Effect of sinomenine on cytokine expression of macrophages and synoviocytes in adjuvant arthritis rats

Wang, Yong; Fang, Yongfei; Huang, Weixue; Zhou, Xin; Wang, Ming-Hai; Zhong, Bing; Peng, Dan

doi:10.1016/j.jep.2004.12.022

Cited by 115 publications

(72 citation statements)

References 23 publications

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“…8,20) Previous mechanistic studies revealed that SIN could protect the arthritic joints by decreasing mRNA expression of TNF-a and IL-1b, inhibiting production of prostaglandin E2 (PGE2) and nitric oxide (NO), and so on. 8,21) Much of the eŠects of SIN are related to the etiological factors of OA, but up to now, little is known whether SIN has a protective eŠect on OA. In the present study, we demonstrated the obvious eŠectiveness of SIN in protecting rabbit cartilage and chondrocyte against cartilage degradation and chondrocyte apoptosis in an IL-1b-mediated OA model.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis

Deng

et al. 2010

YAKUGAKU ZASSHI

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Sinomenine (SIN), an alkaloid extracted from the stem of the Chinese medicinal plant sinomenium acutum, has been used for treating rheumatoid arthritis. But little is known whether SIN has a protective eŠect on osteoarthritis (OA). In this study, we investigated the protective eŠect of SIN on IL-1b-induced proteoglycan degradation and apoptosis in rabbit articular cartilage and chondrocytes. Treatment with 10 ng/ml IL-1b increased the level of glycosaminoglycan (GAG) released into the culture media, and up-regulated the activity and mRNA expression of matrix metalloproteinase 13 (MMP-13) and down-regulated the activity and mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) in cartilage explants, as conˆrmed by the methods of GAG quantitation, MMP-13/TIMP-1 enzyme-linked immunosorbent assay (ELISA) and real-time quantitative RT-PCR. Treatment with 10 ng/ml IL-1b resulted in marked apoptosis in chondrocytes, as demonstrated by decreased cell viability, occurrence of DNA laddering and increased caspase-3 activity and annexin V binding of phosphatidylserine. However, simultaneous treatment with SIN (10, 50 or 250 mM) inhibited the GAG release and the activity and mRNA expression of MMP-13, and enhanced the activity and mRNA expression of TIMP-1 in a dose-dependent manner in cartilage explants. Furthermore, DNA fragment, caspase-3 activity and apoptosis rate were down-regulated, and cell viability was up-regulated dose-dependently in chondrocytes. Thus, SIN has the protective capacity to antagonize cartilage degradation and chondrocyte apoptosis, which suggest that SIN may act as an agent for pharmacological intervention in the progress of OA.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis

Deng

et al. 2010

YAKUGAKU ZASSHI

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“…After the rat is immunized by injection of Freund's complete adjuvant into the left hind foot pad, the animals develops the primary and acute inflammation in the joint of injected side during 1~5 days. The secondary and chronic inflammation occurs in the contralateral (non-injected) legs and becomes progressively severe during 7~28 days with peak response on day 21 [9,21,22] . Our data showed that oral administration of AST (100 mg/kg/d) significantly reduced both acute and chronic inflammation in AIA rats [9] .…”

Section: Ast Suppresses Joint Inflammation In Rat Adjuvantinduced Artmentioning

confidence: 99%

Anti-arthritic effect of astragaloside IV and its molecular mechanism

Wang

2014

Inflamm Cell Signal

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The anti-inflammatory drugs and biological agents have been commonly used for the treatment of rheumatoid arthritis (RA) and other chronic inflammatory joint diseases. However, the prolonged use of such drugs may lead to severe side effects. Therefore, there is a growing need for newer drugs that are effective and less toxic for the treatment of these diseases. Natural plant extracts and purified bioactive compounds offer a promising resource for potential anti-arthritic agents. The saponin astragaloside IV (AST) is one of major active components purified from Astragalus membranaceus, which has been widely used in traditional Chinese medicine to treat immune disorders including RA. This review summarizes our recent findings on antiarthritic effect of AST and its molecular mechanism.

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“…Sinomenine (SIN,7, C 19 H 23 NO 4 ), an alkaloid isolated from the Chinese medicinal plant, Sinomenium acutum, has been utilized to safely treat arthritis for many years. Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.…”

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confidence: 99%

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Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

Chen

et al. 2009

Acta Pharmacol Sin

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Aim:The aim of this study was to investigate the mechanism of the effects of Sinomenine (SIN) on the invasion and migration ability of activated human monocytic THP-1 cells (A-THP-1). Sinomenine is a pure alkaloid extracted from the Chinese medical plant Sinomenium acutum. Methods: Human monocytic THP-1 cells were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were treated with different concentrations of SIN. The invasion and migration ability of cells was tested by in vitro transwell assays. The levels of CD147 and MMPs were evaluated by flow cytometric analysis and zymographic analysis, respectively. The mRNA expression of CD147, MMP-2, and MMP-9 was measured by RT-PCR. Results:The invasion and migration ability of A-THP-1 cells was significantly inhibited by SIN in a concentration-dependent fashion; at the same time, the levels of CD147, MMP-2, and MMP-9 were markedly down-regulated. This inhibitory effect was most notable at concentrations of 0.25 mmol/L and 1.00 mmol/L (P<0.01). Conclusion: A possible mechanism of the inhibitory effect of SIN on cell invasion and migration ability is repression of the expression of MMP-2 and MMP-9, which strongly correlates with the inhibition of CD147 activity. IntroductionSinomenine (SIN,7, C 19 H 23 NO 4 ), an alkaloid isolated from the Chinese medicinal plant, Sinomenium acutum, has been utilized to safely treat arthritis for many years. Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.Enhanced migration and invasion of peripheral macrophages contribute to joint destruction in RA by directly degrading the cartilage matrix and indirectly promoting angiogenesis. Elevated gene expression of gelatinase A (also called matrix metalloproteinase-2, MMP-2) and gelatinase B (also called matrix metalloproteinase-9, MMP-9) is critical for the progression of RA [8] .The extracellular matrix metalloproteinase inducer (EMMPRIN, CD147) is a heavily glycosylated protein containing two immunoglobulin super family domains. It is enriched on the surface of macrophages and is associated with differentiation of human monocytic THP-1 cells when treated with phorbol 12-myristate 13-acetate (PMA). This protein stimulates the production of matrix metalloproteinases (MMPs). CD147 has also been reported to play an important role in the invasion and migration ability of cells in both animal models and cancer patients [9] .Although many in vivo studies have demonstrated that SIN can significantly improve...

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Effect of sinomenine on cytokine expression of macrophages and synoviocytes in adjuvant arthritis rats

Cited by 115 publications

References 23 publications

Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis

Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis

Anti-arthritic effect of astragaloside IV and its molecular mechanism

Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

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