Effect of sodium and potassium concentrations and pH on the maintenance of motility of rabbit and rat epididymal spermatozoa

Pholpramool, Chumpol; Chaturapanich, Ganyapong

doi:10.1530/jrf.0.0570245

Cited by 19 publications

(9 citation statements)

References 16 publications

(25 reference statements)

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“…There appears to be an association between high K+ levels in the epididymal plasma and inhibited epididymal sperm movement. Potassium concentrations much higher than those in blood plasma have been reported in the caput and/or cauda fluids of the hamster (39-3 mM; Jessee & Howards, 1976), the elephant (64-8 mM; Jones, 1980), the bull (43-7 mM; Wales, Wallace & White, 1966), the rat (54 mM; Pholpramool & Chaturapanich, 1979) and the rabbit (24-8 mM in the cauda; Jones & Glover, 1973). Boender (1976) showed that boar sperm motility was depressed in media with 45-135 mM-K + and that the motility was restored by washing the same spermatozoa in a medium containing 5 mM-K +.…”

Section: Analysis Of Sperm Movement Characteristics From Video Recordmentioning

confidence: 95%

A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus

Burkman¹,

Overstreet²,

Katz³

1984

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Section: Analysis Of Sperm Movement Characteristics From Video Recordmentioning

confidence: 95%

A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus

Burkman¹,

Overstreet²,

Katz³

1984

Reproduction

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“…However, involvement of other ion translocating systems, such as the ATP-dependent proton pump reportedly present in hamster sperm (22), must be considered. Consideration also must be given to reported differences between mammalian species in the response of sperm motility to variation of media pH and Na' and K+ concentrations (23).…”

mentioning

confidence: 99%

Potassium-dependent increases in cytosolic pH stimulate metabolism and motility of mammalian sperm.

Babcock¹,

Rufo²,

Lardy³

1983

Proc. Natl. Acad. Sci. U.S.A.

133

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Sperm cytosolic pH, determined by the spectral properties of intracellular carboxyfluorescein, is decreased rapidly by the diffusion and subsequent dissociation of the uncharged weak acids pyruvic, lactic, or hydroxybutyric and is increased by diffusion and subsequent intracellular protonation of the weak base NH3. Metabolic and kinetic activity increases dramatically when intracellular pH is elevated above 6.8-6.9 by addition of 50 mM NH4CI to sperm suspended in a 120 mM NaCl medium. Respiratory stimulation is not observed upon comparable additions of 50 mM Li' or K+ or when the pH of the medium is increased from 6.5 to 8.2. However, increases of the external pH to 7.8-8.2 in medium employing 120 mM KCI result in increased metabolic and kinetic activity, comparable to the maximal stimulation induced by the phosphodiesterase inhibitor caffeine. An increase in cytosolic pH from 6.3-6.6 to 6.8 occurs concomitant with the respiratory stimulation induced by KCI in alkaline media. No change in cytosolic pH follows addition of caffeine. Cyclic AMP-dependent protein kinase activity ratios, determined in cellular extracts, are increased by caffeine treatment but are not elevated by 120 mM KCI, by alkaline pH, or by their combination. These observations indicate that cytosolic pH plays a role in the regulation of motility and metabolism ofmammalian sperm that is not mediated by cyclic AMP but that may be under control of a plasma membrane voltage-dependent proton channel. However, H+ fluxes across vesicles prepared from sperm membranes are unaffected by variation in the magnitude of the transvesicular K+ concentration gradient.Processes mediated by the intracellular concentrations ofcyclic AMP (1,2) and Ca2+ (3,4) play important roles in regulating function in both invertebrate and mammalian spermatozoa (5, 6). Initial observations of the consequences of treating intact sperm with specific ionophores (7) and of the pH dependence of the activity of isolated sperm components in vitro (8) indicated that intracellular pH also may control exocytotic or contractile events in these cells. In addition a Na+/H+ exchange mechanism has been implicated in the initiation of rat sperm motility (9) and in the stimulation of sperm motility and respiration that is induced by the "speract" peptide released from sea urchin eggs (10).These contentions were supported by estimations of intracellular pH before (10-12) and after (11) the exocytotic acrosome reaction of sea urchin (11) or hamster (12) sperm and by apparent changes in internal pH during Na+-dependent initiation of sea urchin (13) and rat (9) sperm motility. However, the observed net proton release from sperm suspensions (10) or the changes in the pH of cell homogenates (9) or in the distribution of fluorescent amines (11-13) are difficult to relate to true quantitative alterations in intracellular pH.Recently the spectral properties ofcarboxyfluorescein, generated intracellularly from its permeant diacetate derivative, were used to determine cytosolic pH of ascites tumor...

