Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses

Ortiz, Isabel; Dorado, J.; Ramirez, L.; Morrell, Jane M.; Acha, D.; Urbano, M.; Gálvez, Milagros Jiménez; Carrasco, J.J.; Gómez-Arrones, V.; Calero-Carretero, R.; Hidalgo, M.

doi:10.1017/s1751731113002097

Cited by 21 publications

(16 citation statements)

References 37 publications

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“…These results are in agreement with previous studies where sperm parameters were improved after colloid centrifugation in donkey semen using Androcoll-E-Large [18] and Equipure [19]. Sperm quality is considered a parameter of crucial importance in assisted reproduction.…”

Section: Discussionsupporting

confidence: 92%

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

et al. 2015

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Section: Discussionsupporting

confidence: 92%

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

et al. 2015

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“…Total (TM, %) and progressive (PM, %) sperm motility were evaluated by computer-assisted sperm analysis (CASA) using the Sperm Class Analyzer (SCA 2011 v.5.0.1; Microptic S.L., Barcelona, Spain) with the settings described by Ortiz et al [13]. Membrane integrity was assessed using Vital Test kit (Halotech DNA, Madrid, Spain) following the manufacturer’s instructions [10].…”

Section: Methodsmentioning

confidence: 99%

New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models

Ortiz

Dorado

Morrell

et al. 2017

J Animal Sci Biotechnol

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BackgroundSperm DNA fragmentation (sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if sDF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model (linear regression, exponential or polynomial) for DNA fragmentation over time in frozen-thawed donkey semen.ResultsAfter submitting post-thaw semen samples to no centrifugation (UDC), sperm washing (SW) or single layer centrifugation (SLC) protocols, sDF values after 6 h of incubation were significantly lower in SLC samples than in SW or UDC. Coefficient of determination (R2) values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation (aSDF) were obtained for SW, followed by SLC and UDC.ConclusionSLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover, the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.

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“…La congelación del semen se relaciona con daños en la membrana plasmática, las mitocondrias y otras organelas de los espermatozoides (Ortiz et al, 2014;Darr et al, 2016). Esto se ve reflejado en la reducción de la movilidad, la viabilidad y la capacidad fecundante de dichas células (Najafi et al 2014).…”

Section: Introductionunclassified

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

2017

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Palabras clave adicionalesCalidad seminal. Crioprotectores. Plasma seminal. Yema de huevo. Criopreservación. resUMenLa congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de suplementación de un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) y luego se congeló en diluyente Equiplus® suplementado con 3% de glicerol (GLY) ó 5% de dimetilformamida (DMF), 2% ó 5% de yema de huevo centrifugada (YHC) y 10% ó 20% de plasma seminal (PS). Se realizó la evaluación post-descongelación de la calidad seminal y de la peroxidación lipídica (PL). Para el análisis estadístico se ajustaron modelos mixtos, se realizó un análisis de regresión y comparaciones de medias por la prueba de Tukey. El semen congelado con 5% de DMF, 5% de YHC y 10% de PS presentó valores superiores de movilidad, vitalidad e integridad de membrana (p<0,05). La PL fue menor en diluyentes con 5% de YHC (p<0,05). La suplementación del diluyente de congelación con una combinación de 5% de DMF, 5% de YHC y 10% de PS permite mejorar la calidad post-descongelación del semen de asno. Effect of extender supplementation on the post-thaw semen quality of donkey sUMMarY Freezing of donkey semen allows storing genetic material for an unlimited period of time, which can be used to generate germoplasm banks or for breeding of mules or asses. This research aimed to evaluate supplementation alternatives for freezing donkey semen. Semen from five Colombian creole donkeys (n= 10) was collected and then frozen in Equiplus® extender supplemented with 3% of glycerol (GLY) or 5% of dimethylformamide (DMF), 2% or 5% of centrifuged egg yolk (CEY) and 10% or 20% of seminal plasma (SP). Post-thaw evaluation of semen quality and lipid peroxidation (LP) was conducted. For statistical analysis mixed models were adjusted, regression analysis and comparison of means by the Tukey test were performed. Frozen semen with DMF, 5% CEY and 10% SP presented superior results of motility and membrane integrity (p<0.05). The LP was lower in extenders with 5% CEY (p<0.05). Supplementation of freezing extender with the combination of 5% DMF, 5% CEY and 10% SP allows to improve the post-thaw quality of asinine semen.additional keYwords

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Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses

Cited by 21 publications

References 37 publications

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

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