Effect of spermidine on radiation-induced long-term bone marrow cell injury

Guan, Bowen; Li, Chengcheng; Yin, Yong; Lü, Yanhua; Sun, Yuhang; Su, Lulu; Shi, Guiying; Bai, Lin; Liu, Jiangning; Meng, Aimin

doi:10.1016/j.intimp.2022.109557

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…The CFU‐GEMM results, which reflect HSC function, are more responsive to the sensitivity of different strains, suggesting that HSCs are more susceptible to BU compared with progenitor cells, which is consistent with the results of the previous experiments (Figure 1 ). 13 …”

Section: Resultsmentioning

confidence: 99%

“…In vitro, colony‐forming ability assay was performed as previously described. 13 Briefly, mouse bone marrow mononuclear cells (BMMNCs) were collected aseptically, and the concentration was adjusted to 4 × 10 5 cells/ml with PBS. The cells were mixed with a methylcellulose‐based medium (MethoCult GF M3434, STEMCELL Technologies) and inoculated into 24‐well plates.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro, colony‐forming ability assay was performed as previously described 13 . Briefly, mouse bone marrow mononuclear cells (BMMNCs) were collected aseptically, and the concentration was adjusted to 4 × 10 5 cells/ml with PBS.…”

Section: Methodsmentioning

confidence: 99%

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Screening for urinary markers predicting hematopoietic stem cell injury induced by busulfan using genetically diverse mice

Sun

Guan

Liu

et al. 2023

Anim Models and Exp Med

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Background Busulfan (BU) is an alkylating agent used as a conditioning agent prior to hematopoietic stem cell (HSC) transplantation as it is known to be cytotoxic to host hematopoietic stem and progenitor cells. The susceptibility of HSCs to BU injury plays an important role in the myeloablative efficacy of BU. Different susceptibilities were demonstrated in genetically diverse (GD) mice in our preliminary research. Methods Three strains of GD mice with different susceptibilities to BU‐induced HSC injury were used for screening biological markers of HSC injury susceptibility in urine. The urine proteins were analyzed using liquid chromatography coupled with tandem mass spectrometry to screen for differentially expressed proteins. Screening for possible biomarkers based on differences in protein expression abundance was validated using enzyme‐linked immunoassay (ELISA). Results Functional analysis showed that the differential proteins were all involved in a series of biological pathways related to cellular senescence, apoptosis, and angiogenesis; whereas the differential proteins of the high‐susceptible strain were enriched for the regulation of bone marrow microenvironment pathways, those of low‐susceptible strain were enriched for the proapoptotic effect of GTPase pathways. Based on protein abundance differences, several urinary proteins that may be indicative of susceptibility were screened, and ELISA validation results showed that angiotensin‐converting enzyme may be a potential biomarker predicting HSC susceptibility for BU conditioning. Conclusions This study indicates that urinary protein levels can reflect differences in susceptibility to BU‐induced HSC injury. Using GD mice to construct genetic difference models will provide preclinical data for screening BU‐related biological markers.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%