Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Abe, Miyako; Küfe, Donald

doi:10.1007/bf01806038

Cited by 29 publications

(22 citation statements)

References 11 publications

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“…These alterations include induction of differen tiated characteristics, such as dome forma tion and lipid accumulation [6][7][8]. Sodium butyrate was also shown to induce specific proteins of breast carcinoma [7], The present results show that sodium butyrate and DMSO exert similar anti-proliferative ef fects on MCF-7 cells (table I).…”

Section: Discussionsupporting

confidence: 61%

“…Dimethylsulfoxide (DMSO) and sodium butyrate are potent chemical inducers of dif ferentiation of hematopoietic cell lines [1][2][3], Sodium butyrate and DMSO have also been shown to reduce the neoplastic poten tial of many solid tumor cell lines [4,5], including breast cancer cell lines [6][7][8]. The anti-proliferative effects of differentiating agents were accompanied by phenotypic al terations, such as morphological and ultrastructural changes [9][10][11][12], alterations in en zyme activities [4,11 ], receptor content and cell products [4][5][6][8][9][10][11][12][13][14][15], In this study we examined the effects of DMSO and sodium butyrate on MCF-7 breast cancer cell proliferation and on the activities of y-glutamyl transpeptidase and alkaline phosphatase.…”

Section: Introductionmentioning

confidence: 99%

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Differential Effects of Sodium Butyrate and Dimethylsulfoxide on Gamma-Glutamyl Transpeptidase and Alkaline Phosphatase Activities in MCF-7 Breast Cancer Cells

Wasserman

Nordenberg²,

Beery³

et al. 1987

Pathobiology

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Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semi-solid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and γ-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in γ-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Differential Effects of Sodium Butyrate and Dimethylsulfoxide on Gamma-Glutamyl Transpeptidase and Alkaline Phosphatase Activities in MCF-7 Breast Cancer Cells

Wasserman

Nordenberg²,

Beery³

et al. 1987

Pathobiology

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“…DF3 antigen expression also correlates with the degree of breast tumor differentiation and estrogen receptor status (2). These findings and the detection of DF3 antigen in human milk (3) have suggested that mAb DF3 reacts with a differentiation antigen expressed in breast carcinoma cells.…”

Section: Introductionmentioning

confidence: 72%

Isolation and sequencing of a cDNA coding for the human DF3 breast carcinoma-associated antigen.

Siddiqui

Abe

Hayes

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

271

154

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The murine monoclonal antibody (mAb) DF3 reacts with a high molecular weight glycoprotein detectable in human breast carcinomas. DF3 antigen expression correlates with human breast tumor differentiation, and the detection of a cross-reactive species in human milk has suggested that this antigen might be useful as a marker of differentiated mammary epithelium. To further characterize DF3 antigen expression, we have isolated a cDNA clone from a Agtll library by screening with mAb DF3. The results demonstrate that this 309-base-pair cDNA, designated pDF9.3, codes for the DF3 epitope. Southern blot analyses of EcoRI-digested DNAs from six human tumor cell lines with 32P-labeled pDF9.3 have revealed a restriction fragment length polymorphism. Variations in size of the alleles detected by pDF9.3 were also identified in Pst I, but not in HindIII, DNA digests. Furthermore, hybridization of 32P-labeled pDF9.3 with total cellular RNA from each of these cell lines demonstrated either one or two transcripts that varied from 4.1 to 7.1 kilobases in size. The presence of differently sized transcripts detected by pDF9.3 was also found to correspond with the polymorphic expression of DF3 glycoproteins. Nucleotide sequence analysis of pDF9.3 has revealed a highly conserved (G+C)-rich 60-base-pair tandem repeat. These rmdings suggest that the variation in size of alleles coding for the polymorphic DF3 glycoprotein may represent different numbers of repeats.A human breast carcinoma-associated antigen has been identified by using a murine monoclonal antibody (mAb), designated DF3. mAb DF3 was prepared against a membrane-enriched fraction of a human breast carcinoma metastatic to liver (1). DF3 antigen is expressed on the apical borders of secretory mammary epithelial cells and in the cytosol of less differentiated malignant cells (1). DF3 antigen expression also correlates with the degree of breast tumor differentiation and estrogen receptor status (2). These findings and the detection of DF3 antigen in human milk (3) The present studies were performed to further examine genetic mechanisms responsible for the heterogeneity of DF3 antigen expression. We describe the isolation of a partial cDNA clone,t designated pDF9.3, coding for the DF3 antigen. This cDNA clone encompasses 309 nucleotides of DF3 mRNA and has tandemly repeated sequences. By using this clone, we also demonstrate that the heterogeneity of DF3 antigen is related to size variations in DF3 alleles and DF3 transcripts. MATERIALS AND METHODSLibrary Screening. An oligo(dT)-primed cDNA library prepared from human MCF-7 breast carcinoma cells in Agtll was kindly provided by P. Chambon (Institut de Chimie Biologique, Strasbourg, France) (8). Immunologic screening of the Agtll library was performed as described (9) by using affinity-purified mAb DF3 (0.25 ,ug/ml) and antimouse IgG conjugated with alkaline phosphatase (Promega Biotec, Madison, WI). Positive plaques were isolated and the phage was further purified to homogeneity by repeated antibody screening. DNA was i...

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“…DF3 antigen expression correlates with the degree of breast tumor differentiation (4). Moreover, the detection of this antigen in human milk (5) has suggested that its expression represents a differentiated function of normal mammary epithelium. Other work has demonstrated that this family of glycoproteins regulates immune recognition and cellular adhesion (6,7).…”

mentioning

confidence: 99%

Characterization of cis-acting elements regulating transcription of the human DF3 breast carcinoma-associated antigen (MUC1) gene.

Abe

Küfe

1993

Proc. Natl. Acad. Sci. U.S.A.

100

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The present studies have examined the sequences responsible for regulating transcription of the human DF3 breast carcinoma-associted antigen (MUCI) gene. A region 1656 base pairs upstream to the DF3 transcription initiation site was fused to the chloramphenical acetyltransferase gene. Transient expression assays using a series of deleted constructs demonstrated that the region frown position -618 contains the regulatory sequences necessary for DF3 transcription in human MCF-7 breast cancer cells. Further analysis with internal deletion vectors and heterologous promoter constructs indicated the involvement of cis-acting elements in the fragment extending from positions -598 to -485. By gel retardation and DNA footprinting, we have identified a protein in MCF-7 cells that recognizes sequences between positions -505 and -485. The results of Southwestern studies demonstrate that this protein has an apparent molecular mass of 45 kDa. Taken together, these results suggest that DF3 gene transcription is regulated by a previously undescribed transacting factor.

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Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Cited by 29 publications

References 11 publications

Differential Effects of Sodium Butyrate and Dimethylsulfoxide on Gamma-Glutamyl Transpeptidase and Alkaline Phosphatase Activities in MCF-7 Breast Cancer Cells

Differential Effects of Sodium Butyrate and Dimethylsulfoxide on Gamma-Glutamyl Transpeptidase and Alkaline Phosphatase Activities in MCF-7 Breast Cancer Cells

Isolation and sequencing of a cDNA coding for the human DF3 breast carcinoma-associated antigen.

Characterization of cis-acting elements regulating transcription of the human DF3 breast carcinoma-associated antigen (MUC1) gene.

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