Effect of sole or combined administration of nitrate and 3-nitro-1-propionic acid on fermentation and Salmonella survivability in alfalfa-fed rumen cultures in vitro

Correa, Alejandro Castañeda; Trachsel, Julian; Allen, Heather K.; Corral-Luna, Agustin; Bañuelos, Héctor Gutiérrez; Ochoa-Garcia, Pedro Antonia; Ruiz-Barrera, Oscar; Hume, Michael E.; Callaway, Todd R.; Harvey, Roger B.; Beier, Ross C.; Anderson, Robin C.; Nisbet, David J.

doi:10.1016/j.biortech.2017.01.012

Cited by 7 publications

(8 citation statements)

References 45 publications

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“…This discrepancy may be ascribed to the different doses of 3NPA used among these studies as the effect of 3NPA on total gas production was dose-dependent [ 3 ]. Moreover, in contrast to the prior studies reported that the CH 4 production reduced significantly with the supplementation of 3NPA [ 3 , 16 , 27 ], CH 4 production showed an increased trend from 0 to 8 mM 3NPA for 12, 24, and 48 h incubation in this study. This finding suggests that the effect of 3NPA on inhibition of ruminal methane production is divergent between grazing yak and other ruminant species regarding the animal species affects ruminal fermentation characteristics [ 28 ], and further studies based on metagenome and metabolome could be conducted to dig out the potential mechanism behind this.…”

Section: Discussioncontrasting

confidence: 99%

“…By comparison, some leguminous plants (i.e., Astragalus ) and fungi ( Aspergillus , Penicillium , and Arthrinium ) [ 14 ] contain 3NPA that can be converted to products (b-alanine and 3-amino-1-propanol, respectively) by rumen microbiota and regarded as carbon, nitrogen, and energy by the host [ 15 ]. In addition, some forage species containing 3NPA could be used to feed ruminants safely after hay; although, it is toxic to the livestock [ 16 ]. Additionally, 3NPA is less toxic than NPOH, and the ruminants grazing on the Qinghai–Tibetan plateau could consume a moderate number of poisonous plants without obvious poisonous symptoms [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Guo,

Wang,

Zhang

et al. 2024

Animals

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3-nitropropionic acid (3NPA) has been proposed as an useful modifier to mitigate ruminal enteric methane emissions. However, few studies investigated the effects of 3NPA on ruminal fermentation characteristics of grazing ruminants in vitro. Rumen fluid from grazing yak and cattle were collected and incubated with additions of 0, 8, and 16 mM 3NPA. The total gas production, CH4 production, and dry matter digestibility significantly decreased with increasing 3NPA doses in both ruminant species (p < 0.05) and methane production decreased to almost 100% in cattle at 8 mM NPA but not yak, while H2 accumulation showed an opposite trend. The total fatty acid (TVFA) production, acetate concentration, and propionate concentration in cattle decreased as 3NPA doses increased at 12 and 24 h incubation. For yak, the H2 accumulation reached its apex at 8 mM NPA (p < 0.05). The TVFA in yak decreased significantly with increasing 3NPA doses at 12 and 72 h incubation. Moreover, the acetate concentration and propionate concentration in yak decreased as 3NPA doses increased at 12 and 24 h incubation. Overall, these findings demonstrated that 3NPA could be used as a strategy to mitigate methane emissions; although, it negatively affected the dry matter degradability in vitro.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Guo,

Wang,

Zhang

et al. 2024

Animals

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“…While ethyl nitroacetate exhibited quite modest antimicrobial activity against E. coli O157:H7 and S. Typhimurium, it was very potent at modulating methane production. Likewise, reductions in methane production observed in all the nitrocompound-treated incubations in the present screening study are consistent with observations from earlier studies conducted either with 100% carbon dioxide, as was performed in the present study [ 13 , 38 , 39 ], or with a 50:50 hydrogen:carbon dioxide as the initial gas phase [ 13 , 40 , 41 ]. The studies initiated with the 50:50 hydrogen:carbon dioxide gas phase were designed to ensure the provision of a non-limiting amount of reducing substrate.…”

