Effect of spider venom on inhibition proliferation of <scp>TE13</scp> cells <i>in vivo</i> and <i>in vitro</i>

Gao, Li; Zhang, Jing; Li, Xinyan; Zhao, Min; Li, Lijun; Liu, Xin; Zhao, Bin

doi:10.1111/1759-7714.12020

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…As presented in a previous study, flow cytometric analysis of TE13 and HeLa cells treated with crude M. raveni spider venom revealed that the main mode of cell death was necrosis, with minimal apoptotic cells. The researchers eventually concluded that apoptosis, necrosis, and cell lysis of tumors are the possible mechanisms by which the crude venom of M. raveni inhibited tumor growth [20]. It is therefore important to identify the responsible spider venom component and to determine the proper concentration in order to elicit the desired mode of cancer cell death.…”

Section: Discussionmentioning

confidence: 99%

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

Mayor

Guevarra

Santiago-Bautista

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Spider venom is a potential source of pharmacologically important compounds. Previous studies on spider venoms reported the presence of bioactive molecules that possess cell-modulating activities. Despite these claims, sparse scientific evidence is available on the cytotoxic mechanisms in relation to the components of the spider venom. In this study, we aimed to determine the cytotoxic fractions of the spider venom extracted from Phlogiellus bundokalbo and to ascertain the possible mechanism of toxicity towards human lung adenocarcinoma (A549) cells. Methods: Spider venom was extracted by electrostimulation. Components of the extracted venom were separated by reversed-phase high performance liquid chromatography (RP-HPLC) using a linear gradient of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in 95% acetonitrile (ACN). Cytotoxic activity was evaluated by the MTT assay. Apoptotic or necrotic cell death was assessed by microscopic evaluation in the presence of Hoechst 33342 and Annexin V, Alexa Fluor TM 488 conjugate fluorescent stains, and caspase activation assay. Phospholipase A 2 (PLA 2) activity of the cytotoxic fractions were also measured. Results: We observed and isolated six fractions from the venom of P. bundokalbo collected from Aurora, Zamboanga del Sur. Four of these fractions displayed cytotoxic activities. Fractions AT5-1, AT5-3, and AT5-4 were found to be apoptotic while AT5-6, the least polar among the cytotoxic components, was observed to induce necrosis. PLA 2 activity also showed cytotoxicity in all fractions but presented no relationship between specific activity of PLA 2 and cytotoxicity. Conclusion: The venom of P. bundokalbo spider, an endemic tarantula species in the Philippines, contains components that were able to induce either apoptosis or necrosis in A549 cells.

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Section: Discussionmentioning

confidence: 99%

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

Mayor

Guevarra

Santiago-Bautista

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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“…In vivo testing revealed that the tumor size was significantly decreased after 21 days of treatment with the venom. 32 2.1.2. Peptides targeting cancer cells.…”

Section: Spider Venoms In Biomedicinementioning

confidence: 99%

Anticancer, antimicrobial, and analgesic activities of spider venoms

Akef

2018

Toxicology Research

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Spider venoms are complex mixtures composed of a variety of compounds, including salts, small organic molecules, peptides, and proteins. But, the venom of a few species is dangerous to humans. High levels of chemical diversity make spider venoms attractive subjects for chemical prospecting. Many spider venom components show potential activity against a wide range of human diseases. However, the development of novel venom-derived therapeutics requires an understanding of their mechanisms of action. This review will highlight the structures, activities and the possible mechanisms of action of spider venoms and their components against cancer, microbial infections, and pain.

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Anticancer Potential of Spider Venom

2016

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Effect of spider venom on inhibition proliferation of TE13 cells in vivo and in vitro

Abstract: Background: The aim of this study was to evaluate the cytotoxic and antitumor activity of spider venom (SV). Methods: Cell proliferation and cytotoxicity were determined by

Cited by 4 publications

References 16 publications

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

Anticancer, antimicrobial, and analgesic activities of spider venoms

Anticancer Potential of Spider Venom

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