Effect of Sperm Chromatin Damage on Fertilization Ratio and Embryo Quality Post-Icsi

Abstract

A total of 56 semen samples was collected from men in the ICSI program. The female partners of all men were under 35 years of age. Sperm chromatin integrity was evaluated by staining the spermatozoa with acridine orange. > 56% red fluorescence was taken as abnormal chromatin status. A significant negative association was found between the percentage of sperm with DNA fragmentation and fertilization rate. Increased red fluorescence indicated impaired fertilization outcome. Good quality embryo rates were significantly lower in abnormal AO stained group. Chromatin damage precedes the loss of fertilization potential and poor embryo quality.

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

“…Although preservation of sperm membranes and motility are important measures of sperm quality, they may not reveal the changes in DNA. The analysis of DNA fragmentation (sDF) has explained the infertility of individuals with apparently normal seminograms [24,25]. This approach is particularly interesting because DNA fragmentation correlates poorly with classical parameters of semen quality [26] as also occurs in donkey sperm [27].…”

Role of male factor in early recurrent embryo loss: do antioxidants have any effect?

“…Increased red fluorescence indicated impaired fertilization outcome, and good quality embryo rates were significantly lower in the group with high sperm DNA fragmentation. Chromatin damage precedes the loss of fertilization potential and poor embryo quality, resulting in pregnancy loss (54)(55)(56)(57)(58)(59).…”

The clinical significance of sperm DNA damage detection combined with routine semen testing in assisted reproduction

“…It has been used to evaluate relationships between abnormal sperm DNA detected by AO and adverse outcomes in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), such as lower implantation rates and higher pregnancy loss. It has even been suggested that abnormal sperm DNA may affect offspring (7)(8)(9).…”