Effect of shear stress on the formation of bacterial biofilm in a microfluidic channel

Park, Aeri; Jeong, Heon‐Ho; Lee, Jintae; Kim, Keun Pil; Lee, Chang‐Soo

doi:10.1007/s13206-011-5307-9

Cited by 90 publications

(69 citation statements)

References 22 publications

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“…A positive aspect is that the biofilm formation or cell attachment is strongly dependent on the shear stresses. As mentioned in previous studies as well (Park et al, 2011) at higher local velocities, biofilm formation rate decreases drastically. This is a favorable aspect in terms of conformance control and undesired plugging around the wellbore.…”

Section: Wettability Changesupporting

confidence: 81%

An Integrated German MEOR Project, Update: Risk Management and Huff'n Puff Design

Alkan¹,

Klueglein

Mahler

et al. 2016

SPE Improved Oil Recovery Conference

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This paper provides an update on a microbial enhanced oil recovery (MEOR) project conducted by Wintershall and BASF. Overall nutrient development and planning of a single well field trial (huff'n'puff, HnP) including risk management are described. A nutrient solution is tailored to stimulate growth and metabolite production of a reservoir community of various indigenous microbial species in a Wintershall operated oil field with challenging reservoir characteristics, including high salinity (160,000 ppm). Up-scaled imbibition experiments performed with sandstone cores using MEOR-oil systems are compared with injection brine-oil systems and assessed for the implications on incremental oil. The results of sandpack and coreflood experiments performed with optimized nutrient solutions are discussed regarding incremental oil recovery and responsible EOR mechanisms. A MEOR modelling concept developed using STARS/CMG is used to estimate additional oil production under various feeding strategies after the calibration of the EOR mechanisms assigned.As the laboratory and numerical works have indicated the feasibility of the MEOR field application, emphasis has been put on risk issues ranked in the register of the project. The key risk is potential souring of the reservoir due to the activation of the sulphate reducing bacteria (SRB) growing on the metabolites generated by the MEOR target community. Conventional mitigation measures have been tested in short and long-term experiments. An innovative solution had been developed to assure H 2 S free application without any consequences to the reservoir and to the MEOR application.A single well pilot application is planned in a pre-selected well of the Wintershall field studied with two main objectives: (1) proof of the concept of risk mitigation and (2) stimulation of growth and metabolite production. Identification of operational issues as well as data gathering to improve the forecasting methods towards full-field predictions are secondary objectives. A monitoring plan has been initiated to establish a baseline in terms of microbiological and petro-dynamic parameters. Temperature and volumetric distributions have been predicted based on the results of an injectivity test performed in the well. The data is used to design the HnP operation and the surface setup for the injection rate of 100 m 3 /day nutrient solution under well-defined conditions.

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Section: Wettability Changesupporting

confidence: 81%

An Integrated German MEOR Project, Update: Risk Management and Huff'n Puff Design

Alkan¹,

Klueglein

Mahler

et al. 2016

SPE Improved Oil Recovery Conference

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“…Both Δ cypS and Δ lfiA Δ lfiB mutants had a zigzagged phenotype and were unable to grow in liquid culture. The zigzagged mutants provide more accessible surface for the acting mechanical forces in liquid 63 , including fluid shear stress 64 , ultimately resulting in forces that cannot be endured by the abnormal mutant trichomes. Notably, while the selective advantage of cell shape is considered to be mostly a manifestation of biotic and abiotic selective factors in the cell environment 65 , the selective advantage of multicellular shapes is likely related to the efficiency of intercellular communication and transport 63 .…”

Section: Discussionmentioning

confidence: 99%

Two novel heteropolymer-forming proteins maintain multicellular shape of the cyanobacteriumAnabaenasp. PCC 7120

