Effect of Sex and Assay Method on Serum Concentrations of Growth Hormone in Patients with Acromegaly and in Healthy Controls

Abstract

/L), however, the difference was ϳ2-fold. Overall, the Orion RIA method also showed a good correlation (r ‫؍‬ 0.951-0.959) with the other methods, but it did not

Quantification of growth hormone in serum by isotope dilution mass spectrometry

“…Though sufficient for GH quantification in materials presently used in quality control schemes and also in clinical samples seen during stimulation tests, improvement of the LOQ by about one order of magnitude would be required to extend the applicability to measure samples in patients during glucose suppression tests, where GH concentrations less than 1 µg/L are expected [32]. In terms of signal recovery, gaining a factor of 4-8 might be expected through complete elimination of ion suppression effects by further improvement of the clean-up procedure.…”

Gender differences in serum GH and IGF-I levels and the GH response to dynamic tests in patients with acromegaly

“…Whether the GH response to a glucose load is dependent on gender has been investigated [18,19,20,21]. Serum GH concentrations are lower in male than in female both at baseline and at the post glucose GH nadir in patients with acromegaly as well as in normal subjects [19,20,21].…”

“…Serum GH concentrations are lower in male than in female both at baseline and at the post glucose GH nadir in patients with acromegaly as well as in normal subjects [19,20,21]. Consequently, there are no gender differences in the percentage of GH suppression after a glucose load either in normal subjects or in those with acromegaly and a residual tumor after transsphenoidal surgery [19,22].…”

Growth hormone-binding protein is directly and IGFBP-3 is inversely associated with risk of female breast cancer

“…A recent study (16) of premenopausal women failed to find an association between breast cancer and GH but did not examine GHBP. GH itself is a difficult marker to study because of its pulsatile secretion and circadian variability and heterogeneity of available assays, which can increase the complexity of GH quantification especially at the low range of GH concentrations (41)(42)(43)(44)(45)(46). Thus, a single serum GH determination is not adequate for assessing GH levels in epidemiology studies.…”