The process of infection of potato varieties with different resistance to wart by zoospores of the causative agent of the disease was studied. Experients carried out in laboratory conditions in artificial infectious background for the damage of the Poliska rozheva potato variety (susceptible to all pathotypes of potato wart) and Slovyanka (resistant to the common pathotype and susceptible to all pathotypes of the causative agent of potato wart existing in Ukraine by summer zoospores of the pathotypes of the causative agent of the disease . Determination of the content of total proteins was carried out according to the Marion Bradford’s method. Determination of peroxidase et polyphenoloxidase activity was determined according to the method of Kabar A. M., ZaikoG. A., Liholat T. Yu. et Tsivinska M. V., Antonyuk V. O., Stoika R.S. As a result of studies conducted to determine the pathogenesis of potato wart in the plant-host-pathogen system, in Poliska rozheva potato variety (susceptible to all wart pathotypes), soruses with zoospores of the wart pathogen observed on the affected sprouts, in potato variety Slovyanka, soruses with zoospores observed for lesions of aggressive pathotypes. When affected by the common pathotype of the causative agent of wart, soruses of the pathogen absent. Cells of the epidermis are necrotic. In the case of damage to susceptible potato varieties by pathotypes of the causative agent of wart, the content of total proteins increased. It varied between 0.221-0.262 mg/ml for the Poliska rozheva variety and 0.225-0.260 for the Slavyanka variety. The activity of peroxidase redox enzymes during affection by pathotypes was 0.046-0.053 μmol per minute, polyphenoloxidase – 0.050-0.057.
Thus, in the pathogenesis of the plant-host-pathogen for damage to potatoes by pathotypes of the potato wart pathogen Synchytrium endobioticum (Schilbersky) Percival, deviations in the development of structural proteins of the epidermis and different activity of oxidative enzymes were found. Based on these biochemical indicators, biochemical methods of identification of potato cancer pathotypes that exist in Ukraine have been developed and patented.
Key words: potato, wart, pathogenesis, protein composition, redox enzymes, activity, identification, pathotypes
