Effect of solid storage on caprine semen conservation at 5°C

Salvador, Ignacio Cabeza del; Yániz, J.L.; Viudes-de-Castro, M.P.; Gómez, EA; Silvestre, Mattia

doi:10.1016/j.theriogenology.2006.02.042

Cited by 42 publications

(46 citation statements)

References 30 publications

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“…The freezing process produces physical and chemical stress on the sperm membrane which in turn reduces sperm viability and fertilizing ability. The cold shock of sperm cells during the freezing-thawing process is associated with oxidative stress induced by free radicals (Salvador et al 2006;Sanacka and Kurpisz 2004). These free radicals are eliminated by antioxidant systems.…”

mentioning

confidence: 99%

Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen

2007

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Uysal O., M. N. Bucak: Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen. Acta Vet. Brno 2007, 76: 383-390. Free radicals are known to be involved in lipid peroxidation as well as DNA and sperm membrane damages that may lead to decreased sperm motility or cell death. The balance between free radical production and their detoxifi cation may be an important factor in sperm survival and function before, during and after cryopreservation. The aim of this study was to determine the effects of the addition of the antioxidants of oxidized glutathione (GSSG), bovine serum albumin (BSA), cysteine and lycopene to freezing media on the post-thawing sperm characteristics, including motility, morphology, acrosome integrity, viability and membrane integrity. A total number of 42 ejaculates were collected using the artifi cial vagina from 4 Akkaraman rams and 10 replicates of the ejaculates were diluted with a Tris-based extender containing additives and no additives as control.GSSG (5 mM), BSA (20 mg/ml), cysteine (10 mM) and lycopene (800 μg) showed more positive effects than other concentrations of the supplements and controls in protecting sperm characteristics after the freezing-thawing process (P < 0.001).Many aspects of sperm protection, e.g. sperm motility, viability and membrane stabilisation of the sperm cells during relative cryopreservation, are the key factors in determining the preservation of sperm function. The results of this study provide a new approach to the cryopreservation of sperm from rams and related breeds, and thereby contribute to the improvement of these breeds for the world sheep industry. Antioxidants, ram semen, freezing, extender

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confidence: 99%

Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen

2007

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“…In our study, this parameter was not different between the extenders. The literature suggests that changes to motility parameters are dependent on the agent used to increase the viscosity (because it determines whether liquefaction will occur at the temperature at which motility is measured), as well as the degree of viscosity (Corcini et al, 2011;Coy et al, 2009;Salvador et al, 2006;Yániz et al, 2005). Different viscous media, therefore, influence the motility characteristics of the spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…Coy, Gadea, Rath and Hunter (2009) showed that the re-suspension of boar spermatozoa in a viscous medium increased the stability of the sperm membrane, decreased reactive oxygen species production and increased the capacity of spermatozoa to penetrate oocytes in vitro. The use of gelling extenders, mostly by the use of gelatin, has proven to be effective in prolonging the preservation of rabbit Nagy, Sinkovics, & Kovacs, 2002;Rosato & Iaffaldano, 2011), ovine and caprine (Salvador, Yániz, Viudes-de-Castro, Gómez, & Silvestre, 2006) sperm in a solid state. However, it remains to be seen whether the reported benefits are also applicable to semen stored in a liquid form.…”

Section: Introductionmentioning

confidence: 99%

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

Gil¹,

Barón²,

Guerrero³

et al. 2014

JAS

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The use of several types of gelling extenders for the storage of semen from several domestic species in the solid state has been shown to have beneficial effects on some semen quality parameters. The objective of this study was to evaluate the effect of a new high-viscosity semen extender, Zoosperm ND-5 3D ® (Import-Vet, Centelles, Spain), on the the quality of boar spermatozoa at preserved at 17ºC for 7 days. Sodium alginate was used for the first time to increase the viscosity of the extender for the liquid storage of boar semen. The same extender, but without increased viscosity, was used as a control extender (Zoosperm ND-5 ® , Import-Vet, Centelles, Spain). Sixteen ejaculates from four Pietrain boars were evaluated for motility (by the CASA system), and for viability, acrosome status, plasma membrane fluidity, externalization of phosphatidylserine at the plasma membrane of the spermatozoa and mitochondrial membrane potential (by flow cytometry). In samples diluted with the Zoosperm ND-5 3D ® viscous extender, the STR (straightness) parameter and the number of progressively motile spermatozoa were higher compared to those of the non-viscous extender (p < 0.05). In addition, the number of spermatozoa with damaged acrosomes, an unstable sperm plasma membrane and externalization of phosphatidylserine at the plasma membrane was lower in samples treated with the viscous extender (p < 0.05). In conclusion, an increase in extender viscosity improves quality of boar spermatozoa following long-term storage.

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“…Various steps of cryopreservation such as processing, freezing and thawing exert physiological as well as chemical stress on the sperm membrane (Chatterjee et al, 2001), associated with an oxidative stress induced by free radical (Salvader et al,2006). Sperm cells have a high content of unsaturated fatty acids in their membrane but lack in a significant cytoplasmic component containing antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Reduced glutathione and cysteine hydrochloride on crossbred bull semen

Perumal

2012

Nature Precedings

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Effect of solid storage on caprine semen conservation at 5°C

Cited by 42 publications

References 30 publications

Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen

Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

Reduced glutathione and cysteine hydrochloride on crossbred bull semen

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