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“…Pyknotic or shed epithelial cells of the epididymis and abnormal values of pH, osmotic pressure, and Na + ·K + concentrations in seminal plasma have been reported to be associated with the production of sperm with abnormal motility and morphology [7,13,14,30,32]. There is a report [11] on dogs stating that the epididymis and the proximal portion of the vas deferens are not only passage way for spermatozoa but accessory reproductive organs of spermatozoal maturation and storage.…”

Section: Discussionmentioning

confidence: 99%

Changes in Semen Quality and In Vitro Sperm Capacitation during Various Frequencies of Semen Collection in Dogs with Both Asthenozoospermia and Teratozoospermia.

1998

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ABSTRACT. Eight male dogs with both asthenozoospermia and teratozoospermia were used in this study. In experiment 1, semen was collected 10 times at intervals of 48 hr, 24 hr and 12 hr in 4 of the 8 dogs, and the semen quality was evaluated. In experiment 2, semen was collected 5 times at 24-hr intervals in the other 4 dogs. The spermatozoa collected on day 1 and day 5 were incubated for 4-6 hr in Canine Capacitation Medium, and the percentages of hyperactivated sperm (%HA), acrosome-reacted sperm (%AR), and the zona pellucida-binding sperm count (ZP sperm count) were assessed. The results of experiment 1 showed that the percentage of motile sperm increased and the percentage of abnormal sperm decreased markedly as the intervals between semen collections became shorter. When semen was collected at 12-hr intervals, the percentage of motile sperm increased from about 65%, the value before frequent collection was started, to about 80%, and the percentage of sperm with abnormal tails decreased from 30% to15%. In experiment 2, the percentages of HA and AR, and the ZP sperm count in specimens collected on day 5 were higher than those in specimens collected on day 1, and the differences in % HA and in ZP sperm count were significant (P<0.05). The results demonstrated that sperm motility, abnormality, and potential fertility in dogs with asthenozoospermia and teratozoospermia can be temporarily improved by frequent semen collection. -KEY WORDS: asthenozoospermia, canine, frequent semen collection, teratozoospermia.J. Vet. Med. Sci. 60 (5): [607][608][609][610][611][612][613][614] 1998 years, were used. The body weight of the Beagle dogs and the mongrel dogs ranged from 12 kg to 15 kg, and the Shetland sheep dog weighed 20 kg. All of the dogs were diagnosed as having both asthenozoospermia and teratozoospermia, based on a comparison of their semen quality (Table 1) and with other reports on normal semen quality in dogs [17,20,38]. The dogs were housed in pens with runs under natural lighting, and water was available ad libitum. Semen evaluation: The male dogs were trained so that their semen could be collected by digital manipulation without an estrous bitch. Semen samples were examined for total semen volume, total number of sperm, and percentages of actively motile sperm, viable sperm, and morphologically abnormal and immature sperm using methods previously described [23]. Briefly, the concentration of sperm in the semen was determined by hematocytometer counts. The percentages of motile sperm and viable sperm were estimated by counting actively motile sperm on a warmed slide glass and by the eosin-nigrosin staining method, respectively. Sperm morphology was examined by the aniline blue and eosin staining method [5]. Spermatozoa with a cytoplasmic droplet attached to the midpiece were counted as immature sperm [39]. It has been known that the ejaculate in dogs can be divided into 3 fractions [15]. In the present study, the pH and osmolarity (Vapor Pressure Osmometer; Model 5100, Wescor, Inc., U.S.A.) of the second s...

show abstract

Effect of sodium and potassium concentrations and pH on the maintenance of motility of rabbit and rat epididymal spermatozoa

Cited by 19 publications

References 16 publications

A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus

A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus

Potassium-dependent increases in cytosolic pH stimulate metabolism and motility of mammalian sperm.

Changes in Semen Quality and In Vitro Sperm Capacitation during Various Frequencies of Semen Collection in Dogs with Both Asthenozoospermia and Teratozoospermia.

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