Effect of Some Physical Factors on Lipase Production by Some Selected Fungi

Abstract

Seven fungi have been experimentally screened to get the most active two lipase producers. The experimental conditions were Czapek's yeast extract tributyrin emulsion (CYET) liquid medium, incubated at 30 °C &pH 5.0 for 8 days. Classical kinetic technique of enzymatic analysis has been used to study the effect of physical factors (pH, temperature &incubation period). Optimal yield of lipase produced by Aspergillus niger after 8 days of incubation at 30 °C &pH 5.0, while optimal yield of lipase produced by Rhizopus nigricans after 6 days of incubation at 30 °C &pH 6.0. Conclusively, from this study, it could be concluded that the optimal yield of lipase was produced by Aspergillus niger at pH 5 and 30 °C for 8 days incubation, while the optimal yield of lipase produced by Rhizopus nigricans at pH 6 and 30 °C for 6 days incubation.