Effect of sodium nitroprusside on micropropagation and biochemicalparameters of CAB-6P and Gisela 6 cherry rootstocks

Abstract

The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 µM 6-benzylaminopurine (BA) and SNP. Gisela 6 microcuttings treated with BA + 30 µM SNP gave the highest average shoot number (4.15) and multiplication percentage (100%). In CAB-6P, α-naphthaleneacetic acid (NAA) + 30 µM SNP led to the maximum root number (13.79) and fresh weight (0.336 g); average root length (82 mm) and rooting percentage (95.45%) were greatest after adding 40 and 10 µM SNP + NAA, respectively. In Gisela 6, NAA + 40 µM SNP resulted in the highest root number (9.75) and fresh weight (0.238 g), while NAA + 30 µM SNP enabled maximum root length (30.77 mm). Rooting percentage was greatest (84.62%) when Gisela 6 microcuttings were treated only with NAA. Application of SNP alone in CAB-6P reduced leaf chlorophyll, carbohydrate, and prοline levels. In contrast, 30-50 µM SNP increased leaf chlorophyll levels of Gisela 6. SNP augmented leaf carbohydrate and proline content, but it diminished root carbohydrate content. In BA + SNP or NAA + SNP treatments, changes in biochemical constituents were dependent on SNP concentrations and genotypes.

Effects of sodium nitroprusside and growth regulators on callus, multiple shoot induction and tissue browning in commercially important Valeriana jatamansi Jones

“…The treatments with three other concentrations also failed to produced proficient shoot induction. Similar to our results, previously a 100% callus induction percentage in cherry rootstocks (Sarropoulou et al 2015) and 87% callus induction in D.…”

“…The positive effects of exogenous supplementation of NO and/or SNP on in vitro propagation has been demonstrated in various plant species including Arabidopsis, tobacco, and parsley (Tun et al 2001), cucumber (Pagnussat et al 2002), mung bean (Huang andShe 2003), tomato (Correa-Aragunde et al 2004), Chinese crab apple (Han et al 2009), Dioscorea opposita (Xu et al (2009), cherry rootstocks (Sarropoulou et al 2015) and Canscora diffusa (Subiramani et al 2019). All these reports indicate that NO and/or SNP plays a role in cell division, as well as in shoot regeneration and multiplication.…”

Indirect regeneration in Ficus lyrata Warb. and metabolite profiles influenced by nitric oxide and Plant growth regulators

“…Our findings here on F. lyrata , together with findings of several other studies in various plant species have proved that synergistic interaction of NO × CKs × auxin could strongly promote callogenesis, morphogenic callus induction, and shoot regeneration. This was supported by the reports in Malus hupehensis [ 26 ], Albizzia lebbeck [ 27 ], Vanilla planifolia [ 28 ], Cherry rootstocks [ 29 , 30 ], Malus niedzwetzkyana [ 31 ], etc. The synergistic cross-talk of NO × CK × auxin dramatically stimulated cell dedifferentiation and cell proliferation in Arabidopsis [ 32 ] by augmenting the auxin and CK signaling pathways, and resulted in new meristematic centers.…”

Sodium Nitroprusside Stimulates Micropropagation and TDZ Induces Adventitious Shoots Regeneration in Red Flesh Apple Malus niedzwetzkyana Koehne Dieck ex

“…Beligni and Lamattina (2000) reported that NO affects plant developmental events in which gibberellins are essential in order for them to operate. Although many reports imply that SNP has positive effects on shoot proliferation of in vitro plantlets, Sarropoulou et al (2015) reported that combination of SNP and BAP reduced in vitro shoot proliferation of cherry rootstock. Verma et al (2014) also reported that, when SNP was used at a concentration of 500 µM, proliferation of plantlets declined and leaves turned brown and necrotized in two peanut cultivars.…”