Effect of sodium tanshinone IIA sulfonate in the rabbit myocardium and on human cardiomyocytes and vascular endothelial cells

Wu, Tai-Wing; Zeng, Ling-Hua; Fung, Kwok‐Pui; Wu, Jun; Pang, Henrianna; Grey, Arthur A.; Weisel, Richard D.; Wang, Jian Yao

doi:10.1016/0006-2952(93)90624-6

Cited by 109 publications

(68 citation statements)

References 22 publications

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“…Oxygen radicals are markedly produced after a hypoxic-ischemic insult. Several lines of evidence have shown that tanshinone IIA can reverse the abnormalities induced by free radical injury as previously described by our laboratory and other researchers (26,32). It could also protect the mitochondrial membrane from ischemia-reperfusion injuries and lipid peroxidation (33).…”

supporting

confidence: 72%

Partial Neuroprotective Effect of Pretreatment with Tanshinone IIA on Neonatal Hypoxia-Ischemia Brain Damage

et al. 2005

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Tanshinone IIA is a compound purified from the Chinese herb Danshen (Radix Salviae Miltiorrhiza Bge). The neuroprotective effect of tanshinone IIA was investigated in a neonatal rat model of hypoxia-ischemia brain damage. Hypoxia-ischemia encephalopathy was induced in rats at day 7 of postnatal age by ligation of the right common carotid artery, followed by 2 h of hypoxia. Tanshinone IIA (10 mg/kg, i.p.) was injected daily from day 2 before surgery for 9 or 16 d. Our results demonstrated significant and sustained brain damage in the hypoxia-ischemia-and vehicle-treated groups at 1 and 3 wk after surgery. Treatment with tanshinone IIA significantly reduced the severity of brain injury, as indicated by the increase in ipsilateral brain weight and neuron density, compared with those of sham-operated animals. The recovery of sensorimotor function and histology was observed in animals that received tanshinone IIA. The plasma of tanshinone IIA-treated rats exhibited higher antioxidant activities, as reflected by the oxygen radical absorbance capacity assay, compared with the vehicle-treated rats. In the neural progenitor cell line C17.2 that was subjected to 2,2'-azobis (2-amidino propane hydrochloride)-induced oxidative stress, tanshinone IIA increased cell viability and protected against mitochondrial damage (JC-1 assay). Our results suggest that tanshinone IIA has antioxidative activities and that treatment that is started before a hypoxic-ischemic insult is partially neuroprotective. Further studies are required to elucidate whether rescue treatment with tanshinone IIA is effective and to determine whether its protective effect is also associated with secondary cooling of the brain. Abbreviations AAPH, 2,2'-azobis (2-amidino propane hydrochloride) AchE, acetylcholinesterase HIE, hypoxia-ischemia encephalopathy MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide NSE, neuron-specific enolase ORAC, oxygen radical absorbance capacity Hypoxia-ischemia encephalopathy (HIE) in the newborn infant is a major cause of acute mortality and chronic neurologic disability in survivors. During hypoxic-ischemic brain injury, mitochondrial functions are compromised and the lack of electron acceptors leads to free radical leakage from the mitochondria into the cytoplasm, causing lipid peroxidation of polyunsaturated fatty acid-rich neuronal membranes (1,2).Danshen is derived from the dried root or rhizome of Salviae Miltiorrhizae Bge. In recent decades, Danshen has been commonly used in China for the treatment of angina pectoris and cerebrovascular disorders such as ischemic stroke (3). Tanshinone IIA, a derivative of phenanthrenequinone, is one of the key components of Danshen (Fig. 1). It has antioxidant properties and has been demonstrated to protect against lipid peroxidation in in vitro and in vivo studies (4,5). Tanshinone IIA can prevent the neuroblastoma cell line PC12 from apoptosis induced by serum-free culture conditions (6). It has the potential to penetrate the blood-brain barrier and acts as an inhibitor...

