Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

Li, X.J.; Yue, Patrick; Ha, W. Y.; Wong, Daisy Y.L.; Tin, Mandy M.Y.; Wang, P.X.; Wong, Ricky N S; Liu, L.

doi:10.1016/j.lfs.2006.02.014

Cited by 39 publications

(39 citation statements)

References 24 publications

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“…Sinomenine has attracted great attention for its anti-neoplasm potential. It has been demonstrated to inhibit cell proliferation and induce apoptosis in a variety of human tumor cells (11)(12)(13)(14). Li et al (15) reported that sinomenine induces vasculature normalization that contributes to antitumor and anti-metastasis effect on breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Xie

Ren

Lin

et al. 2016

International Journal of Oncology

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Abstract. Osteosarcoma is the most common primary malignant tumor of the bone. The long-term survivals continue to be unsatisfactory for patients with metastatic and recurrent disease. Metastasis is still a severe challenge in osteosarcoma treatment. Sinomenine, an alkaloid from traditional Chinese medicine, has been proved to possess potent antitumor and anti-invasion effect on various cancers. However, the effect of sinomenine on human osteosarcoma and the underlying mechanisms remains unknown. We report here that sinomenine inhibited proliferation by inducing S phase arrest and suppressing the clone formation. Significant inhibitory effects were found in invasion and metastasis in osteosarcoma, but little cytotoxicity was observed in tested concentrations. Exposure to sinomenine resulted in suppression of invasion and migration in osteosarcoma cells as well as tube formation ability in the human umbilical vein endothelial cells (HUVEC) and U 2 OS cells. Furthermore, it demonstrated that CXCR4 played a key role contributing to invasion in osteosarcoma which is considered to be a core target site in sinomenine treatment. Sinomenine inhibited invasion by suppressing CXCR4 and STAT3 phosphorylation then downregulating the expression of MMP-2, MMP-9, RANKL, VEGF downstream. In addition, then RANKL-mediated bone destruction stimulated by osteoclastogenesis and VEGF-related neovascularization were restrained. Importantly, in vivo, sinomenine suppressed proliferation, osteoclastogenesis and bone destruction. Through these various comprehensive means, sinomenine inhibits metastasis in osteosarcoma. Taken together, our results revealed that sinomenine caused S phase arrest, inhibited invasion and metastasis via suppressing the CXCR4-STAT3 pathway and then osteoclastogenesis-mediated bone destruction and neovascularization in osteosarcoma. Sinomenine is therefore a promising adjuvant agent for metastasis control in osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Xie

Ren

Lin

et al. 2016

International Journal of Oncology

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“…Tel: 0086-571-87236346. Sinomenine (SN) is an immunosuppressive compound derived from the Chinese medicinal plant, Sinomenium acutum, and has been successfully applied to the treatment of various autoimmune diseases (Park et al, 2007). Previous studies have also indicated that SN can inhibit lymphocyte proliferation, production of pro-inflammatory factors by macrophage, and NF-κB activity Li et al, 2006;Shu et al, 2007). Although, in vitro models of leukemia treated with anti-inflammatory drugs have been shown to induce apoptosis (Subhashini et al, 2005) little is known about the mechanism by which SN may induce the apoptosis of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Sinomenine induces apoptosis of prostate cancer cells by blocking activation of NF-kappa B

Fan¹,

Wang²,

Chen³

et al. 2011

Afr. J. Biotechnol.

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Sinomenine (SN) is an alkaloid that has previously been shown to have a role in such varied processes as inflammation, angiogenesis, arthritis and immunosuppression. Therefore, in this study, possible anti-tumor effects of SN on the human prostate cancer cell lines, PC-3 and DU-145, was investigated. After treatment with varying doses of SN, apoptosis was measured by flow cytometry. Prostaglandin E2 production was detected by ELISA, and expression levels of cyclooxygenase-2 and nuclear factorkappa B were evaluated by western blot. In both PC-3 and DU-145 cells, SN treatment induced apoptosis, inhibited in vitro production of prostaglandin E2, and decreased activation of cyclooxygenase-2 and nuclear factor-kappa B. Growth inhibition assays were also performed to determine whether cell viability was responsible for the decrease in the activation observed. In combination, these results suggest that SN can induce an anti-tumor response by suppressing the activation of cyclooxygenase-2 and nuclear factor-kappa B signaling pathways.

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Section: Introductionmentioning

confidence: 99%

“…Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.…”

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confidence: 99%

Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

Chen

et al. 2009

Acta Pharmacol Sin

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Aim:The aim of this study was to investigate the mechanism of the effects of Sinomenine (SIN) on the invasion and migration ability of activated human monocytic THP-1 cells (A-THP-1). Sinomenine is a pure alkaloid extracted from the Chinese medical plant Sinomenium acutum. Methods: Human monocytic THP-1 cells were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were treated with different concentrations of SIN. The invasion and migration ability of cells was tested by in vitro transwell assays. The levels of CD147 and MMPs were evaluated by flow cytometric analysis and zymographic analysis, respectively. The mRNA expression of CD147, MMP-2, and MMP-9 was measured by RT-PCR. Results:The invasion and migration ability of A-THP-1 cells was significantly inhibited by SIN in a concentration-dependent fashion; at the same time, the levels of CD147, MMP-2, and MMP-9 were markedly down-regulated. This inhibitory effect was most notable at concentrations of 0.25 mmol/L and 1.00 mmol/L (P<0.01). Conclusion: A possible mechanism of the inhibitory effect of SIN on cell invasion and migration ability is repression of the expression of MMP-2 and MMP-9, which strongly correlates with the inhibition of CD147 activity. IntroductionSinomenine (SIN,7, C 19 H 23 NO 4 ), an alkaloid isolated from the Chinese medicinal plant, Sinomenium acutum, has been utilized to safely treat arthritis for many years. Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.Enhanced migration and invasion of peripheral macrophages contribute to joint destruction in RA by directly degrading the cartilage matrix and indirectly promoting angiogenesis. Elevated gene expression of gelatinase A (also called matrix metalloproteinase-2, MMP-2) and gelatinase B (also called matrix metalloproteinase-9, MMP-9) is critical for the progression of RA [8] .The extracellular matrix metalloproteinase inducer (EMMPRIN, CD147) is a heavily glycosylated protein containing two immunoglobulin super family domains. It is enriched on the surface of macrophages and is associated with differentiation of human monocytic THP-1 cells when treated with phorbol 12-myristate 13-acetate (PMA). This protein stimulates the production of matrix metalloproteinases (MMPs). CD147 has also been reported to play an important role in the invasion and migration ability of cells in both animal models and cancer patients [9] .Although many in vivo studies have demonstrated that SIN can significantly improve...

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Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

Cited by 39 publications

References 24 publications

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Sinomenine induces apoptosis of prostate cancer cells by blocking activation of NF-kappa B

Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

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