Effect of some carcinogenic and non-carcinogenic polycyclic aromatic hydrocarbons on gap junction intercellular communication in hepatoma cell cultures

Sharovskaya, Julia; Kobliakova, Ioulia; Solomatina, N. A.; Кобляков, В. А.

doi:10.1016/j.ejcb.2005.11.006

Cited by 20 publications

(8 citation statements)

References 45 publications

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“…Increased lipid peroxidation, reduced glutathione contents, increased catalase activity, decreased SOD activity, cytoplasmic retractions and fewer intercellular junctions were observed in granulosa cells exposed to Cd [62], [63], a heavy metal compound in cigarette smoke. In vitro , B[a]P was shown to inhibit gap junction formation [64], and junctions being indispensable for oocyte-granulosa cell cross-talk [65]. So we can hypothesis that cigarette smoke may have detrimental effects on oocyte through inducing oxidative stress and injuring granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Cigarette Smoke Extract on Ovulation, Oocyte Morphology and Ovarian Gene Expression in Mice

Mai

Lei

et al. 2014

PLoS ONE

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Cigarette smoking can harm fertility, but the existing research has targeted primarily on ovarian follicles, embryos or sex hormone. In this study, we tested cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression associated with inhibition of oxidative stress using C57BL/6 mice. Mice in the experimental group were administered a cigarette smoke extract (CSE) solution (2 mg/ml) orally daily, while the blank control group was given dimethylsulfoxide (DMSO). A positive control group (menadione) was used that received an intraperitoneal injection of 15 mg/kg menadione in oil solution daily. We found that the CSE group manifested a reduced diameter of zona pellucida-free oocyte (ZP-free OD) and a morphologically misshapen first polar body (PB). Our results suggest that CSE exposure is associated with a shrink size and poor quality of oocytes. Quitting smoking is a wise choice to ensure good fertility.

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Section: Discussionmentioning

confidence: 99%

The Effects of Cigarette Smoke Extract on Ovulation, Oocyte Morphology and Ovarian Gene Expression in Mice

Mai

Lei

et al. 2014

PLoS ONE

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“…In vitro, le BaP inhibe la formation des gap-junctions, jonctions indispensables au dialogue entre les cellules de la granulosa et l'ovocyte [47]. À partir de cellules de Sertoli, cellules impliqué es dans la spermatogenè se, il a é té observé que l'exposition à du cadmium entraînait une diminution des connexines, proté ines indispensables à la structure des gap-junctions [48].…”

Section: Anomalie De La Communication Ente L'ovocyte Et Son Environneunclassified

Effets du tabagisme sur la reproduction : de l’ovocyte à l’embryon (Partie I)

Dechanet¹,

Brunet²,

Anahory³

et al. 2011

Gynécologie Obstétrique & Fertilité

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“…CX proteins are targeted by chemicals including retinoids, carotinoids, chemotherapeutic agents (Trosko and Ruch, 2002); Upham and Trosko, 2009; King and Bertram, 2005), cigarette components (Upham et al , 1996; Tai et al , 2007; Upham et al , 2008; Upham and Trosko, 2009); McKarns et al , 2000) and polycyclic aromatic hydrocarbons including 7,12-dimethylbenz[a]anthracene (DMBA) (Sharovskaya et al , 2006; Ganesan and Keating, 2014; (Trosko, 1989). DMBA, liberated from burning of organic matter incineration, destroys all type of follicles in the ovaries of exposed mice and rats, leading to ovarian failure (Gelboin, 1980; Mattison and Schulman, 1980).…”

Section: Introductionmentioning

confidence: 99%

Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice

Ganesan¹,

Nteeba²,

Keating³

2015

Toxicology and Applied Pharmacology

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The ovarian gap junction proteins alpha 4 (GJA4 or connexin 37; CX37), alpha 1 (GJA1 or connexin 43; CX43) and gamma 1 (GJC1 or connexin 45; CX45) are involved in cell communication and folliculogenesis. 7,12-dimethylbenz[a]anthracene (DMBA) alters Cx37 and Cx43 expression in cultured neonatal rat ovaries. Additionally, obesity has an additive effect on DMBA-induced ovarian cell death and follicle depletion, thus, we investigated in vivo impacts of obesity and DMBA on CX protein levels. Ovaries were collected from lean and obese mice aged 6, 12, 18, or 24 wks. A subset of 18 wk old mice (lean and obese) were dosed with sesame oil or DMBA (1mg/kg; ip) for 14 days and ovaries collected 3 days thereafter. Cx43 and Cx45 mRNA and protein levels decreased (P < 0.05) after 18 wks while Cx37 mRNA and protein levels decreased (P < 0.05) after 24 wks in obese ovaries. Cx37 mRNA and antral follicle protein staining intensity were reduced (P < 0.05) by obesity while total CX37 protein was reduced (P < 0.05) in DMBA exposed obese ovaries. Cx43 mRNA and total protein levels were decreased (P < 0.05) by DMBA in both lean and obese ovaries while basal protein staining intensity was reduced (P < 0.05) in obese controls. Cx45 mRNA, total protein and protein staining intensity level were decreased (P < 0.05) by obesity. These data support that obesity temporally alters gap junction protein expression and that DMBA-induced ovotoxicity may involve reduced gap junction protein function.

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Effect of some carcinogenic and non-carcinogenic polycyclic aromatic hydrocarbons on gap junction intercellular communication in hepatoma cell cultures

Cited by 20 publications

References 45 publications

The Effects of Cigarette Smoke Extract on Ovulation, Oocyte Morphology and Ovarian Gene Expression in Mice

The Effects of Cigarette Smoke Extract on Ovulation, Oocyte Morphology and Ovarian Gene Expression in Mice

Effets du tabagisme sur la reproduction : de l’ovocyte à l’embryon (Partie I)

Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice

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