Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile Saudi males

Fatima, Sabiha; Alwaznah, Raheek; Aljuraiban, Ghadeer S.; Wasi, Samina; Abudawood, Manal; Abulmeaty, Mahmoud M. A.; Berika, Mohamed; Aljaser, Feda

doi:10.15537/smj.2020.3.24975

Cited by 12 publications

(7 citation statements)

References 36 publications

(44 reference statements)

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“…A significant negative correlation was observed between sperm parameters and 4‐HNE. The results agree with the findings of Fatima et al (2020 ) where a strong negative correlation of sperm motility and morphology with 4‐HNE was observed.…”

Section: Discussionsupporting

confidence: 93%

Male infertility: Role of vitamin D and oxidative stress markers

et al. 2021

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Spermatozoa are vulnerable to oxidative stress because of their inherent reduced antioxidant defence and DNA repair mechanisms. Polyunsaturated fatty acids in sperm plasma membrane break down to cytotoxic lipid aldehyde, 4‐Hydroxynonenal, whereas 3‐Nitrotyrosine is generated by peroxynitrite induced tyrosine nitration. Both oxidative stress markers contribute to altered sperm function and infertility. Vitamin D, a membrane antioxidant, has a potential scavenger capacity. We compared oxidative stress markers and vitamin D in male subjects with normal and altered sperm parameters and explored association of these markers: 4‐Hydroxynonenal and 3‐Nitrotyrosine with Vitamin D. Higher 4‐Hydroxynonenal levels in altered sperm parameter group and a negative correlation with sperm count, motility and morphology (p < 0.001) was observed. Vitamin D serum concentration in altered sperm parameters was less (p = 0.016) showing a significant positive correlation with sperm count and morphology. 4‐Hydroxynonenal was significantly higher in altered sperm parameters showing negative correlation with vitamin D. Highest serum concentrations of 4‐Hydroxynonenal were observed in vitamin D‐deficient subjects. Significantly higher concentration of 4‐Hydroxynonenal was estimated in altered sperm parameters of vitamin D sufficient group (p < 0.001). This suggests 4‐Hydroxynonenal as an oxidative stress marker leading to altered sperm function and infertility with some association with vitamin D; needs to be explored.

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Section: Discussionsupporting

confidence: 93%

Male infertility: Role of vitamin D and oxidative stress markers

et al. 2021

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“…The production of 4HNE causes mitochondrial injury, and eventually results in DNA damage and apoptosis. The detection methods of 4HNE include flow cytometry, ELISA kit, mass spectrometry, and proteomic approach [ 132 , 133 , 134 , 135 ]. Since sperm mitochondria is an important source of ROS and the organelle that is susceptible to it, measurement of mitochondrial ROS production and mitochondrial damage is widely used for sperm quality evaluation during liquid storage [ 85 , 136 ].…”

Section: Effects Of Extended Non-freezing Storage Of Human Spermatozo...mentioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

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Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role in reproductive biology and medicine. However, it is well known that human spermatozoa left in vitro deteriorate over time irreversibly as the consequence of various stresses such as the change of osmolarity, energy deficiency, and oxidative damage, leading to substantial limitations including the need for semen examinations, fertility preservation, and assisted reproductive technology. These problems may be addressed with the aid of non-freezing storage techniques. The main and most effective preservation strategies are the partial or total replacement of seminal plasma with culture medium, named as extenders, and temperature-induced metabolic restriction. Semen extenders consist of buffers, osmolytes, and antioxidants, etc. to protect spermatozoa against the above-mentioned adverse factors. Extended preservation of human spermatozoa in vitro has a negative effect on sperm parameters, whereas its effect on ART outcomes remains inconsistent. The storage duration, temperature, and pre-treatment of semen should be determined according to the aims of preservation. Advanced techniques such as nanotechnology and omics have been introduced and show great potential in the lifespan extension of human sperm. It is certain that more patients will benefit from it in the near future. This review provided an overview of the current knowledge and prospects of prolonged non-freezing storage of human sperm in vitro.

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“…This can be explained by the increased number of apoptotic sperm cells due to oxidative stress in oligozoospermic men and to high oxidation–reduction potential in the seminal plasma [ 66 ]. Oxidative damage to DNA, key metabolic proteins, and plasmatic membrane are known triggers of apoptosis [ 67 , 68 ] that is upregulated in oligozoospermic men. This could make one assume that oligozoospermic men are the ones with more oxidative stress in the semen.…”

Section: Seminal Plasma Antioxidant Capabilities and Relation To Male Fertility Outcomesmentioning

confidence: 99%

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

et al. 2021

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Nowadays, infertility is classified as a disease of the reproductive system. Although it does not compromise the life of the individual, it can have detrimental effects on the physiological and psychological health of the couple. Male fertility evaluation is mainly focused on the analysis of sperm parameters. However, the ejaculated fluid is also composed of seminal plasma, and the study of this fluid can provide crucial information to help in the assessment of male fertility status. Total antioxidant capacity of the seminal plasma has been positively correlated with the fertility of men. Moreover, evidence highlights to a similar importance as that of female reproductive tract fluid antioxidant capabilities and female fertility. Herein, we describe the functions of seminal plasma and female reproductive tract fluids, as well as their main antioxidant components and their relationships with fertility outcomes. Additionally, this review contains the most up to date information regarding the mechanisms of the interaction between the male and the female reproductive fluids and the importance of proper antioxidant capacity for fertilization.

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Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile Saudi males

Cited by 12 publications

References 36 publications

Male infertility: Role of vitamin D and oxidative stress markers

Male infertility: Role of vitamin D and oxidative stress markers

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

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