Effect of Semen Collection in Extender Solution on the Characteristics of Goat Spermatozoa

Yamashiro, Hideaki; Kumamoto, Kanako; Wang, Hai Feng; Yamashita, Yugo; Takato, Tsuyoshi

doi:10.1262/jrd.17104

Cited by 20 publications

(19 citation statements)

References 33 publications

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“…The sperm cell motility of 70% and above obtained in this study was acceptable as it was within the range considered of high quality (Yamashiro et al, 2006) compared to the sperm cell motility of 62.5% as earlier reported using Saanen goats (Nur et al, 2005). Also, the sperm cell concentration of 104.4×10 7 /ml observed in Anglo-Nubian goat used was higher than the sperm cell concentrations (116.7×10 6 -663.6×10 6 /ml) reported in South African indigenous goat and Gorno Altai (Dombo, 2002;Ramukhithi et al, 2011) but lower compared to sperm concentration of 3×10 9 /ml (Tuli and Holtz, 1995;Sundararaman et al, 2007) to 4.7×10 9 /ml (Hidalgo et al, 2006) in Florida goats.…”

Section: Resultssupporting

confidence: 85%

Sperm concentration influences the post-thaw livability of frozen goat semen

2016

Res. Opin. Anim. Vet. Sci.

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In this study, goat semen was cryopreserved in a reduced concentration of 75×10 6 , 50×10 6 and 25×10 6 sperm/ml and evaluated based on post-thaw motility and livability. The ejaculated semen samples were collected using an artificial vagina, extended with an egg yolk Tris extender, cryopreserved in straws using glycerol (7%) and egg yolk (5%) as permeating and non-permeating cryoprotectants in LN2. Post-thaw semen evaluation showed motility scores of 36.6±2.13%, 35.4±2.04%, 27.0±3.0% and 19.2±2.78% for the control, T-A (75×10 6 ), T-B (50×106) and T-C ( 25×10 6 ), respectively. The mean post-thaw percentage livability obtained in the control (60.6±4.39) was higher than T-A (42.86±1.83), T-B (39.36±2.86) and T-C (19.2±5.27). The percentage abnormal sperm in all treatment groups were less than 10%, indicating that the method used for freezing of goat semen in reduced concentration could be useful in artificial insemination and in vitro fertilization studies. The reduced sperm motility and livability after thawing merits further studies on the factors influencing the survivability of sperm during cryopreservation in creating a standardized and better freezing protocol for goat semen.

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Section: Resultssupporting

confidence: 85%

Sperm concentration influences the post-thaw livability of frozen goat semen

2016

Res. Opin. Anim. Vet. Sci.

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“…At ejaculation, ruminant seminal plasma contains factors that positively or negatively influence the fertilizing capacity of the spermatozoa (Yamashiro et al, 2006). An example of this negative interaction was observed in goat semen dilution, when the cryosurvival in extenders contained egg yolk (Roy, 1957) or milk (Nunes, 1982).…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of goat cauda epididymal sperm, cooled in different extenders at 4ºC

