Effect of Sodium Alginate Addition to Resveratrol on Acute Gouty Arthritis

Wang, Peng; Ren, Dunlin; Chen, Ying; Jiang, Meiju; Wang, Robin; Wang, Yangang

doi:10.1159/000374064

Cited by 16 publications

(11 citation statements)

References 27 publications

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“…Many previous reports have indicated [36][37][38] that resveratrol can inhibit the inflammationrelated p38MAPK and NF-κB pathways to inhibit the production of inflammatory cytokines, such as COX-2, IL-6, and IL-8, and thereby inhibit the inflammatory cascade response. As a new anti-inflammatory agent, resveratrol has been used extensively in the treatment of many diseases, such as osteoarthritis [39], acute pancreatitis [40], colitis [41], esophagitis [41], acute gouty arthritis [42] and airway inflammation [43]. In the present study, various doses of resveratrol were given to a rat model of postoperative abdominal adhesions, and we found that resveratrol had a significant effect on adhesion prevention.…”

Section: Discussionmentioning

confidence: 53%

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

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Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

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Section: Discussionmentioning

confidence: 53%

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

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“…Notably, a previous study revealed that the addition of sodium alginate to resveratrol, a key effective component of CAGM, significantly enhanced its anti-inflammatory and antioxidant effects. 19 Specifically, the combination of sodium alginate and resveratrol significantly reduced the synovial levels of IL-1b, C-C chemokine receptor type 5 (CCR5), and C-X-C motif chemokine 10 (CXCL10) compared with the effects of colchicine. The combination of sodium alginate and resveratrol was also superior to resveratrol alone in terms of the serum and synovial levels of IL-1b, CCR5, and CXCL10.…”

Section: Discussionmentioning

confidence: 98%

Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice

et al. 2019

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Objective Acute gout is a painful, inflammatory arthritis that features a rapidly escalating inflammatory response resulting from the formation of monosodium urate crystals in the affected joint space. Previously, we found that Chuanhu anti-gout mixture (CAGM) had similar effects as colchicine against gout in the clinic. Subsequently, to improve its effectiveness and efficacy, we modified the original formulation of CAGM. The current study evaluated the effectiveness of the modified formulation in mice. Methods Potassium oxonate (PO) was used to establish a mouse model of hyperuricemia. Plasma levels of uric acid and creatine were determined using the respective test kits. Hepatic xanthine oxidase (XOD) expression was examined by enzyme-linked immunosorbent assay. To explore the underlying mechanism, renal urate transporter 1 (URAT1) mRNA levels were evaluated by quantitative real-time PCR. Allopurinol and benzbromarone were used as reference drugs. Results The original CAGM and its modified high-dose formulation significantly reduced serum uric acid and creatine levels in hyperuricemic mice. In addition, the CAGM-treated groups displayed lower mRNA levels of hepatic XOD and renal URAT1. Conclusions CAGM and its modified formulation significantly ameliorated PO-induced hyperuricemia in mice, which might be partially attributable to reductions of hepatic XOD and renal URAT1 levels.

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“…Resveratrol can also reduce endothelial inflammation by reducing ICAM-1 expression and mediate protection through the miR-221/222/AMPK/p38/NF- κ B pathway [31]. In a previous study, it was shown that resveratrol could effectively treat acute gout arthritis [32]. This agent can significantly reduce joint synovial tissue edema and inflammatory cell infiltration and improve synovial hyperplasia, and its efficacy may be achieved by inhibiting the local aggregation of white blood cells into the inflammatory region and secretion of IL-1 β [33].…”

Section: Discussionmentioning

confidence: 99%

A Randomized Controlled Trial of Chuanhutongfeng Mixture for the Treatment of Chronic Gouty Arthritis by Regulating miRNAs

Wang

Dong

Liu

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Background. We investigated whether Chuanhutongfeng mixture has actions on chronic gouty arthritis (CGA) by regulating miRNAs. Methods. A total of 255 patients with CGA and 30 controls were enrolled. miRNA expression profiles and cluster analysis were preformed; RT-qPCR was used to detect miRNAs associated with CGA. Patients were allocated into Chuanhutongfeng mixture, allopurinol (positive control), and control (etoricoxib) groups. Expression of plasma miRNAs was measured before and after treatments; expression of chemokine 2 (CCL2) and interleukin 8 (CXCL8) was determined by ELISA. Results. 48 miRNAs were differentially expressed and compared to controls. 36 miRNAs expression levels were > 1.5 times and 12 miRNAs < 1.5 times compared to the controls. miR-339-5p, miR-486-5p, and miR-361-5p levels in patients with CGA were lower than in controls (P < 0.05). This trial showed that the Chuanhutongfeng mixture and allopurinol groups had upregulated the expressions of miR-486-5, miR-339-5p, and miR-361-5p and decreased levels of CCL2 and CXCL8 proteins. After 8 weeks of treatment, Chuanhutongfeng mixture decreased serum uric acid levels more than allopurinol (P < 0.05) and reduced levels of CCL2 and CXCL8 protein significantly more than in the allopurinol and control groups. Conclusions. The therapeutic actions of Chuanhutongfeng mixture inhibit the expression of proteins CCL2 and CXCL8 in plasma and upregulated the expressions of three miRNAs (miR-486-5p, miR-339-5p, and miR-361-5p).

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Effect of Sodium Alginate Addition to Resveratrol on Acute Gouty Arthritis

Cited by 16 publications

References 27 publications

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice

A Randomized Controlled Trial of Chuanhutongfeng Mixture for the Treatment of Chronic Gouty Arthritis by Regulating miRNAs

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