Springstein

Nürnberg²,

Kieninger

et al. 2019

Preprint

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23The determinants of bacterial cell shape are extensively studied in unicellular forms. 24Nonetheless, the mechanisms that shape bacterial multicellular forms remain understudied. 25Here we study coiled-coil rich proteins (CCRPs) in the multicellular cyanobacterium Anabaena 26 sp. PCC 7120 (hereafter Anabaena). Our results reveal two CCPRs, termed LfiA and LfiB (for 27 linear filament), which assemble into a heteropolymer that traverses the longitudinal cell axis. 28Two additional CCRPs, CypS (for cyanobacterial polar scaffold) and CeaR (for cyanobacterial 29 elongasome activity regulator), form a polar proteinaceous scaffold and regulate MreB activity, 30 respectively. Deletion mutants of these CCRPs are characterized by impaired filament shape 31 and decreased viability. Our results indicate that the four CCRPs form a proteinaceous network 32 that stabilizes the Anabaena multicellular filament. We propose that this cytoskeletal network 33 is essential for the manifestation of the linear filament phenotype in Anabaena. 34 (a multi-enzyme complex), is a regulator of longitudinal PG biogenesis, and thus it plays a 49 crucial role in the adaption to different environments and prokaryotic multicellularity. 50The key hallmarks of permanent bacterial multicellularity are morphological 51 differentiation and a well-defined and reproducible shape, termed patterned multicellularity 3 . 52Unlike biofilms, patterned multicellular structures are the result of either coordinated swarming 53 or developmental aggregation behavior as in myxobacteria 9 . Additional factors include cell 54 division, proliferation and cell differentiation as in sporulating actinomycetes 10 and 55 cyanobacterial filaments 11,12 . In myxobacteria as well as in actinomycetes, it has been shown 56 that patterned multicellular traits are dependent on the coordinated function of different coiled-57 coil-rich proteins (CCRPs). Reminiscent of eukaryotic intermediate filaments (IFs) 13,14 , many 58 bacterial CCRPs were shown to perform analogous cytoskeletal functions through their ability 59 to self-assemble into distinct filaments in vitro and in vivo [15][16][17][18][19] . Unlike FtsZ or MreB 20,21 , 60 bacterial IF-like CCRPs do not require any additional co-factors for polymerization in vitro 22 . 61For example, in Myxococcus xanthus, the coordinated swarming and aggregation into fruiting 62 bodies is mediated by its gliding motility 23 , which strictly depends on the filament-forming 63 CCRP AglZ 24 . AglZ is organized in a large multi-protein complex that governs gliding motility 64 in synergy with MreB, which still retained its PG synthesis function but was also co-opted for 65 gliding motility in M. xanthus [25][26][27][28] . Actinobacteria, such as Streptomyces species, grow by 66 building new cell wall (i.e. PG) only at the cell poles, independent of MreB 29,30 , which is 67 strikingly different from how most other bacteria grow 31 . This characteristic polar growth mode 68 is organized by a cytoskeletal network of at least three CCRPs -DivIVA...

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“…Lee et al [83] determined the spatial alterations under shear stress inflicted in a PDMS device exhibited by biofilms. During the dispersion stages or biofilm development in mature biofilms shear stress impacts negatively; although, throughout the initial moreover adhesion periods, shear stress facilitates biofilm development by proposing more nutrients as well as a chance for dispersion [84]. Lack of stress facilitates the growth of a bacterial cell; biofilms development gradually correlates to the growth of cells under stressful situations.…”

Section: Microfluidic Study Of Microbial Biofilmmentioning

confidence: 99%

Advancements and Potential Applications of Microfluidic Approaches—A Review

Ahmed¹,

Akram²,

Bule

et al. 2018

Chemosensors

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A micro-level technique so-called “microfluidic technology or simply microfluidic” has gained a special place as a powerful tool in bioengineering and biomedical engineering research due to its core advantages in modern science and engineering. Microfluidic technology has played a substantial role in numerous applications with special reference to bioscience, biomedical and biotechnological research. It has facilitated noteworthy development in various sectors of bio-research and upsurges the efficacy of research at the molecular level, in recent years. Microfluidic technology can manipulate sample volumes with precise control outside cellular microenvironment, at micro-level. Thus, enable the reduction of discrepancies between in vivo and in vitro environments and reduce the overall reaction time and cost. In this review, we discuss various integrations of microfluidic technologies into biotechnology and its paradigmatic significance in bio-research, supporting mechanical and chemical in vitro cellular microenvironment. Furthermore, specific innovations related to the application of microfluidics to advance microbial life, solitary and co-cultures along with a multiple-type cell culturing, cellular communications, cellular interactions, and population dynamics are also discussed.

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Effect of shear stress on the formation of bacterial biofilm in a microfluidic channel

Cited by 90 publications

References 22 publications

An Integrated German MEOR Project, Update: Risk Management and Huff'n Puff Design

An Integrated German MEOR Project, Update: Risk Management and Huff'n Puff Design

Two novel heteropolymer-forming proteins maintain multicellular shape of the cyanobacteriumAnabaenasp. PCC 7120

Advancements and Potential Applications of Microfluidic Approaches—A Review

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