et al. 2013

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SUMMARYCollection of spermatozoa obtained from the epididymis cauda (CES) is a viable option to preserve of genetic material from threatened species and for use in assisted reproduction. The aim of the present study was to assess the in vitro effect of four different extenders on spermatozoa from goat epididymis cauda, cooled at 4 ºC. Epididymal sperm were recovered from the cauda by flushing six pairs of epididymis. The samples of each pair were mixed, subdivided into four aliquots and diluted in coconut water-egg yolk (CW-EY), physiologic solution with 0.5 % glucose and egg yolk (PSG-EY), UHT skim milk (SM), and UHT skim milk with egg yolk (SM-EY). The samples were cooled at 4 ºC for 2, 12, 24 and 48 hours. After storage, in each period, the semen was evaluated by the heat resistance test for sperm motility, vigor and total morphological alterations. The motility degradation rate was calculated at the end of each time period. Sperm viability decreased with time in the four extenders. Epididymal sperm diluted in CW-EY showed higher values for vigor and motility, although these parameters did not differ from the sperm kept in the PSG-EY up to 48 hours at 4 °C, except for spermatic vigor. However, the PSG-EY and SM extenders caused greater morphologic damage to epididymal sperm after 12 and 24 hours, respectively. Extenders based on skim milk provided less spermatic cell stability during 48 hours. In conclusion, CW-EY extender was the most efficient extender to maintain CES viability at 4 ºC. RESUMOA colheita de espermatozóides da cauda do epidídimo (CES) é uma opção viável de preservação de material genético de espécies ameaçadas de extinção, bem como para uso em reprodução assistida. Este trabalho teve como objetivo verificar a eficiência de quatro diferentes diluidores sobre os espermatozóides da cauda do epidídimo de caprinos, resfriado a 4 ºC. Os espermatozoides epididimários foram recuperados da cauda de seis pares de epidídimo. As amostras de cada par foram misturadas e subdivididas em quatro alíquotas que foram diluídas na água de cocogema (CW-EY), solução fisiológica glicosada 0,5 %-gema (PSG), leite desnatado UHT (ME) e leite desnatado UHT-gema (ME-EY). As amostras foram resfriadas a 4 °C por 2, 12, 24 e 48 horas. Após o armazenamento, em cada período, o CES foi avaliado através do teste de termoresistência quanto à motilidade, vigor espermático e alterações morfológicas total. A taxa de degradação da motilidade foi calculada no final de cada período. A viabilidade espermática diminuiu com o tempo de refrigeração nos quatro diluidores. Os CES diluídos na CW-EY apresentaram maiores valores para vigor e motilidade, embora estes parâmetros não deferissem daqueles mantidos no PSG por até 48 horas, exceto para vigor espermático. Entretanto, os diluidores PSG e ME ocasionaram maiores danos morfológicos aos CES a partir de 12 e 48 horas, respectivamente. Os diluidores ME e ME-EY proporcionaram à célula espermática menor estabilidade durante 48 h de conservação.Archivos de zootecnia vol. 62, núm. 239, p. 430. ...

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“…In vitro functional characteristics of goat sperm are abruptly modified by flash contact with accessory gland fluid at ejaculation [30] . Prostasomes are membranous vesicles secreted by the prostate gland that are surrounded by a bilaminar or multilaminar lipoprotein and contain large amounts of cholesterol, sphingomyelin, calcium and different enzymes [3] .…”

Section: Discussionmentioning

confidence: 99%

“…The proportion of SYBR-14 and PIstained sperm in each sample was then counted in more than 200 sperm and the result was expressed as the percentage of viability. The acrosome status of fresh and frozen-thawed sperm was assessed by staining with FITC-PNA (Sigma) using a modification of the method described by [30] . The percentage of intact acrosome was calculated after collection and freeze-thaw of 200 sperm that were randomly selected on each slide.…”

Section: Materials and Mathodsmentioning

confidence: 99%

Influence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog Sperm

Atsushi¹

2009

American J. of Animal and Veterinary Sciences

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Problem statement: Two experiments were conducted to determine the effect of the prostatic fluid from the first and third fractions of the ejaculates on the freezability of poodle dog sperm. Approach: The ejaculates from 2 trained poodle dogs were collected. Effect of prostatic fluid from the first and third fractions of ejaculates on cryosurvival of dog sperm was examined. SDS-PAGE analyses were performed to verify whether there were any modifications in the protein patterns of the first and third fractions. Results: When second fractions of Dog A sperm were combined with the prostatic fluid from the first and third fractions of the ejaculates, sperm motility index was decreased compared to second fractions were collected alone. The post-thaw motility of Dog A sperm was significantly reduced when prostatic fluid from the first and third fractions of the ejaculates was added. The motility of frozen-thawed Dog B sperm did not appear to be altered when it was diluted with either of the prostatic fluids. The major bands present in both prostatic fluids corresponded to proteins with molecular masses of 30 and 75 kDa. The amount of proteins in the prostatic fluid from the third fraction was twice-three times as much protein present compared to the first fraction. Conclusion: Collection of the sperm-rich fraction along with either the prostatic fluid from the 1st and 3rd fractions of the ejaculates during sperm processing has varying detrimental effects on the motility characteristics of frozen-thawed sperm.

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Effect of Semen Collection in Extender Solution on the Characteristics of Goat Spermatozoa

Cited by 20 publications

References 33 publications

Sperm concentration influences the post-thaw livability of frozen goat semen

Sperm concentration influences the post-thaw livability of frozen goat semen

In vitro evaluation of goat cauda epididymal sperm, cooled in different extenders at 4ºC

Influence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog Sperm

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