“…Five biofilm annular reactors (Model 1320 LS; Biosurface Technologies Corp., Bozeman, Montana) were operated for 13 months, from September 30, 2019 to October 21, 2020. Annular reactors have been commonly used as bench-scale models to mimic water distribution systems and to simulate biofilm growth 4 , 12 , 83 , 84 . The annular reactors comprised rotating inner cylinders that subjected biofilm growing on removable, flush-mounted coupons to shear forces typical of water systems, independent of flow-controlled residence time.…”