Effect of Splenectomy and Autologous Spleen Transplantation on the Serum Platelet-Activating Factor Acetylhydrolase (PAF-AH) Activity and Acute Phase Response (APR) in a Porcine Model

Abstract

The aim of this study was to evaluate the inflammatory response after total splenectomy and spleen autotransplantation in a porcine model by measuring serum platelet-activating factor acetylhydrolase activity, C-reactive protein and albumin concentrations. Nineteen piglets were used in the experiment. After induction of anesthesia, animals were randomly divided into three groups: sham-operation with spleens intact (n = 6), total splenectomy (n = 6), and splenic autotransplantation (n = 7) with small fragments of the spleen autotransplanted into the greater omentum. The blood samples were taken just before surgery and on day 1st, 5th, 12th, 26th and 40th postoperatively. PAF-AH activity, CRP and albumin concentrations were assayed in the sera. After total splenectomy, PAF-AH activity was significantly increased on day 5th, while there was no significant increase after spleen autotransplantation or the sham-operation. CRP was significantly increased after surgery in all experimental groups. Albumin was significantly decreased after surgery from day 5th until day 40th in splenectomized and autotransplanted pigs. Increased PAF-AH activity after splenectomy and spleen autotransplantation might be attributed to inflammatory conditions due to the loss of splenic tissue and trauma. Time-course increase of CRP, in all groups after surgery suggests post-injury inflammatory response due to tissue lesion during operation.

Risk factors for thromboembolism and pulmonary artery hypertension following splenectomy in children with hereditary spherocytosis

“…A value between 3 and 10 mg/L, in an otherwise asymptomatic person is predictive of increased cardiovascular risk [31]. CRP has been reported to elevate significantly following splenectomy in porcine model [32]. In a study of 20 splenectomized patients by Sedlacek et al [33] CRP was significantly elevated in patients splenectomized for hematological indications as compared to post-traumatic splenectomy (P < 0.05).…”

“…These have been recognized as risk factors for cerebrovascular and coronary heart disease. Pediatr Blood Cancer 2014;61: [29][30][31][32][33]. # 2013 Wiley Periodicals, Inc.…”

Effects of in-feed zeolite treatment on some biomarkers in dairy cows with subclinical mastitis

“…Research in domestic animals led to the identification of several APPs previously described in humans and in laboratory animals, and differences in the acute-phase response of individual APPs between different animal species were observed [9,10]. C-reactive protein is the main protein of the acute phase in humans, dogs, and pigs, while in ruminants its level does not increase significantly during infections or inflammation [10][11][12][13][14][15].…”