Section: Discussionsupporting

confidence: 92%

Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Levent,

Božić,

Petrujkić

et al. 2023

Microorganisms

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Livestock producers need new technologies to maintain the optimal health and well-being of their animals while minimizing the risks of propagating and disseminating pathogenic and antimicrobial-resistant bacteria to humans or other animals. Where possible, these interventions should contribute to the efficiency and profitability of animal production to avoid passing costs on to consumers. In this study, we examined the potential of nitroethane, 3-nitro-1-propionate, ethyl nitroacetate, taurine and L-cysteinesulfinic acid to modulate rumen methane production, a digestive inefficiency that results in the loss of up to 12% of the host’s dietary energy intake and a major contributor of methane as a greenhouse gas to the atmosphere. The potential for these compounds to inhibit the foodborne pathogens, Escherichia coli O157:H7 and Salmonella Typhimurium DT104, was also tested. The results from the present study revealed that anaerobically grown O157:H7 and DT104 treated with the methanogenic inhibitor, ethyl nitroacetate, at concentrations of 3 and 9 mM had decreased (p < 0.05) mean specific growth rates of O157:H7 (by 22 to 36%) and of DT104 (by 16 to 26%) when compared to controls (0.823 and 0.886 h−1, respectively). The growth rates of O157:H7 and DT104 were decreased (p < 0.05) from controls by 31 to 73% and by 41 to 78% by α-lipoic acid, which we also found to inhibit in vitro rumen methanogenesis up to 66% (p < 0.05). Ethyl nitroacetate was mainly bacteriostatic, whereas 9 mM α-lipoic acid decreased (p < 0.05) maximal optical densities (measured at 600 nm) of O157:H7 and DT104 by 25 and 42% compared to controls (0.448 and 0.451, respectively). In the present study, the other oxidized nitro and organosulfur compounds were neither antimicrobial nor anti-methanogenic.

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“…Short-chain nitro compounds also have shown bactericidal effects on Salmonella spp., Escherichia spp. and other gastric and intestinal bacteria (Arzola-Alvarez et al, 2016;Arzola et al, 2017;Correa et al, 2017;Ruiz-Barrera et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter

Arzola-Alavarez

Castillo-Castillo

Anderson

et al. 2020

Braz. J. Poult. Sci.

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To study the antimicrobial and uric acid-preserving activity of pine bark tannin on poultry litter composting, antibiotic-free wood chipbased poultry litter was distributed (11 g) to 50-mL conical centrifuge tubes and immediately amended with 1.3 mL0.4 M sodium phosphate buffer (control) or with 1.3 mL condensed tannins from pine bark (Pinus palustris; 9 % tannin wt/vol in water). All tubes (n = 3 tubes/treatment) were inoculated with a novobiocin and naladixic acid-resistant Salmonella typhimurium (STNN) to achieve 3.0 log 10 CFU/g and incubated at 37 o C for 3 days to simulate an initial compost period. Wildtype E. coli and the challenge STNN strain as well as concentrations of ammonia, uric acid and urea were measured on days 0 and 3. Pine bark tannin treatment decreased (p<0.01) STNN populations in the litter by 0.6 log units compared to the controls. Wildtype E. coli populations were unaffected by tannin treatment (p>0.05). Ammonia accumulation decreased (p<0.01) 23% in tannin-treated litter compared to the control (2.8 ± 0.1 µmol/g). Conversely, the residual uric acid concentration was 1.6-fold higher (p=0.02) in litter treated with the pine bark tannin than in the control litter. Urea concentrations were unaffected by tannin treatment (p>0.05). Results suggest that pine bark tannin treatment may preserve uric acid and reduce ammonia volatilization in composted litter while aiding Salmonella control.

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Effect of sole or combined administration of nitrate and 3-nitro-1-propionic acid on fermentation and Salmonella survivability in alfalfa-fed rumen cultures in vitro

Cited by 7 publications

References 45 publications

Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter

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