Effect of Sodium Fluoride on LS Cells

Hongslo, Jan K.; Holland, Roy I.; Jonsen, Jon

doi:10.1177/00220345740530023801

Cited by 36 publications

(21 citation statements)

References 9 publications

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“…acting upon any component in serum, in agreement with previous reports (Hongslo et at. 1974;Ekstrand et al 1977).…”

Section: Resultssupporting

confidence: 93%

“…Fluoride inhibition of protein and DNA synthesis correlates well with its growth inhibiting effect. As no other parameter, such as metabolism and ATP content (Holland & Hongslo 1978& 1979a, uptake and pools of amino acids (Holland & Hongslo 1979b), Na', K+ levels (Quissell & Suttie 1973), is significantly affected at such low fluoride concentrations as presently found to inhibit protein and DNA synthesis, it seems probable that these are the main targets for the cytotoxic action of fluoride. This is consistent with results recently published by Mankowitz et al (1978).…”

Section: Resultsmentioning

confidence: 99%

“…LS cells (mouse fibroblasts in suspension culture) were grown in Eagle's minimum essential medium for suspension culture supplemented with 10% heat inactivated (56", 30 min.) calf serum and 0.5% peptone (Hongslo et al 1974). Incorporarion of 'H-ihymidine and 14C-leucine.…”

Section: Methodsmentioning

confidence: 99%

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Fluoride Inhibition of Protein and DNA Synthesis in Cells in Vitro

Holland

1979

Acta Pharmacologica et Toxicologica

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Fluoride concentrations at and above 0.9 mM caused a progressive, concentration‐related inhibition in the incorporation of both 14C‐leucine and 3H‐thymidine in LS cells incubated in medium with serum. The incorporation of leucine was more affected than that of thymidine, Lowering the pH enhanced the effect of fluoride on both. Removing serum from the incubation medium changed the effect of fluoride, particularly at low pH (7.0). Incorporation of leucine was then stimulated by low fluoride concentrations (0.5 and 0.9 mM), and the effect on thymidine incorporation was eradicated up to 1.3 mM‐NaF. No differences were found in the pool and the specific activity of 14C‐leucine in the fluoride exposed cells compared to control cells without fluoride (incubated at pH 7.4 in medium without serum). The cellular pool of 3H‐thymidine decreased markedly during the incubation period, somewhat less in the fluoride exposed cells than in the control.

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“…acting upon any component in serum, in agreement with previous reports (Hongslo et at. 1974;Ekstrand et al 1977).…”

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Fluoride Inhibition of Protein and DNA Synthesis in Cells in Vitro

Holland

1979

Acta Pharmacologica et Toxicologica

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“…Early studies in 1970s reported that by stepwise increase in fluoride concentration, cells adapted to grow at fluoride concentrations that would kill them . Several types of cells (e.g., L cells, LS cells, Hela cells, and CHO cells) that retained their vitality in lethal concentrations of fluoride were developed by slowly increasing the concentration of fluoride . However, due to limitations in research methodology, no further research was performed at molecular level to investigate mechanisms associated with the observation of fluoride resistance of cells.…”

Section: Discussionmentioning

confidence: 99%

Fluoride resistance capacity in mammalian cells involves complex global gene expression changes

et al. 2017

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Fluorine is a bone‐seeking element ubiquitously present in the environment and widely used in many oral hygiene products. In humans, excessive intake of fluoride may cause dental and skeletal fluorosis. However, endemic fluorosis does not appear to develop in a proportion of individuals exposed to the same levels of fluoride. The mechanisms by which mammalian cells resist fluoride are still unclear. In this study, we developed strains of mouse L‐929 cells resistant to different levels of fluoride. High‐throughput RNA‐sequencing analyses of the fluoride‐resistant L‐929 cells indicated that massive changes in global gene expression occurred, compared with the wild‐type L‐929 cells. The main biological processes and functions changed were associated with the extracellular region and matrix, response to stress, receptor binding, and signal transduction. This indicated that high doses of fluoride not only exerted stress on L‐929 cells but also induced functional pathways that helped them adapt to the presence of fluoride or to expel it. These data should prove useful in identifying cellular processes or transporters/channels that play central roles in adaptation to or expulsion of fluoride in humans.

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“…The introduction of fluoride application has been a controversial and political process which has centered around questions of biological risks involved when the fluoride is used in the public water supply (Richmond 1985). In this context, fluoride cytotoxicity was mostly of concern and was examined by numerous earlier workers using established aneuploid cell lines (Carlson & Suttie 1967;Hongslo et al 1974;Oguro et al 1982). However, its effect on growth of human diploid cells, as normal cell model, is less known (Tsutsui et al 1984).…”

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confidence: 99%

Effect of Sodium Fluoride on Growth of Human Diploid Cells in Culture

Oguro

Cervenka

Horii

1990

Pharmacology & Toxicology

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Cytotoxicity of sodium fluoride (NaF) on human diploid cells in exponential growth was investigated using a) Flow 1000 cells, passage No. 13, obtained from skin and muscle tissues of male black foetus, b) IMR-90 cells, passage No. 22, derived from lung tissue of female Caucasian foetus and c) primary fibroblast-like cell cultures from 5 Japanese whole foetuses. Diploid cells did not survive at 20 p.p.m. of ionic fluoride (F-) concentration. However, the cells were capable of proliferation with no significant impairment of growth up to 0.2 p.p.m. F-, a level which is much higher than the plasma concentration in human subjects from areas with highly fluoridated water. The growth of the cells was markedly affected by F- concentrations greater than 2 p.p.m.

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Effect of Sodium Fluoride on LS Cells

Cited by 36 publications

References 9 publications

Fluoride Inhibition of Protein and DNA Synthesis in Cells in Vitro

Fluoride Inhibition of Protein and DNA Synthesis in Cells in Vitro

Fluoride resistance capacity in mammalian cells involves complex global gene expression changes

Effect of Sodium Fluoride on Growth of Human Diploid Cells in Culture

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