Effect of single-dose ibuprofen on follicular fluid levels of interleukins in poor responders undergoing<i>in vitro</i>fertilization

Nemer, Laurice Bou; Shi, Haolin; Carr, Bruce R.; Word, R. Ann; Bükülmez, Orhan

doi:10.1080/19396368.2018.1557761

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…[ 44 – 50 ]IL-6 is a multifunctional cytokine, and the role of IL-6 in human oocyte maturation and subsequent embryo development is still uncertain. Some studies have suggested that high levels of IL-6 in follicular fluid are beneficial for oocyte maturation [ 42 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have suggested that high levels of IL-6 in follicular fluid are beneficial for oocyte maturation [ 42 , 52 ]. However, other studies have drawn opposite conclusions: higher levels of IL-6 during in vitro fertilization cycles are associated with poorer embryo quality and lower chances of pregnancy [ 43 , 44 ]. There are also studies suggesting that IL-6 does not affect the clinical pregnancy rate in IVF-ET and therefore does not affect oocyte quality [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

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Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy

Hu,

Zheng,

Zhang

2024

J Ovarian Res

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Background The ovarian environment of premature ovarian insufficiency (POI) patients exhibits immune dysregulation, which leads to excessive secretion of numerous proinflammatory cytokines that affect ovarian function. An abnormal level of macrophage polarization directly or indirectly inhibits the differentiation of ovarian granulosa cells and steroid hormone production, ultimately leading to POI. Resveratrol, as a health supplement, has been widely recognized for its safety. There is a substantial amount of evidence indicating that resveratrol and its analogs possess significant immune-regulatory functions. It has also been reported that resveratrol can effectively inhibit the progression of POI. However, the underlying immunological and molecular mechanisms through which resveratrol inhibits the progression of POI are still unclear. Results Our preliminary reports have shown that resveratrol-βcd, the beta-cyclodextrin complex of resveratrol, significantly enhances the stability of resveratrol. Resveratrol-βcd could regulate the dysfunctional immune status of macrophages and T cells in the tumor microenvironment. In this study, we treated busulfan and cyclophosphamide (B/C)-treated mice, which were used as a POI model, with resveratrol-βcd. After resveratrol-βcd treatment, the levels of IL-6 in the ovaries were significantly increased, and the progression of POI was suppressed. IL-6 activated granulosa cells (GCs) through soluble IL-6R (sIL-6R), promoting autophagy in GCs. Resveratrol-βcd and IL-6 had a synergistic effect on enhancing autophagy in GCs and promoting E2 secretion. Conclusions We partially elucidated the immune mechanism by which resveratrol inhibits the progression of POI and the autophagy-regulating function of GCs. This provides a theoretical basis for using resveratrol to prevent POI in future studies and clinical guidance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy

Hu,

Zheng,

Zhang

2024

J Ovarian Res

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“…Elevated IL6 levels have been associated with improved clinical pregnancies by enhancing embryo implantation [60,61]. Conversely, elevated IL6 levels have been linked to diminished embryo quality and decreased likelihood of successful pregnancies [62]. In swine, IL6 receptors have been identified as promoters of granulosa cell survival [63].…”

Section: Interleukin-6 (Il6)mentioning

confidence: 99%

Cytokine Profiling and Puberty Enhancement Post Altrenogest Feeding in Pre Pubertal Murrah Buffalo (<em>Bubalus bubalis</em>) Heifers

