Effect of short-term application of nitrogen on the accumulation of β-subunit of β-conglycinin in nitrogen-starved soybean (<i>Glycine max</i>L.) developing seeds

Ohtake, Norikuni; Kawachi, Tahei; Okuyama, Ikuko; Fujikake, Hiroyuki; Sueyoshi, Kuni; Ohyama, Takuji

doi:10.1080/00380768.2002.10409168

Cited by 9 publications

(2 citation statements)

References 34 publications

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“…The b-subunit of conglycinin exhibits plasticity in response to available sulfur (Holowach et al, 1986;Hirai et al, 1995;Tabe et al, 2002;Hagan et al, 2003). There is also feedback regulation of protein filling in the seed sink in response to nitrogen availability (Biermann et al, 1998;Ohtake et al, 2002). Another source of regulation is the overexpression of seed-specific amino acid permeases that increase the nutrient flux into the seed, resulting in an increase in seed sink protein content (Rolletschek et al, 2005).…”

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Silencing of Soybean Seed Storage Proteins Results in a Rebalanced Protein Composition Preserving Seed Protein Content without Major Collateral Changes in the Metabolome and Transcriptome

et al. 2011

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The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP2) seeds are overtly identical to the wild type, maturing to similar size and weight, and in developmental ontogeny. The SP2 seeds rebalance the proteome, maintaining wild-type levels of protein and storage triglycerides. The SP2 soybeans were evaluated with systems biology techniques of proteomics, metabolomics, and transcriptomics using both microarray and next-generation sequencing transcript sequencing (RNA-Seq). Proteomic analysis shows that rebalancing of protein content largely results from the selective increase in the accumulation of only a few proteins. The rebalancing of protein composition occurs with small alterations to the seed's transcriptome and metabolome. The selectivity of the rebalancing was further tested by introgressing into the SP2 line a green fluorescent protein (GFP) glycinin allele mimic and quantifying the resulting accumulation of GFP. The GFP accumulation was similar to the parental GFPexpressing line, showing that the GFP glycinin gene mimic does not participate in proteome rebalancing. The results show that soybeans make large adjustments to the proteome during seed filling and compensate for the shortage of major proteins with the increased selective accumulation of other proteins that maintains a normal protein content.

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Silencing of Soybean Seed Storage Proteins Results in a Rebalanced Protein Composition Preserving Seed Protein Content without Major Collateral Changes in the Metabolome and Transcriptome

et al. 2011

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“…The reduction in globulins might happen as a response to changes in the levels or flux of free amino acids in the transgenic lines. Transcript levels of the β‐subunit of β‐conglycinin have been shown to be regulated by exogenous glutamine in developing seeds grown in vitro (Ohtake et al , Hernández‐Sebastià et al ). Ohtake et al (), using treatments with the amides glutamine or asparagine in combination with inhibitors of aminotransferases or glutamate synthase, concluded that the transcript of the β‐subunit is highly sensitive to nitrogen status, is not regulated by methionine, glutamine or asparagine in isolation, and that it may respond to a glutamine metabolite.…”

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confidence: 99%

Soybean seeds overexpressing asparaginase exhibit reduced nitrogen concentration

Pandurangan

Pająk

Rintoul

et al. 2015

Physiologia Plantarum

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In soybean seed, a correlation has been observed between the concentration of free asparagine at mid-maturation and protein concentration at maturity. In this study, a Phaseolus vulgaris K -dependent asparaginase cDNA, PvAspG2, was expressed in transgenic soybean under the control of the embryo specific promoter of the β-subunit of β-conglycinin. Three lines were isolated having high expression of the transgene at the transcript, protein and enzyme activity levels at mid-maturation, with a 20- to 40-fold higher asparaginase activity in embryo than a control line expressing β-glucuronidase. Increased asparaginase activity was associated with a reduction in free asparagine levels as a percentage of total free amino acids, by 11-18%, and an increase in free aspartic acid levels, by 25-60%. Two of the lines had reduced nitrogen concentration in mature seed as determined by nitrogen analysis, by 9-13%. Their levels of extractible globulins were reduced by 11-30%. This was accompanied by an increase in oil concentration, by 5-8%. The lack of change in nitrogen concentration in the third transgenic line was correlated with an increase in free glutamic acid levels by approximately 40% at mid-maturation.

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Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro

Ochatt¹

2010

Methods in Molecular Biology

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Legumes are an important source of proteins and lipids for food and feed. In addition, they are -environmentally friendly because of their capacity to fix nitrogen through a symbiosis with Rhizobium that permits them to produce abundant proteins even in the absence of nitrogen fertilization. Seed development in plants follows three chronological steps (1) seed coat differentiation, embryo morphogenesis and endosperm development; (2) embryo maturation with storage accumulation and (3) dehydration and the acquisition of desiccation tolerance. Finally, germination occurs when the environmental conditions become favourable. Working with the model legume Medicago truncatula, an in vitro protocol was developed for the culture of immature embryos that permits their development in a way comparable to that observed in plants.In this chapter, the usefulness of this system for investigating embryo development in legumes is outlined.

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Effect of short-term application of nitrogen on the accumulation of β-subunit of β-conglycinin in nitrogen-starved soybean (Glycine maxL.) developing seeds

Cited by 9 publications

References 34 publications

Silencing of Soybean Seed Storage Proteins Results in a Rebalanced Protein Composition Preserving Seed Protein Content without Major Collateral Changes in the Metabolome and Transcriptome

Silencing of Soybean Seed Storage Proteins Results in a Rebalanced Protein Composition Preserving Seed Protein Content without Major Collateral Changes in the Metabolome and Transcriptome

Soybean seeds overexpressing asparaginase exhibit reduced nitrogen concentration

Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro

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