Effect of Some Teeth Cleaning Methods on the Microbiological Content of the Oral Cavity

Abstract

The study was conducted in the period from January to May 2018. The study included the identification of the total account and types of microbial content of the oral cavity during the normal daily practices at different times, which were after waking up, after washing mouth with water (rinsing), after breakfast and after using toothpaste for 33 donors of females and males, smokers and non-smokers, and the ages range were 25-35 years. The results of the study showed that all donor groups showed positive growth during the isolation stages, but there were no significant differences in the growth density of the total count, Enterobacteriaceae and staphylococcus for the donor groups, and the recorded account are not considered to be dangerous to the health of mouth. This is because donor's mouth health was good and smoking period was short. The highest account of bacteria in isolates were seen among smokers after waking up was 74-550 cfu / ml. The study also included the diagnosis of isolated microbial species using VITEK2. The results showed that the isolated bacterial species are Staphylococcus aureus, Lelliottia amnigena, Pseudomonas fluorescens, Pseudomonas luteola, Klebsiella pneumonie, Stenotrophomonas maltophilia which were varied in count, Bacterial resistance was tested for some of the antibiotics that were Doxycycline (DO), Mastiscs (TS), Ceftriaxone (CRO), Tobramycine (TOB), Mastiscs (T), Mastdiscs (T), Gentamicin (CN). The results showed that L. amnigena and S.maltophilia were sensitive to all antibiotics. P. fluorescens were sensitive to antibiotics CN, T, TOB, DO and resistance to antibiotics CRO, TS whereas K.pneumonie was sensitive to all Antibiotics used except the DO antibiotic. The S.aureus bacteria showed resistance to antibiotics CN, TOB, CRO, TS and sensitive to antibiotics T, DO.