An enhanced combustion concept, pre-chamber jet ignition can provide high ignition energy and a wide dispersion of ignition sources, thus improving combustion performance and realizing stable lean combustion. However, the ignition performance of the pre-chamber jets depends strongly on the pre-chamber combustion process and is sensitive to many factors. Therefore, this paper will explore the impact of spark timing on the pre-chamber combustion, turbulent jet characteristics, and main chamber combustion performance by CFD simulation based on an active pre-chamber natural gas engine. The in-cylinder flow and combustion processes of the engine are calculated with the fuel-air equivalence ratio of the main chamber maintained at 0.5, and the spark timing varied from 684°CA to 708°CA with a step of 3°CA (compression top dead center is 720°CA). The results show that, in the present engine operating point, the pre-chamber combustion heat release rate is improved when the spark timing is retarded from 696°CA to 705°CA, and the injection velocity of the pre-chamber jet accordingly increases. As the spark timing continues to be delayed until 708°CA, the pre-chamber combustion deteriorates, and the injection velocity of the pre-chamber jet decreases. Under the combustion phase CA50 no more than 725°CA and the combustion duration CA5-90 fixed around 30°CA, the IMEP will maintain a relatively stable value when the spark timing is delayed from 693°CA to 705°CA, in the meantime, the NOx emission decreases with the delay of spark timing. Therefore, on the premise of ensuring engine power performance, it is a feasible scheme to delay spark timing to reduce NOx emission for the natural gas engine with an active pre-chamber.