Effect of Skim Milk and Tris-citrate Extenders to Preserve the Semen of Indigenous Ram of Bangladesh

Rahman, M. S.; Gofur, M. R.; Rahman, M. M.; Bari, Farida Yeasmin; Juyena, Nasrin Sultana

doi:10.9734/ajob/2018/39448

Cited by 17 publications

(9 citation statements)

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“…En este sentido, los resultados varían desde el 20% al 60% (Memon et al,. 2013;Rahman et al, 2018;Tekin y Daskin, 2016;Vidal et al, 2013), la diferencia podría estar dada precisamente en la presencia o no del plasma seminal.…”

Section: Resultados Y Discusiónunclassified

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

Duran¹,

Tellez²,

Martinez³

et al. 2022

Trop Subtrop Agroecosyst

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<pre>Background: Prior to freezing goat semen, it is necessary to perform seminal washing by centrifugation to eliminate Phospholipase A, with the consequent loss of elements involved in maintaining sperm functions. Objective: Determine the adequate concentration of egg yolk (YH) for freezing goat semen in a lyophilized diluent based on Tris-glucose and citric acid, without performing seminal washing by centrifugation. Methodology: ninety ejaculates were evaluated with 12 replicates, collected twice a week by means of Artificial Vagina. Volume, motility, concentration, viability and total pathologies were measured. The fit ejaculates were united and divided into five portions, each one received the corresponding diluent: Tris-glucose-Ac. Citrus with YH (2.25%, 3.37%, 4.45% and 5.65%) and the control diluent containing lactose-skimmed milk (DC). The final sperm concentration in the samples was 1.5 x 109 mL</pre>-1. The equilibrium period was carried out at 5°C for 2 h. Subsequently, it was frozen in 0.1 mL tablets in nitrogen vapors, and stored for 7 d in liquid nitrogen, thawed at 37°C and the percentages of motility (30 min, 120 min and 240 min), viability and total pathologies (30 min and 120 min) were determined. The diluents were compared using a Binary Logistic Regression model. Results: YH (4.45%) and DC had the highest probability (P <0.05) of motility at all times. The highest probability (P <0.05) of viability was for YH (4.45%), and the lowest probability (P <0.05) of total pathologies for 4.45% YH and DC, at 30 min and 120 min. Implications: In the freezing of goat semen, it is possible to eliminate the seminal washing process by centrifugation. Conclusion: Goat semen can be frozen in a Tris-based lyophilized extender with 4.45% egg yolk, without performing seminal washing by centrifugation.

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Section: Resultados Y Discusiónunclassified

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

Duran¹,

Tellez²,

Martinez³

et al. 2022

Trop Subtrop Agroecosyst

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“…When stored at 5°C with SM-EY extender, sperm progressively motility was maintained ≥40% for five days in T1 and three days in T0 (control) and T2. The control group outperformed refrigerator-preserved indigenous ram semen, which maintained the minimum motility for only 48 h in skim milk extender [ 54 ]. However, the control group in this study was inferior to Ettawa goat semen in skim milk extender, which maintained the threshold of progressive motility for four days at 5°C [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Susilowati

Mustofa

Wurlina

et al. 2021

Veterinary Medicine International

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Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

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“…Skim milk Available at www.veterinaryworld.org/Vol.14/May-2021/21.pdf proteins buffer semen pH and may also chelate any heavy metal ions [94]. An important milk compound is lactose, which is hydrophilic [95] and cannot diffuse the cell wall of the sperm cells, which protects the cell wall and prevents freeze shock [94][95][96]. Skim milk-based extender is superior to Tris-based extender based on semen preservation [96].…”

Section: Milk Sourcesmentioning

confidence: 99%

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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Effect of Skim Milk and Tris-citrate Extenders to Preserve the Semen of Indigenous Ram of Bangladesh

Cited by 17 publications

References 0 publications

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

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