Effect of some rhizosphere bacteria on root-knot nematodes

AbdelRazek, Ghena Mamdouh; Yaseen, Rabaa

doi:10.1186/s41938-020-00340-y

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…S. sciuri SsHRh3, B. pumilus BpHRh5, P. megaterium PmHRh10 from HRh and M. laevaniformans MlIRh3, S. kloosii SkIRh4 and P. aryabhattai PaIRh7 from IRh were predominately associated with nematode antagonism in the current study. Diseasesuppressive microorganisms have been isolated from the rhizosphere soil of nematode-infected [17] and uninfected hosts [18]. We found nematode antagonistic rhizobacteria from both HRh and IRh.…”

Section: Discussionmentioning

confidence: 88%

Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation

et al. 2023

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Intermingled uninfected and root‐knot nematode‐infected tomato plants are commonly observed under protected cultivation. To understand the role of rhizobacteria underlying the susceptibility to nematode infectivity in these tomato plants, 36 rhizobacteria (18 from each type) with morphologically distinct colony characteristics were isolated from the rhizosphere of uninfected and root‐knot nematode‐infected tomato plants. The in vitro nematicidal potential of rhizobacteria from the uninfected rhizosphere was significantly higher than that from the infested rhizosphere. The three most effective antagonists were identified as Microbacterium laevaniformans, Staphylococcus kloosii, Priestia aryabhattai from root‐knot‐nematode‐infected tomato rhizosphere and Staphylococcus sciuri, Bacillus pumilus, and Priestia megaterium from the rhizosphere of uninfected tomato. Volatile organic compounds from these rhizobacteria were characterized. Except for S. kloosi, the soil drenching with other rhizobacteria significantly reduced juvenile penetration (>60%) in tomato roots. Furthermore, the application of a single or consortium of these rhizobacteria affected nematode reproduction in tomato. Four consortia of rhizobacteria (S. sciuri + B. pumilus + P. megaterium), (B. pumilus + P. megaterium), (S. sciuri + B. pumilus), and (S. sciuri + P. megaterium) from uninfested rhizosphere and two consortia (M. laevaniformans + P. aryabhattai), (M. laevaniformans + S. kloosii + P. aryabhattai) from infested rhizosphere (IRh) effectively reduced M. incognita reproduction and considerably enhanced plant growth and yield in tomato. The nematicidal efficacy, however, decreased when S. kloosii was applied in the consortium. These distinctive effects illustrate how the plant susceptibility to nematode infectivity is modulated under natural conditions.

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Section: Discussionmentioning

confidence: 88%

Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation

et al. 2023

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“…Efficient bacterial strains of fluorescent Pseudomonads habitat rhizospheric soils of chilli, field bean, green gram, brinjal, sunflower, red gram, groundnut, tomato, bermuda grass, beans, sorghum, paddy and sesame ( Manjunatha et al, 2012 ). Furthermore, bacterial strains were reported in the rhizosphere of soils with nematode outbreaks ( AbdelRazek & Yaseen, 2020 ). The efficient bacterial and fungal microbial strains, later to invitro tests, through 16S RNAwere identified to be B. subtilis ( ), Bacillus velezensis ( ), Alcaligenes faecalis ( ), Rhizobium pusense ( ), Trichoderma asperellum ( ) and T. allahabadensis ( ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

