Effect of soyabean meal, rapeseed meal and extruded field bean seeds on duodenal amino acids supply in sheep

Abstract

In the first of two experiments, four rumen cannulated wethers were used to determine the extent of rumen protein degradation in soyabean meal (SBM), rapeseed meal (RSM) and extruded field bean seeds (EFB), using the in sacco method. Rumen non-degradable N in SBM, RSM and EFB of total-N was 38, 29 and 48%, respectively. In the second experiment, four rams with cannulas in the duodenum were fed isoenergetic total mixed rations (TMR) containing similar amounts of protein truly digestible in the small intestine (PDI). TMR consisted of meadow hay and concentrate (50:50, DM basis) in which SBM, SBM+RSM and SBM+EFB were the main sources of protein.The highest, statistically significant (P<0.05) methionine and cystine concentrations in the duodenal digesta were found in sheep fed SBM+RSM diet, while the lowest -in those fed SBM+EFB diet. Significantly higher (PO.05) flows of total N (33.49 g/day), non-ammonia N (33.08 g/day) and total amino acid (197.7 g/d) were observed in sheep fed rations with SBM + EFB.

Effect of duodenal infusion of methionine on milk yield and composition in ewes at late lactation fed hay-based diet

“…In an earlier study (Urbaniak et al, 2001) in which this experimental diet was tested, it was found that Met and Lys concentration in total AA passing to the small intestine of sheep amounted to, 1.67 and 6.88%, respectively. Duodenal infusion of 2, 4 and 6 g/d of Met resulted in an increase of absorbable Met concentration which amounted to 3.15, 4.60 and 6.00%, respectively.…”

“…The composition of the concentrate, TMR chemical composition and nutritive value are given in Table 1. In an experiment carried out earlier (Urbaniak at al., 2001) the effect of the above mentioned TMR on AA profile and AA flow into the small intestine of sheep was determined. Daily amounts of DL-methionine were disolved in 240 ml of physiological salt solution, and infused into the duodenum via a peristaltic pomp for 6 h at a rate of 40 ml/h.…”

“…Ewes were milked at 07.00 and 16.00 h. For the last two days of each experimental period the feed intake and refusals as well as lactational performance were recordered. Samples from morning and afternoon milkings were pooled according to yield and analysed for protein, fat and lactose contents by infrared analysis (Milkoscan 133B, Foss Electric, Denmark) and for amino acids using an automatic amino acid analyser (T-339, Microtechna, Czech Republic) according to methods given by Urbaniak et al (2001). Casein was determined according to the method given by Jurczak (1999).…”

Supplementation of mixed roughage-based diet with rumen protected methionine for lactating goats

“…Animals were fed and milked twice daily at 7.00 and 16.00. In the last day of experimental period samples from morning and afternoon milkings were pooled according to yield and analysed for protein, fat and lactose contents by infrared analysis (Milkoscan 133B, Foss Electric, Denmark) and for amino acids using an automatic amino acid analyser (T-339, Microtechna, Czech Republic) according to methods given by Urbaniak et al (2001). Casein was determined according to the method given by Jurczak (1999).…”