Effect of Soaking in Hot Saline Solution and Humid Atmosphere on the Passive Film Behavior of a Ni-Cr-Mo Alloy

Pharkya, Pallavi; Payer, Joe H.

doi:10.2172/893811

“…While these issues fall outside the scope of this study, we observe however that significant progress has been made in developing expression systems that can lead to tunable reaction expression patterns [42,43]. These techniques can expand the applicability of our results as well as of the recently introduced OptReg platform [44], which is a versatile framework that exploits reaction down-and upexpression in the overproduction of targeted compounds.…”

Section: Discussionmentioning

confidence: 88%

Slave nodes and the controllability of metabolic networks

Kim¹,

Motter²

2009

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Recent work on synthetic rescues has shown that the targeted deletion of specific metabolic genes can often be used to rescue otherwise non-viable mutants. This raises a fundamental biophysical question: to what extent can the whole-cell behavior of a large metabolic network be controlled by constraining the flux of one or few reactions in the network? This touches upon the issue of the number of degrees of freedom comprised by one such network. Using the metabolic network of Escherichia coli as a model system, here we address this question theoretically by exploring not only reaction deletions but also a continuous of all possible reaction expression levels. We show that the behavior of the metabolic network can be largely manipulated by the pinned expression of a single reaction. In particular, a relevant fraction of the metabolic reactions exhibit canalizing interactions, in that the specification of one reaction flux determines cellular growth as well as the fluxes of most other reactions in optimal steady states. The activity of individual reaction scan thus be used as surrogates to monitor and possibly control cellular growth and other whole-cell behaviors. In addition to its implications for the study of control processes, our methodology provides a new window to study how the integrated dynamics of the entire metabolic network emerges from the coordinated behavior of its component parts.

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“…The obtained mononitrobenzene (MNB) is catalytically hydrogenated with molecular hydrogen to aniline. Following a potential technology switch, aniline might directly be produced by fermentation, as proposed by Genomatica [108] . However, Covestro pursues the fermentative production of anthranilic acid, which would be converted into aniline by chemical decarboxylation [109] .…”

Section: Discussionmentioning

confidence: 99%

Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals

Hoff¹,

Plassmeier

²

,

Blankschien

³

et al. 2020

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Fermentation as a production method for chemicals is especially attractive, as it is based on cheap renewable raw materials and often exhibits advantages in terms of costs and sustainability. The tremendous development of technology in bioscience has resulted in an exponentially increasing knowledge about biological systems and has become the main driver for innovations in the field of metabolic engineering. Progress in recombinant DNA technology, genomics, and computational methods open new, cheaper, and faster ways to metabolically engineer microorganisms. Existing biosynthetic pathways for natural products, such as vitamins, organic acids, amino acids, or secondary metabolites, can be discovered and optimized efficiently, thereby enabling competitive commercial production processes. Novel biosynthetic routes can now be designed by the rearrangement of nature's unlimited number of enzymes and metabolic pathways in microbial strains. This expands the range of chemicals accessible by biotechnology and has yielded the first commercial products, while new fermentation technologies targeting novel active ingredients, commodity chemicals, and CO2‐fixation methods are on the horizon.

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“…Theo btained mononitrobenzene (MNB) is catalytically hydrogenated with molecular hydrogen to aniline.F ollowing ap otential technology switch, aniline might directly be produced by fermentation, as proposed by Genomatica. [108] However,C ovestro pursues the fermentative production of anthranilic acid, which would be converted into aniline by chemical decarboxylation. [109] Considerations of the product toxicity hampering the host productivity might have determined the most suitable "handover-intermediate" of the intended semisynthesis.T he Covestro technology may use glucose or an alternative 2nd generation carbon source as raw material.…”

Section: New Drop-in Solutions For Commodity Chemicalsmentioning

confidence: 99%

Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals

Hoff¹,

Plassmeier

²

,

Blankschien

³

et al. 2020

View full text Add to dashboard Cite

Fermentation as a production method for chemicals is especially attractive, as it is based on cheap renewable raw materials and often exhibits advantages in terms of costs and sustainability. The tremendous development of technology in bioscience has resulted in an exponentially increasing knowledge about biological systems and has become the main driver for innovations in the field of metabolic engineering. Progress in recombinant DNA technology, genomics, and computational methods open new, cheaper, and faster ways to metabolically engineer microorganisms. Existing biosynthetic pathways for natural products, such as vitamins, organic acids, amino acids, or secondary metabolites, can be discovered and optimized efficiently, thereby enabling competitive commercial production processes. Novel biosynthetic routes can now be designed by the rearrangement of nature's unlimited number of enzymes and metabolic pathways in microbial strains. This expands the range of chemicals accessible by biotechnology and has yielded the first commercial products, while new fermentation technologies targeting novel active ingredients, commodity chemicals, and CO2‐fixation methods are on the horizon.

show abstract

Effect of Soaking in Hot Saline Solution and Humid Atmosphere on the Passive Film Behavior of a Ni-Cr-Mo Alloy

Cited by 3 publications

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Slave nodes and the controllability of metabolic networks

Slave nodes and the controllability of metabolic networks

Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals

Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals

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