Jena,

Choudhury,

Das

et al. 2023

PeerJ

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Meloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematode (M. graminicola). Eighteen bacterial strains and eleven fungal strains were isolated from the rhizosphere of crops like rice, okra, ash gourd, chili, beans and cucumber, enveloping diverse soil types from the Upper Brahmaputra Valley region of Assam. Six bacterial strains were gram-positive according to morphological results, while twelve others stained negatively. Fifteen bacteria were rod-shaped, two were coccus and one was diplococcus, and all the bacterial isolates showed signs of movement. All the bacterial strains exhibited positivity for gelatin hydrolysis and catalase test. Seven bacteria showed positive, while eleven showed negative reactions to possess the ability to deduce carbon and energy from citrate. The study of the in vitro efficacy of the twenty-nine bacterial and fungal isolates tested against second-stage juveniles (J2) of Meloidogyne graminicola revealed that all the bacterial and fungal isolates potentially inhibited the test organism and caused significant mortality over sterile water treatment. The promising bacterial and fungal isolates that exhibited mortality above 50% were identified as BSH8, BTS4, BTS5, BJA15, FJB 11 and FSH5. The strain BSH8 exhibited the best result of mortality, with 80.79% mortality against J2 of M. graminicola. The strain BTS4 and BTS5 expressed mortality of 71.29% and 68.75% under in-vitro conditions and were significant. The effective and promising bioagents were identified using the 16 S rRNA sequencing as Bacillis subtilis (BSH8), Bacillus velezensis (BTS4), Alcaligenes faecalis (BTS5), Rhizobium pusense (BJA15), Talaromyces allahabadensis (FSH5) and Trichoderma asperellum (FJB11). These results indicated the microorganism’s potential against M. graminicola and its potential for successful biological implementation. Further, the native strains could be tested against various nematode pests of rice in field conditions. Its compatibility with various pesticides and the implication of the potential strains in integrated pest management can be assessed.

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“…The chemical nematicide ethoprophos Smart-N (UPI CropScience Co., Ltd., Tianjin, China) was purchased from the local supplier Starchem Industrial Chemicals, 6th of October City, Egypt. A wild-type P. stutzeri strain BB19 previously isolated and identified from bean plants in the Baloza area, North Sinai Governorate, Egypt [22], and it was utilized to synthesize BioSeNPs in the present study. P. stutzeri cells were routinely grown in Luria-Bertani (LB) broth medium [23] at 28 • C. The culture of M. incognita was isolated from the okra plant grown in a greenhouse at El-Behera Governorate, Egypt.…”

Section: Chemicals Bacterial and Nematode Strains And Culture Conditionsmentioning

confidence: 99%

Effect of Biosynthesized Nanoselenium on Controlling Tomato Root-Knot Nematode Meloidogyne incognita

Daoush,

Hendawey,

Yaseen

et al. 2023

Agronomy

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Tomato is a globally important fruit crop, which is easily susceptible to infection by plant-parasitic nematodes during growth. In this study, selenium nanoparticles were biosynthesized through the selenite reduction pathway in a wild-type Pseudomonas stutzeri BB19 and were characterized by uniform nanostructured needle-like forms with an average size of 95.2 nm. The nematicidal effect of biosynthesized selenium nanoparticles (BioSeNPs) at different concentrations (2, 6, and 10 ppm) during the pre- or post-infection of tomato root-knot nematode (RKN), Meloidogyne incognita, was assessed under greenhouse conditions. The BioSeNPs were applied as a foliar spray or a soil drench, compared to ethoprophos nematicide (100 mL/pot). The results showed that the ethoprophos nematicide significantly reduced the number of galls, egg mass, and eggs/egg mass of M. incognita by 94.2, 92.8, and 49.8%, respectively. BioSeNPs, as foliar sprays, significantly reduced the number of galls in post-treated infected tomato plants at 10 ppm and in pre-treated infected tomato plants at 2 ppm by 91.9 and 91.4%, respectively. Concerning the egg mass, BioSeNPs significantly reduced it in pre-treated infected tomato plants at 2 ppm as a foliar spray by 90.9%. Moreover, BioSeNPs significantly reduced the eggs/egg mass in pre-treated infected tomato plants at 2 ppm as a soil drench by 43.3%. On the other hand, the BioSeNPs considerably improved tomato growth, chlorophyll a and b, carotenoid content, and enzymes (i.e., catalase and peroxidase) activity compared to untreated infected tomato plants (negative control). Hence, the BioSeNPs show a significant application potential as a cost-effective and environmentally friendly biocontrol agent for RKN management in tomato plants.

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Effect of some rhizosphere bacteria on root-knot nematodes

Cited by 8 publications

References 25 publications

Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation

Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

Effect of Biosynthesized Nanoselenium on Controlling Tomato Root-Knot Nematode Meloidogyne incognita

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