Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury

Liu, Xingyu; Sun, Yuhua; Li, Huaiqin; Li, Yuting; Li, Meiyuan; Yuan, Ying; Cui, Shusen; Yao, Dengbing

doi:10.1186/s12868-017-0348-1

Cited by 22 publications

(31 citation statements)

References 41 publications

(30 reference statements)

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“…These provided the scienti c basis for our following experiment and also provided a theoretical foundation for the clinical transformation of experimental research. Intriguingly, we found that the expression pattern of SPP1 and PKCα after median nerve injury in clinical cases was a little bit different from the performance of the injured sciatic nerve injury in rats [23,24], but the overall upward trend was consistent. This suggests that there are differences between human and rat, which should be considered in future studies.…”

Section: Discussionmentioning

confidence: 73%

“…As both SPP1 and PKCα promoted cell proliferation and inhibited cell apoptosis of SCs, and silence of SPP1 resulted in the reduced PKCα [23], we proposed that SPP1 might function through PKCα. To check this hypothesis, we rstly introduced si-SPP1 to knockdown and GV146-SPP1 to overexpress of SPP1 in primary cultured SCs.…”

Section: Spp1 Promoted Sc Proliferation and Inhibited Apoptosis Throumentioning

confidence: 88%

“…Our previous study suggested that the expression of SPP1 was up-regulated after sciatic nerve injury in rats, and elevated SPP1 promoted the proliferation and inhibited the apoptosis of SCs [23]. However, the underlying mechanism is largely unclear.…”

Section: Introductionmentioning

confidence: 98%

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SPP1 Promotes Schwann Cell Proliferation and Survival through PKCα by Binding with CD44 and αvβ3 after Peripheral Nerve Injury

Wang

Zhang

et al. 2020

Preprint

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Abstract Background: Schwann cells (SCs) play a crucial role in Wallerian degeneration after peripheral nerve injury. The expression of genes in SCs undergo a series of changes, which greatly affect the proliferation and apoptosis of SCs as well as the fate of peripheral nerve regeneration. However, how do these genes regulate the proliferation and apoptosis of SCs remains unclear. Results: SPP1 and PKCα were found upregulated after human median peripheral nerve injury, which promoted SCs proliferation and survival. The promoted proliferation and inhibited apoptosis by SPP1 were blocked after the treatment of PKCα antagonist Gö6976. Whereas, the inhibited proliferation and enhanced apoptosis induced by silence of SPP1 could be rescued by the activation of PKCα, which suggested that SPP1 functioned through PKCα. Moreover, both CD44 and αvβ3 were found expressed in SCs and increased after peripheral nerve injury. Silence of CD44 or β3 alleviated the increased proliferation and inhibited apoptosis induced by recombinant osteopontin, suggesting the function of SPP1 on SCs were dependent on CD44 and β3. Conclusion: These results suggested that SPP1 promoted proliferation and inhibited apoptosis of SCs through PKCα signaling pathway by binding with CD44 and αvβ3. This study provides a potential therapeutic target for improving peripheral nerve recovery.

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Section: Discussionmentioning

confidence: 73%

Section: Spp1 Promoted Sc Proliferation and Inhibited Apoptosis Throumentioning

confidence: 88%

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SPP1 Promotes Schwann Cell Proliferation and Survival through PKCα by Binding with CD44 and αvβ3 after Peripheral Nerve Injury

Wang

Zhang

et al. 2020

Preprint

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“…All animals underwent surgery to establish the sciatic nerve injury model as previously described (Liu et al, 2017 ). We randomly divided the rats into six groups and performed the operation (Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury

Sun

Cai

et al. 2018

Front. Mol. Neurosci.

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Wallerian degeneration (WD) is associated with changes in the expression levels of a large number of genes. However, the effects of these up- or down-regulated genes are poorly understood. We have reported some key factors that are differentially regulated during WD in our previous research. Here, we explored the roles of Fas ligand gene (Faslg) in WD after rat sciatic nerve injury. The data showed that Faslg was up-regulated in injured nerves. Expression changed of Faslg in Schwann cells (SCs) resulted in alterations in the release of related factors. Silencing or overexpression of Faslg affected SC proliferation, migration, and apoptosis through β-catenin, nuclear factor-κB (NF-κB), and caspase-3 pathways in vivo and in vitro. Our data suggest that Faslg is a key regulatory gene that affects nerve repair and regeneration in peripheral nerve injury. This study sheds new light on the effects of Faslg on peripheral nerve degeneration and/or regeneration.

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“…The immune microenvironment of injured nerves plays a critical role in the regulation of nerve regeneration after injury 5,6 . Debris resulting from degenerated axons and myelin can inhibit axonal regrowth and should be cleared to create a favorable microenvironment for subsequent nerve regeneration 7 .…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging of enhanced nerve repair with mesenchymal stem cells combined with microenvironment immunomodulation in neurotmesis

et al. 2020

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Introduction:The immuno-microenvironment of injured nerves adversely affects mesenchymal stem cell (MSC) therapy for neurotmesis. Magnetic resonance imaging (MRI) can be used noninvasively to monitor nerve degeneration and regeneration. The aim of this study was to investigate nerve repair after MSC transplantation combined with microenvironment immunomodulation in neurotmesis by using multiparametric MRI.Methods: Rats with sciatic nerve transection and surgical coaptation were treated with MSCs combined with immunomodulation or MSCs alone. Serial multiparametric MRI examinations were performed over an 8-week period after surgery.Results: Nerves treated with MSCs combined with immunomodulation showed better functional recovery, rapid recovery of nerve T2, fractional anisotropy and radial diffusivity values, and more rapid restoration of the fiber tracks than nerves treated with MSCs alone.Discussion: Transplantation of MSCs in combination with immunomodulation can exert a synergistic repair effect on neurotmesis, which can be monitored by multiparametric MRI. K E Y W O R D Simmunomodulation, magnetic resonance imaging, mesenchymal stem cells, peripheral nerve injuries, tacrolimus, toll-like receptors Abbreviations: AD, axial diffusivity; BDNF, brain-derived neurotrophic factor; DTI, diffusion tensor imaging; DTT, diffusion tensor tractography; ELISA, enzyme-linked immunosorbent assay; FA, fractional anisotropy; FKBP, FK-binding protein; GDNF, glial cell-derived neurotrophic factor; GFP, green fluorescent protein; LPS, lipopolysaccharide; MD, mean diffusivity; MRI, magnetic resonance imaging; MSC, mesenchymal stem cell; PBS, phosphate-buffered saline; RD, radial diffusivity; SD, Sprague-Dawley; SFI, sciatic nerve function index; SPRR1A, small proline-rich protein 1A; TLR4, toll-like receptor 4; TNF-α, tumor necrosis factor-α.Zehong Yang and Chushan Zheng contributed equally to this work.

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Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury

Cited by 22 publications

References 41 publications

SPP1 Promotes Schwann Cell Proliferation and Survival through PKCα by Binding with CD44 and αvβ3 after Peripheral Nerve Injury

SPP1 Promotes Schwann Cell Proliferation and Survival through PKCα by Binding with CD44 and αvβ3 after Peripheral Nerve Injury

Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury

Magnetic resonance imaging of enhanced nerve repair with mesenchymal stem cells combined with microenvironment immunomodulation in neurotmesis

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