Effect of Silicon dioxide coating of acrylic resin surfaces on Candida albicans adhesion

Abstract

Acrylic resin has been used in the manufacture of prostheses, however, in the oral cavity, this material starts to retain microorganisms capable of causing gingival inflammation due its porosities. The aim of this study was to evaluate the influence of the use of silicon dioxide as a coating layer applied onto acrylic resin, on the adhesion of Candida albicans (Ca). After the incubation period in Sabouraud Dextrose Broth, a total of 1 ml of the Ca suspension was added to plate wells, each well containing a specimen of acrylic resin. The adhesion ability of Ca on acrylic resin was determined by counting colonies. Three groups (n = 6) of acrylic resin were assessed: with polishing (RP); without polishing (RW); with polishing and coating layer of silicon dioxide (RPC). Ca deposited on the surface of the acrylic resin was also observed using Scanning Electron Microscopy (SEM). Statistical assessment by Kruskal-Wallis and Student-Newman-Keuls Method were done (α = 2%). There was significant difference among the groups. The RPC group showed the lowest growth, with an average of 5.59 Log CFU/cm²; there was a statistically significant difference in relation to group RW, which presented a growth of 6.07 Log CFU/cm² and to group RP with 5.91 Log CFU/cm 2 (p < 000.1). SEM images demonstrated that in the RP and RPC group, the surface of the resin had greater regularity, and smaller number of microorganisms. The application of silicon dioxide coating on acrylic resin appears to be a promising alternative, and its use can help in reducing the adhesion of Ca in prostheses.

Smart intraoral systems for advanced drug delivery

“…Furthermore, to prevent germ infiltration, surface modification might be a feasible strategy to fight against the sticky bioflim 157 . By way of illustration, polymer coatings, antimicrobial envelopes, and biosurfactants have proven to be highly potential to reduce or eliminate microbial adhesion on the biomaterials 158 . Moreover, enzyme‐mediated strategies, nanotechnologies, phage therapy, and immunotherapy emerge as promising approaches to compromise biofilm‐mediated infections due to deep penetration and precise actuation 159–161 .…”

“…157 By way of illustration, polymer coatings, antimicrobial envelopes, and biosurfactants have proven to be highly potential to reduce or eliminate microbial adhesion on the biomaterials. 158 Moreover, enzyme-mediated strategies, nanotechnologies, phage therapy, and immunotherapy emerge as promising approaches to compromise biofilmmediated infections due to deep penetration and precise actuation. [159][160][161] More importantly, combining traditional formulation with innovative technologies would also be an encouraging direction in the future.…”

Evaluation of biofilm formation on acrylic resin surfaces coated with silicon dioxide: an in situ study

“…The gauze was used to rub the product on the resin surface. 16 The blocks were stored in a vial for 24 h at room temperature to remove any solvent interferences and to wait for the curing time.…”

“…It has been demonstrated in a previous in vitro study that silicon dioxide coating on acrylic resin was able to reduce the adhesion of Candida albicans to prostheses. 16 As the objective of the present study was to evaluate whether silicon dioxide can inhibit the formation of microbial biofilm, only the total microbial load was evaluated, without the identification of microorganisms. It would be interesting to know which microorganisms were identified in the tested specimens and which microorganisms in the group treated with silicon dioxide showed significantly lower cell activity than the control.…”

“…13 Silicon dioxide coating results in a surface with a massively reduced amount of microorganisms and is easy to clean. In addition, silicon dioxide has been shown to be effective in reducing the adhesion of C. albicans to acrylic resin surfaces 14,15,16 and a recent in vivo study has demonstrated a high level of tissue biocompatibility of acrylic resin coated with silicon dioxide with low deleterious risk to patients. 17 Currently, it is possible to find it in the form of spray-on liquid glass, so it can be used easily in clinics.…”