Effect of serine-type protease of Candida spp. isolated from linear gingival erythema of HIV-positive children: critical factors in the colonization

Portela, Maristela Barbosa; Souza, Ivete Pomarico Ribeiro de; Abreu, Celina Monteiro; Bertolini, Martinna; Holandino, Carla; Alviano, Celuta Sales; Santos, André Luis Souza dos; Soares, R. Félix

doi:10.1111/j.1600-0714.2010.00906.x

Cited by 27 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…yeast carbon base -YCB) supplemented with protein (e.g., BSA or hemoglobin) as a unique source of nitrogenous compounds produce a large amount of Saps, particularly Sap2. On the other hand, C. albicans cells grown in nitrogen-unlimited media (e.g., brain heart infusion -BHI) robustly diminished the expression of SAP genes [73,[182][183][184][185]. These results pointed out to the metabolism versatility of this fungus and these properties can be used to test the effect of PIs on the ability of yeasts to degrade proteins in order to acquire amino acids for its nutrition, development and growth.…”

Section: Anti-proliferative Effects Of Hiv Pismentioning

confidence: 83%

Aspartic Protease Inhibitors as Potential Anti-Candida albicans Drugs: Impacts on Fungal Biology, Virulence and Pathogenesis

Braga-Silva

Santos

2011

CMC

View full text Add to dashboard Cite

Mycoses are still one of the most problematic illnesses worldwide, especially affecting immunocompromised individuals. The development of novel antifungal drugs is becoming more demanding every day, since existing drugs either have too many side effects or they tend to lose effectiveness due to the resistant fungal strains. In this scenario, Candida albicans is still the main fungal pathogen isolated in hospitals. Pathogenicity results essentially from modifications of the host defense mechanisms that secondarily initiate transformations in the fungal behavior. The pathogenesis of C. albicans is multifactorial and different virulence attributes are important during the various stages of infection. Some virulence factors, like the secreted aspartic proteases (Saps), play a role in several infection stages and the inhibition of one of the many stages may contribute to the containment of the pathogen and thus should help in the treatment of disease. Therefore, Saps are potential targets for the development of novel anti-C. albicans drugs. Herein, we review the beneficial properties of pepstatin A and aspartic-type protease inhibitors used in the anti-human immunodeficiency virus chemotherapy on C. albicans, with particular emphasis in the effects on Sap activity, proliferation, morphogenesis (yeasts into mycelia transformation), ultrastructural architecture, adhesion to mammalian cells and abiotic materials, modulation of unrelated virulence factors (e.g., surface glycoconjugates, lipases and sterol), experimental candidiasis infection as well as synergistic properties when conjugated with classical antifungals. Collectively, these positive findings have stimulated the search for novel natural and/or synthetic pharmacological compounds with anti-aspartic protease properties against the human opportunistic fungus C. albicans.

show abstract

Section: Anti-proliferative Effects Of Hiv Pismentioning

confidence: 83%

Aspartic Protease Inhibitors as Potential Anti-Candida albicans Drugs: Impacts on Fungal Biology, Virulence and Pathogenesis

Braga-Silva

Santos

2011

CMC

View full text Add to dashboard Cite

show abstract

“…cells with the serine protease inhibitor PMSF diminished the Candida spp. interaction with epithelial cells [21]. …”

Section: Discussionmentioning

confidence: 99%

A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins

et al. 2010

View full text Add to dashboard Cite

BackgroundParacoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi.ResultsA cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2.ConclusionsA secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.

show abstract

“…The observed molecular weight from the gel separations of the protease is 25 kDa, whereas the predicted size of Cwp1.2p is 20.7 kDa.The signal sequence weight (amino acids [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] has to be subtracted from the predicted value and the obvious extra weight of mannans/ oligosaccharides attached to the protein has to be added to the weight of this predicted protein. This explains the difference between the observed and predicted molecular weights.…”

Section: Discussionmentioning

confidence: 99%

“…glabrata is phylogenetically closer related to Saccharomyces cerevisiae (S. cerevisiae) than to Candida albicans (C. albicans) [6] and has a unique set of virulence mechanisms [7], such as phenotypic switching [8], and pseudohyphal formation [9], no secreted proteases but phospholipase activity [10], and adhesins [11]. The role of Candida proteases in virulence is still unclear, but there is evidence that strain-dependent variations exist among yeasts [12,13]. The function of one of the GPI-linked aspartyl proteases, CgYps1, has been recently shown to participate in the regulation of pH homeostasis in C. glabrata [14].…”

Section: Introductionmentioning

confidence: 99%

A novel Candida glabrata cell wall associated serine protease

Pärnänen

Meurman

Nikula-Ijäs

2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Effect of serine-type protease of Candida spp. isolated from linear gingival erythema of HIV-positive children: critical factors in the colonization

Abstract: Collectively, our results show that Candida spp. isolated from LGE lesions produced and secreted serine proteases and these enzymes may be involved in the initial colonization events.

Cited by 27 publications

References 45 publications

Aspartic Protease Inhibitors as Potential Anti-Candida albicans Drugs: Impacts on Fungal Biology, Virulence and Pathogenesis

Aspartic Protease Inhibitors as Potential Anti-Candida albicans Drugs: Impacts on Fungal Biology, Virulence and Pathogenesis

A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins

A novel Candida glabrata cell wall associated serine protease

Contact Info

Product

Resources

About