Effect of Soybean Lecithin-Based Semen Extender on Freezability and Fertility of Rahmani Ram Spermatozoa

Khalifa, E. I.; Abdel-Hafez, M. A. M.

doi:10.12816/0005054

Cited by 9 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lecithin is a valuable plant origin to prevent sperm cold shock, because it consists of glycerol, two fatty acids, phosphate group and choline which play an important role in protecting sperm cell membrane. The previous facts has been developed and utilized to preserve spermatozoa of human (Jeyendran et al, 2008), domestic cat (Vick et al, 2010), stallion (Papa et al, 2011), ram (Khalifa and Abdel-Hafez, 2014), buffalo (Akhter et al, 2012), goat (Chelucci et al, 2015) and bovine bull (El-Sisy et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

El-Harairy

Khalil

Khalifa

et al. 2018

Journal of Animal and Poultry Production

View full text Add to dashboard Cite

This study designed to examine effects of adding different concentrations of propolis ethanolic extract (PEE) as antioxidant to ram semen extenders; tris egg yolk (TEY) or tris-soybean lecithin (TSBL) on sperm characteristics, lipid peroxidation and enzymatic activities in seminal plasma of ram semen preserved at 5°C for 48 hours. Semen was collected from five adult Rahmani rams using an artificial vagina twice/week for 5 weeks. Pooled semen was extended with TEY or TSBL supplemented with different concentrations from PEE (0, 0.1, 0.5 and 1.0 mg/ml). Polyphenols and flavonoid compounds in propolis ethanolic extract were identified. In each treatment, percentage of progressive motility, livability, and abnormality were determined. Also, percentages of sperm membrane integrity or chromatin damage were determined. Concentrations of total antioxidant (TAO) and malondialdehyde (MDA), and activity of acid phosphatase (ACP), lactate dehydrogenase (LDH), and asprtate (AST) and alanine (ALT) transaminase, were determined. Results showed that the most effective polyphenolic compounds in the prolopolis ethanolic extract was the chlorogenic acids, catechein, protocatchuic and pyrogallol (120.623, 53.006, 23.907 and 11.048 mg/100g extract, respectively). Luteo.6-arbinose 8-glucose was the predominant identified flavonoid component in propolis extract (5268.78ppm), followed by acacetin, hisperidin, apig.6-rhamnose8glucose, apig.6-glucose8-rhamnose, kamp.3-(2-p-comaroyl) glucose and luteo.6-glucose 8-arbinose with concentrations (647. 53, 561.09, 366.47, 150.46, 144.51 and 109.42 ppm, respectively. The effect of propolis ethanolic extract supplementation to ram semen extenders was significant (P<0.05) only on the overall percentages of sperm progressive motility and sperm chromatin damage while, sperm livability, abnormality and membrane integrity were not affected significantly (P<0.05). Concentration of TAC was numerically (P= 0.82) higher in TEY extender with different concentrations of PEE than TSBL. However, MDA was lower (P<0.05) in free TEY, followed by TSBL with PEE. Activity of LDH, AST and ALT tended to be lower in seminal plasma of semen extended with TSBL than TEY. These results showed beneficial effects of soybean lecithin and propolis ethanolic extract in tris based extender on progressive motility and chromatin integrity of spermatozoa up to 48 h of preservation at 5°C. Also, lipid peroxidation and enzyme activity were lower in TSBL than TEY. In addition, soybean lecithin can effectively replace egg yolk as a protective additive with propolis ethanolic extract for preservation extender without any detrimental effects on post-chilling semen quality in rams.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

El-Harairy

Khalil

Khalifa

et al. 2018

Journal of Animal and Poultry Production

View full text Add to dashboard Cite

show abstract

“…The 1st hypothesis found that phospholipids being the major component of the sperm cell membrane which is played an important physiological function in reducing the cooling point and in minimizing the replacement of plasmalogen to reduce the possible mechanical damage to the sperm cell membrane (Gamal et al., 2016). Accordingly, exogenous phospholipids present in EGD and SLD (Khalifa & Abdel‐Hafez, 2014) extenders can supply some of the sperm cells membrane phospholipids to maintain plasma membrane structure and function. The 2nd hypothesis has a widely accepted, it found that phospholipids in either egg yolk or soybean lecithin do not enter the sperm cells membrane to alter the phospholipids membrane concentration but could be formed a protective film around the sperm cells membrane to prevent deterioration of sperm membrane, intracellular fluidity and could protect the sperm cells membrane from mechanical damage during temperature variation (Nguyen, Ponchunchoovong, Kupittayanant, & Kupittayanant, 2019).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The possibility of using citric phosphate dextrose in chilling ram semen instead of egg yolk and soybean lecithin to improve fertility

Mohamed

Mahdy

Khalifa

2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

Artificial insemination (AI) is one of the important tools for genetic improvement, and it is widely based on stored semen for future use. The quality of stored semen is affected by handling procedures such as dilution, centrifugation storage and freezing (Khalifa, Abdel-Hafez & Ghobashy, 2016; Khalifa, Mohamed, & Abdel-Hafez, 2016). The diluents used for survival spermatozoa should have adequate pH and buffering capacity, suitable osmolality and should protect spermatozoa from cryogenic damages (Sharafi, Zhandi, & Sharif, 2015). It is suggested that the extender type used to dilution semen is an important and effective factor in the successful storage of spermatozoa

show abstract

“…Artificial insemination using chilled and frozen semen can accelerate animal production in breeder farms, simultaneously increasing genetic progress. Indeed, numerous studies have already demonstrated the efficiency of artificial insemination in small ruminants [4][5][6][7][8][9][10][11]. In a comparative study between extenders for freezing semen, a pregnancy rate of 54.55-63.64% was found in Rhamani ram [4], a lambing rate of 56.7-64.5% in Suffolk ewes [5], and a pregnancy rate of 46.2-71% of in Chios rams [6].…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, numerous studies have already demonstrated the efficiency of artificial insemination in small ruminants [4][5][6][7][8][9][10][11]. In a comparative study between extenders for freezing semen, a pregnancy rate of 54.55-63.64% was found in Rhamani ram [4], a lambing rate of 56.7-64.5% in Suffolk ewes [5], and a pregnancy rate of 46.2-71% of in Chios rams [6]. In goats, depending on the insemination protocol, fertility varied from 30.3% to 52.5% [7], 38-50% according to the frozen semen type used [8], 35-65% by the synchronization method used [9], 62-64.3% depending on the number of inseminations done [10], and 46-75% per time taken to inseminate a goat [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Akourki

Arantxa²,

Perdomo³

et al. 2021

Vet World

View full text Add to dashboard Cite

Background and Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed. Materials and Methods: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen. Results: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell. Conclusion: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris–egg yolk–based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.

show abstract

Effect of Soybean Lecithin-Based Semen Extender on Freezability and Fertility of Rahmani Ram Spermatozoa

Cited by 9 publications

References 20 publications

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

The possibility of using citric phosphate dextrose in chilling ram semen instead of egg yolk and soybean lecithin to improve fertility

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Contact Info

Product

Resources

About