Effect of slow freeze versus vitrification on the oocyte: an animal model

Hu, Weihong; Marchesi, Dennis; Qiao, Jie; Feng, Huai L.

doi:10.1016/j.fertnstert.2012.05.037

Cited by 38 publications

(17 citation statements)

References 62 publications

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“…DNA methylation is abnormally and widely reduced after cryopreservation of oocytes according to our previous and other reports (Chen et al, 2016a;Chen et al, 2016b;Hu, Marchesi, Qiao, & Feng, 2012;Liang, Fu, Li, Yuan, & Zhu, 2014). In this study, we found that decreased DNA methylation by vitrification was rescued by Res addition.…”

Section: Discussionsupporting

confidence: 80%

Resveratrol improved the developmental potential of oocytes after vitrification by modifying the epigenetics

Chen

Zhang

Wang

et al. 2019

Molecular Reproduction Devel

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Resveratrol (Res) has been reported to be able to improve oocyte vitrification because of its antioxidative properties. The objective of this study was to further assess the positive effect of Res addition on the developmental potential of vitrified mouse oocytes from the perspective of epigenetic alterations. First, 2 μM Res was chosen as the optimal concentration on the basis of its effects on survival and its antioxidative properties. We found that Res addition significantly promoted fertilization (63.8% vs. 42.9%) and blastocyst formation (68.3% vs. 50.2%) after oocyte vitrification. The quality of the derived blastocysts was also higher after Res treatment. Regarding epigenetic aspects, the expression of the important deacetylase SIRT1 was found to decrease significantly upon vitrification, but it was rescued by Res. The abnormal levels of H3K9 acetylation and DNA methylation in vitrified oocytes were restored by Res addition. Moreover, the expression of several imprinted genes was affected by oocyte vitrification. Among them, abnormal Gtl2 and Peg3 expression levels were restored by Res addition. Therefore, the methylation of their imprinted control regions (ICRs) was examined. Surprisingly, the abnormal patterns of Gtl2 and Peg3 methylation in blastocysts developed from vitrified oocytes were both restored by Res addition. Finally, the full‐term embryonic development showed that the birth rate was improved significantly by Res addition (56.2% vs. 38.1%). Collectively, Res was beneficial for the pre‐ and postimplantation embryonic development. Except for the antioxidative activity, Res also played a role in the correction of some abnormal epigenetic modifications caused by oocyte vitrification.

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Section: Discussionsupporting

confidence: 80%

Resveratrol improved the developmental potential of oocytes after vitrification by modifying the epigenetics

Chen

Zhang

Wang

et al. 2019

Molecular Reproduction Devel

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“…For example, hyperosmotic stress might cause a change in chromatin compactness and DNA accessibility, and cell shrinkage could alter DNA rigidity and bending (Kültz & Chakravarty, ). Some cryoprotectants, such as dimethyl sulfoxide (DMSO) used in this study, have been linked to increased abnormal chromatin despite their function in mitigating freezing damages (Falk et al, ; Hu, Marchesi, Qiao, & Feng, ). During desiccation, DNA is also susceptible to hyperosmotic and dehydration stress (Palazzese, Gosálvez, Anzalone, Loi, & Saragusty, ; Shahba et al, ).…”

Section: Discussionmentioning

confidence: 99%

Desiccation and supra‐zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation

Lee

Comizzoli

2019

Molecular Reproduction Devel

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Understanding cellular and molecular damages in oocytes during exposure to extreme conditions is essential to optimize long‐term fertility preservation approaches. Using the domestic cat (Felis catus) model, we are developing drying techniques for oocytes’ germinal vesicles (GVs) as a more economical alternative to cryopreservation. The objective of the study was to characterize the influence of desiccation on nuclear envelope conformation, chromatin configuration, and the relative fluorescent intensities of histone H3 trimethylation at lysine 4 (H3K4me3) and at lysine 9 (H3K9me3) compared to vitrification. Results showed that higher proportions of dried/rehydrated GVs maintained normal nuclear envelope conformation and chromatin configuration than vitrified/warmed counterparts. Both preservation methods had a similar influence on epigenetic patterns, lowering H3K4me3 intensity to under 40% while maintaining H3K9me3 levels. Further analysis revealed that the decrease of H3K4me3 intensity mainly occurred during microwave dehydration and subsequent rehydration, whereas sample processing (permeabilization and trehalose exposure) or storage did not significantly affect the epigenetic marker. Moreover, rehydration either directly or stepwise with trehalose solutions did not influence the outcome. This is the first report demonstrating that the incidence of GV damages is lower after desiccation/rehydration than vitrification/warming.

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“…Unfortunately, lipid quantification has been limited by its techniques and has yet to be a common assay integrated into the clinical setting. Current approaches for cell lipid quantification include fluorescence labeling [44,58,81], thin layer and gas chromatography [31,37,62,63] and electron microscopy [12,32,79]. However, these destructive techniques are unable to be used towards selections of candidate oocyte for eventual cryopreservation.…”

Section: Oocyte Compositionmentioning

confidence: 99%

“…Though controlled-rate or slow-rate freezing of oocytes can be successful [9][10][11], vitrification has emerged as the apparent preferred approach that yields exceptionally high survival rates and successful live births [19,82]. Numerous review articles have summarized the principles behind both slow-rate cooling and vitrification of cells, including oocytes [9,44,58,74,81,82]. These reviews tend to extensively cover traditional approaches aimed at optimizing these two cryopreservation approaches, which include alterations in solution equilibration temperature, use and optimization of numerous penetrating and non-penetrating CPAs and their combinations, as well as application of various containers that can impact cooling and warming rates.…”

Section: Introductionmentioning

confidence: 99%