a protective layer on the exposed dentin. 20,21 As the occlusion does not depend on an additional chemical reaction, tubule occlusion can occur immediately. However, the drawback may be that because the hydrogel does not penetrate significantly into the tubule, the protective layer might be susceptible to abrasion.…”