Effect of siRNA-mediated downregulation of VEGF in Tca8113 cells on the activity of monocyte-derived dendritic cells

Ni, Yanhong; Wang, Zhiyong; Huang, Xiaofeng; Shi, Peihua; Han, Wei; Hou, Yayi; Zhang, Hua; Hu, And Qin-Gang

doi:10.3892/ol.2012.568

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Abnormally high blood concentrations of VEGF have been shown to be associated with poor prognosis in solid as well as hematological malignancies ( 24 ). Inhibition of angiogenesis may be one of the mechanism through which the activation of effective anticancer immunity controls neoplastic growth ( 25 – 28 ).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells

Wang

Zhang²,

Zhang

et al. 2014

Experimental and Therapeutic Medicine

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This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) secreted by MCF-7 breast cancer cells on the differentiation, maturation and function of dendritic cells (DCs). Small interfering RNAs (siRNAs) directed against the VEGF gene were designed and transfected into MCF-7 breast cancer cells at an optimal concentration (100 nmol/l) using cationic liposome transfection reagent, whereas the control group was transfected with only transfection reagent. Western blot analysis and ELISA were used to determine VEGF protein expression and VEGF concentration, respectively. Mononuclear cells were cultured with the culture supernatants from primary MCF-7 cells (control group) and siRNA-treated MCF-7 cells (siRNA group). The DC phenotypes, including CD1a, CD80, CD83, CD86 and HLA-DR, were evaluated by flow cytometry. The MTT assay was used to assess the cytotoxicity of DC-mediated tumor-specific cytotoxic T lymphocytes (CTLs) against MCF-7 cells in the two different culture supernatants. The VEGF-targeted constructed siRNA inhibited VEGF expression in MCF-7 cells. Cultivation with the culture supernatants from MCF-7 cells treated with siRNA affected DC morphology. DCs in the siRNA group exhibited a significantly higher expression of CD86, CD80, CD83 and HLA-DR compared to the cells in the control group, whereas the expression of CD1a in the siRNA group was significantly lower compared to that in the control group. The cytotoxic activity of CTLs mediated by DCs was significantly altered by siRNA transfection. These results indicated that VEGF may play a significant role in tumor development, progression and immunosuppression.

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Section: Introductionmentioning

confidence: 99%

Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells

Wang

Zhang²,

Zhang

et al. 2014

Experimental and Therapeutic Medicine

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Vascular endothelial growth factor suppresses dendritic cells function of human prostate cancer

Bai¹,

Zhang²,

Hu³

2018

OTT

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PurposeProstate cancer (PCa) patients often have dendritic cell (DC) function defects, but the mechanism is not clear. The aim of this study was to detect the effect of vascular endothelial growth factor (VEGF) in mature DCs.Patients and methodsIn this study, we chose 30 PCa patients, 10 prostatic intraepithelial neoplasia (PIN) patients and 30 benign prostatic hyperplasia (BPH) patients, and compared the composition of peripheral blood T cells, the composition and function of local dendritic cells in prostate tissue, and the density of local VEGF.ResultsThe results showed that the numbers of total DCs, mature and functional DCs, and CD4+ T cells were inhibited in PCa, and the inhibitory effect was enhanced with increased malignancy. In addition, the infiltration density of VEGF-positive cells was increased in PCa, and this increase was associated with an increased malignant degree of PCa. The inhibition of tumor immunity in patients with PCa is achieved by inhibiting the function of dendritic cells.ConclusionVEGF plays an important role in the inhibition of the maturation and function of dendritic cells, and this inhibition is gradually increased with an increasing malignant degree of PCa.

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Impact of siRNA transfection on tumor development under experimental pathology of thyroid gland

et al. 2013

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Effect of siRNA-mediated downregulation of VEGF in Tca8113 cells on the activity of monocyte-derived dendritic cells

Cited by 3 publications

References 20 publications

Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells

Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells

Vascular endothelial growth factor suppresses dendritic cells function of human prostate cancer

Impact of siRNA transfection on tumor development under experimental pathology of thyroid gland

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