Effect of sinapic acid on 1,2 dimethylhydrazine induced aberrant crypt foci, biotransforming bacterial enzymes and circulatory oxidative stress status in experimental rat colon carcinogenesis

Abstract

AIM: This study was aimed to investigate the effect of sinapic acid (SA) on 1,2-dimethylhydrazine (DMH) induced experimental rat colon carcinogenesis. MATERIALS AND METHODS: Rats were assorted into six groups, group 1 served as control, group 2 received SA (80 mg/kg b.w.) post orally every day until the end of the experimental period of 16 weeks, groups 3-6 rats were injected DMH (20 mg/kg b.w.) subcutaneously once a week for fi rst four weeks. In addition, groups 4-6 rats received different doses of SA (20, 40 and 80 mg/kg b.w.). RESULTS: Our results showed that DMH induced rats revealed signifi cantly increased ACF development and multiplicity, which were signifi cantly inhibited on supplementation with SA. Moreover, elevated levels/activities of circulatory oxidative stress markers, faecal and colonic mucosal bacterial enzymes were observed in DMH exposed rats, which were diminished on supplementation with SA. CONCLUSION: Overall, our fi ndings revealed that supplementation with SA offers signifi cant protection against DMH induced rat colon carcinogenesis and the effect of SA at the dose of 40 mg/kg b.w. was more pronounced as compared to the other two doses (Tab. 5, Fig. 3, Ref. 46). Text in PDF www.elis.sk.

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

“…considered a primary causative factor in colon cancer, via the formation of methylazoxymethanol, a metabolite that induces the formation of DNA adducts. SA was effective in reducing the development of pre-cancerous lesions when studied previously (Balaji et al, 2015).…”

“…SA also inhibited carcinogenesis by increasing the activities of enzymatic and non-enzymatic antioxidants, including SOD, catalase (CAT) and GSH, with ROS known to play a role in early carcinogenesis. SA could potentially prevent the onset of carcinogenesis, as demonstrated in a study by Balaji et al (2015). Colon cancer is the third most common malignancy worldwide, with a high-fat diet being a primary risk factor for developing colon cancer.…”

“…Research on these phenolic compounds has suggested that they possess diverse bioactive properties, including anti-cancer, anti-diabetes and anti-inflammatory activities, in addition to a role in the prevention and treatment of cardiovascular and neurodegenerative diseases (Yun et al, 2008;Balaji et al, 2015;Ju et al, 2015;Semaming et al, 2016;Talagavadi et al, 2016). Given these potent phosphatidylinositol 3-kinase/Akt (IRS-1/PI3K/Akt) signalling pathway, leading to increased glucose uptake.…”

Mechanisms involved in the anticancer effects of sinapic acid

“…SA treatment reduced the incidence of polyp up to 66.66% and prevented the DMH-induced histological abnormalities such as dysplasia, enlarged nuclei and densely packed inflammatory cell infiltrates and lymphoid aggregates in colon. In another study (Balaji et al 2015) on colon cancer, oral administration of SA up to 80 mg/kg body weight reduced the number of aberrant crypt foci up to 34.55% compared to the DMH-induced colon cancer-induced group (58.83%). Anti-tumor effect of SA on dimethyl benz(a)anthracene (DMBA)-induced experimental oral cancer was reported in Syrian hamsters (Kalaimathi and Suresh 2015a).…”

Grain Legume Consumption Inhibits Colorectal Tumorigenesis: A Meta-Analysis of Human and Animal Studies

“…Proanthocyanidins can inhibit ACF formation at concentrations of 0.002-1% of the diet or by gavage [145][146][147]. Phenolic acids include ferulic acid (~0.003% of grain legume dry weight) that inhibited ACF formation at concentrations of 0.25-1% [148][149][150] and sinapic acid that inhibited ACF formation at concentrations of 20-80 mg/kg of body weight by gavage [151]. The concentrations of the phyto-estrogen group's isoflavonoids (0.005-0.095 mg/kg grain legume) and lignans (0.018-0.266 mg/kg grain legume) are relatively low in grain legumes [152] and, thus, probably contribute little to the chemo-preventive effect of grain legumes.…”