Singh,

Ratta

et al. 2024

Preprint

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Cytokine and AMH profiling were done in prepubertal Murrah buffalo heifers post-progesterone supplementation and co-synchronization protocol. Cytokines: IFNγ, IL6, IL1, IL13, TNFα, and TGFβ, and, AMH, Progesterone and Estrogen hormone estimations were done. Ovarian follicular pattern and fertility outcome were recorded. Eighteen prepubertal heifers, 15-17 months of age, 250-300 kg BWt were randomly divided into 3 groups, Group1: n=6, supplemented with altrenogest 0.044mg/kg/day/heifer orally for 14 days along with Co-synch programme; Group2: n=6, administered CIDR for 14 days and co-synch programme, Group3: n=6, received co-synch programme. Ultrasonography was done to ovarian follicle status on respective days of sampling. Pregnancy diagnosis was done around 45 and 60 post-AI. Estradiol 17-β level remained constant in altrenogest group, CIDR implanted and Control groups during the Progesterone treatment and Co-synch treatment. Estradiol levels during Co-synch were significantly less on day 9 of Co-synch (14.41 ± 1.97 pg/mL) than day 0 and on day7 in pre pubertal buffalo heifers in altrenogest group. However, no significance was observed in other groups. Progesterone levels in buffalo heifers subjected to synchronisation protocols varied significantly (P< 0.05) on day 7 of P4 treatment between altrenogest supplemented and CIDR implanted groups, as well as, Co-synch groups. Supplementation of altrenogest showed a significant (P= 0.043) increase in Progesterone levels by day 14 of altrenogest treatment. Progesterone varied significantly in all Groups on day 9 of co synch protocol [ Group 1 (P= 0.02), Group 2 (P= 0.041) and Group 3 (P= 0.007)]. Cytokine IFN-γ showed high correlation with progesterone, indicating the role of IFN-γ in puberty in buffalo heifers (r= 0.626, P< 0.01). AMH had a significant positive correlation supplemented with altrenogest with IFN-γ (r= 0.673, P< 0.01) and TGF-β (r= 0.463, P< 0.01), whereas, with TNF-α it was a negatively correlated (r= -0.34, P< 0.05). Based on Karl Pearson correlation coefficients, IL-13 and TGF-β could be considered as markers for puberty in buffalo heifers.

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“…Higher levels of IL-6 correlate with poor embryo quality, and patients are less likely to become pregnant. [17][18][19] It has been concluded that IL-6 inhibits oocyte development. [20] In addition, some studies have suggested that IL-6 does not affect the clinical rate of pregnancy, as well as the quality of oocytes.…”

Section: Introductionmentioning

confidence: 99%

IL-6 of follicular fluid and outcome of in vitro fertilization

et al. 2022

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The quality of an oocyte is influenced by its microenvironment, which includes cumulus cells and follicular fluid, as well as cells of the immune system and their products. The ovarian interleukins, which are secreted by the granulosa cells and other immune cells within the ovaries and follicles, regulate various functions between the cells. IL-6 is a cytokine that is present in the follicular fluid and may affect the quality of oocytes. There are some inconsistencies in the literature regarding the concentration of interleukin 6 in the follicular fluid. The main objective of this study was to examine whether the concentration of interleukin 6 in the follicular fluid affects the outcome of IVF. This study involved 83 patients who underwent IVF. Follicular fluid was used as the biological material for the analysis. Examination of the obtained follicular fluid and collection of oocytes under a stereomicroscope was performed in the embryological laboratory. The concentration of IL-6 in the follicular fluid was analyzed. IVF and ICSI methods were used as the fertilization methods. Pregnancy was confirmed by the positive serum β-hCG level. The software package SPSS 20 was used for statistical data processing. Analysis of the follicular fluid samples showed a correlation between the concentration of IL-6 in the follicular fluid and the outcome of IVF. The concentration of IL-6 in the follicular fluid was higher in patients with confirmed pregnancy (9.55 ± 7.47 ng/ml). Based on our results, we conclude that the concentration of IL-6 affects the outcome of IVF. If the range of IL-6 concentration is between 3,67 ng/ml and 10 ng/ml, we can expect good IVF outcome with vital pregnancy.

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Effect of single-dose ibuprofen on follicular fluid levels of interleukins in poor responders undergoingin vitrofertilization

Cited by 9 publications

References 36 publications

Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy

Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy

Cytokine Profiling and Puberty Enhancement Post Altrenogest Feeding in Pre Pubertal Murrah Buffalo (<em>Bubalus bubalis</em>) Heifers

IL-6 of follicular fluid and outcome of in vitro fertilization

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