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confidence: 72%

Partial Neuroprotective Effect of Pretreatment with Tanshinone IIA on Neonatal Hypoxia-Ischemia Brain Damage

et al. 2005

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“…Danshen refers to a medicinal herb (termed shen) containing substance of premier medicinal value (termed Dan, cinnabar), and has been widely used in China for patients with cardiovascular disorders (23). Its beneficial effects are attributable to several red pigments including tanshinones I, II, IV, and cryptotanshinone (24,25), which exhibit various antiinflammatory properties (26 -28). In this study, we evaluated their capacity in inhibiting endotoxin-induced HMGB1 release, and explored their therapeutic potential in animal models of lethal endotoxemia and sepsis.…”

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confidence: 99%

A Cardiovascular Drug Rescues Mice from Lethal Sepsis by Selectively Attenuating a Late-Acting Proinflammatory Mediator, High Mobility Group Box 1

Ashok

et al. 2007

The Journal of Immunology

129

171

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The pathogenesis of sepsis is mediated in part by bacterial endotoxin, which stimulates macrophages/monocytes to sequentially release early (e.g., TNF, IL-1, and IFN-γ) and late (e.g., high mobility group box 1 (HMGB1) protein) proinflammatory cytokines. The recent discovery of HMGB1 as a late mediator of lethal sepsis has prompted investigation for development of new experimental therapeutics. We found that many steroidal drugs (such as dexamethasone and cortisone) and nonsteroidal anti-inflammatory drugs (such as aspirin, ibuprofen, and indomethacin) failed to influence endotoxin-induced HMGB1 release even at superpharmacological concentrations (up to 10–25 μM). However, several steroid-like pigments (tanshinone I, tanshinone IIA, and cryptotanshinone) of a popular Chinese herb, Danshen (Salvia miltiorrhiza), dose dependently attenuated endotoxin-induced HMGB1 release in macrophage/monocyte cultures. A water-soluble tanshinone IIA sodium sulfonate derivative (TSNIIA-SS), which has been widely used as a Chinese medicine for patients with cardiovascular disorders, selectively abrogated endotoxin-induced HMGB1 cytoplasmic translocation and release in a glucocorticoid receptor-independent manner. Administration of TSNIIA-SS significantly protected mice against lethal endotoxemia and rescued mice from lethal sepsis even when the first dose was given 24 h after the onset of sepsis. The therapeutic effects were partly attributable to attenuation of systemic accumulation of HMGB1 (but not TNF and NO) and improvement of cardiovascular physiologic parameters (e.g., decrease in total peripheral vascular resistance and increase in cardiac stroke volume) in septic animals. Taken together, these data re-enforce the pathogenic role of HMGB1 in lethal sepsis, and support a therapeutic potential for TSNIIA-SS in the treatment of human sepsis.

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“…22) PA also participated in the actions of Salvia Root on myocytes. 23) However, the knowledge of the cardiovascular activities of Salvia Root had begun with tanshinones and focused on for a long time, [24][25][26] which resulted in that TaII A had been used as the chemical marker for standardization of the crude drug and its phytomedicines.…”

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Quantitative Analysis of the Marker Compounds in Salvia miltiorrihiza Root and Its Phytomedicinal Preparations

Yuan

Pan

et al. 2005

Chem. Pharm. Bull.

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A HPLC method is described in order to separate and identify 4 phenolic compounds including lithospermic acid B, 3,4-dihydroxyphenyllactic acid (danshensu), rosmarinic acid, and protocatechuic aldehyde as well as 3 lipophilic ones (tanshinone I, tanshinone II A and cryptotanshinone) from the roots of Salvia miltiorrihiza or the herbal product containing S. miltiorrihiza available in Chinese or Japanese market. The influence of extractive conditions, such as method, solvents and time, on the 4 phenolic marker components in S. miltiorrihiza is well discussed. In order to investigate the stability of lithospermic acid B, the preparation samples of an injection and a granule were analyzed after being stored at 4°C, 20°C and 40°C for up to six months.

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Effect of sodium tanshinone IIA sulfonate in the rabbit myocardium and on human cardiomyocytes and vascular endothelial cells

Cited by 109 publications

References 22 publications

Partial Neuroprotective Effect of Pretreatment with Tanshinone IIA on Neonatal Hypoxia-Ischemia Brain Damage

Partial Neuroprotective Effect of Pretreatment with Tanshinone IIA on Neonatal Hypoxia-Ischemia Brain Damage

A Cardiovascular Drug Rescues Mice from Lethal Sepsis by Selectively Attenuating a Late-Acting Proinflammatory Mediator, High Mobility Group Box 1

Quantitative Analysis of the Marker Compounds in Salvia miltiorrihiza Root and Its Phytomedicinal Preparations

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