Effect of short-term versus prolonged freezing on freeze–thaw injury and post-thaw recovery in spinach: Importance in laboratory freeze–thaw protocols

Min, Kyungwon; Chen, Keting; Arora, Rajeev

doi:10.1016/j.envexpbot.2014.01.009

Cited by 44 publications

(38 citation statements)

References 64 publications

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“…Evidence abounds that plant tissues subjected to freeze–thaw accumulate excess

O_{2}^{\cdot -}

and H 2 O 2 (Kendall & McKersie, ; Min et al, ; Shin et al, ). Our data of visual detection of ROS (Figure ) are consistent with these findings and show that more severe freezing stress (−6.5˚C) resulted in higher ROS accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide (

O_{2}^{\cdot -}

) and hydrogen peroxide (H 2 O 2 ) distribution were visualized by nitroblue tetrazolium (NBT) and 3,3’‐diaminobenzidine (DAB) staining, respectively, using the protocol previously used in our laboratory for spinach (Chen & Arora, ; Min et al, ). Staining intensities were visually evaluated between F‐control and AsA‐fed leaves that were subjected to bath freezing at −5.5, −6.5, and −7.5°C.…”

Section: Methodsmentioning

confidence: 99%

“…Superoxide (O ⋅− 2 ) and hydrogen peroxide (H 2 O 2 ) distribution were visualized by nitroblue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB) staining, respectively, using the protocol previously used in our laboratory for spinach Min et al, 2014).…”

Section: Ros Stainingmentioning

confidence: 99%

“…Evidence abounds that plant tissues subjected to freeze-thaw accumulate excess O ⋅− 2 and H 2 O 2 (Kendall & McKersie, 1989;Min et al, 2014;Shin et al, 2018). Our data of visual detection of ROS (Figure 3) are consistent with these findings and show that more severe freezing stress (−6.5˚C) resulted in higher ROS ac- Fold changes in the concentrations of each metabolites between two groups (12 replications per treatment) were calculated using the formula log 2 (1.0 mM AsA/F-control); spot numbers and the numerical value of metabolites in this table are illustrated in Figure 5b.…”

Section: Higher Antioxidant Enzyme Activity In Asafed Leavesmentioning

confidence: 99%

“…physico-molecular perturbations in cell membranes, and (b) oxidative injury to macromolecules due to cellular accumulation of reactive oxygen species (ROS; e.g., superoxide, singlet oxygen, etc.) (Arora, 2018;Kendall & McKersie, 1989;Min, Chen, & Arora, 2014;Mittler, 2002). Hence, detoxification of excess ROS is believed to be one of the major strategies of frost survival (McKersie, Bowley, & Jones, 1999;McKersie et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

2020

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Freeze–thaw stress is one of the major environmental constraints that limit plant growth and reduce productivity and quality. Plants exhibit a variety of cellular dysfunctions following freeze–thaw stress, including accumulation of reactive oxygen species (ROS). This means that enhancement of antioxidant capacity by exogenous application of antioxidants could potentially be one of the strategies for improving freezing tolerance (FT) of plants. Exogenous application of ascorbic acid (AsA), as an antioxidant, has been shown to improve plant tolerance against abiotic stresses but its effect on FT has not been investigated. We evaluated the effect of AsA‐feeding on FT of spinach (Spinacia oleracea L.) at whole plant and excised‐leaf level, and conducted metabolite profiling of leaves before and after AsA treatment to explore metabolic explanation for change in FT. AsA application did not impede leaf growth, instead slightly promoted it. Temperature‐controlled freeze–thaw tests revealed AsA‐fed plants were more freezing tolerant as indicated by: (a) less visual damage/mortality; (b) lower ion leakage; and (c) less oxidative injury, lower abundance of free radicals (O2·- and H2O2). Comparative leaf metabolite profiling revealed clear separation of metabolic phenotypes for control versus AsA‐fed leaves. Specifically, AsA‐fed leaves had greater abundance of antioxidants (AsA, glutathione, alpha‐ & gamma‐tocopherol) and compatible solutes (proline, galactinol, and myo‐inositol). AsA‐fed leaves also had higher activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, and catalase). These changes, together, may improve FT via alleviating freeze‐induced oxidative stress as well as protecting membranes from freeze desiccation. Additionally, improved FT by AsA‐feeding may potentially include enhanced cell wall/lignin augmentation and bolstered secondary metabolism as indicated by diminished level of phenylalanine and increased abundance of branched amino acids, respectively.

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“…Evidence abounds that plant tissues subjected to freeze–thaw accumulate excess

O_{2}^{\cdot -}

Section: Discussionmentioning

confidence: 99%

“…Superoxide (

O_{2}^{\cdot -}

Section: Methodsmentioning

confidence: 99%

Section: Ros Stainingmentioning

confidence: 99%

Section: Higher Antioxidant Enzyme Activity In Asafed Leavesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

2020

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Transcriptome profiling of fully open flowers in a frost-tolerant almond genotype in response to freezing stress

et al. 2017

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Spring frost is a major limiting abiotic stress for the cultivation of almonds [Prunus dulcis (Mill.)] in Mediterranean areas or the Middle East. Spring frost, in particular, damages almond fully open flowers, resulting to significant reduction in yield. Little is known about the genetic factors expressed after frost stress in Prunus spp. as well as in almond fully open flowers. Here, we provide the molecular signature of pistils of fully open flowers from a frost-tolerant almond genotype. The level of frost tolerance in this genotype was determined for all three flowering stages and was confirmed by comparing it to two other cultivars using several physiological analyses. Afterwards, comprehensive expression profiling of genes expressed in fully open flowers was performed after being exposed to frost temperatures (during post-thaw period). Clean reads, 27,104,070 and 32,730,772, were obtained for non-frost-treated and frost-treated (FT) libraries, respectively. A total of 62.24 Mb was assembled, generating 50,896 unigenes and 66,906 transcripts. Therefore, 863 upregulated genes and 555 downregulated genes were identified in the FT library. Functional annotation showed that most of the upregulated genes were related to various biological processes involved in responding to abiotic stress. For the first time, a highly expressed cold-shock protein was identified in the reproductive organ of fruit trees. The expression of six genes was validated by RT-PCR. As the first comprehensive analysis of open flowers in a frost-tolerant almond genotype, this study represents a key step toward the molecular breeding of fruit tree species for frost tolerance.

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Impact of repeated freeze-thaw cycles environment on the allelopathic effect to Secale cereale L. seedlings

et al. 2022

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Effect of short-term versus prolonged freezing on freeze–thaw injury and post-thaw recovery in spinach: Importance in laboratory freeze–thaw protocols

Cited by 44 publications

References 64 publications

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

Transcriptome profiling of fully open flowers in a frost-tolerant almond genotype in response to freezing stress

Impact of repeated freeze-thaw cycles environment on the allelopathic effect to Secale cereale L. seedlings

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