Effect of stabilizers on the shelf-life of Penicillium frequentans conidia and their efficacy as a biological agent against peach brown rot

Guijarro, Belén; Melgarejo, P.; Cal, A. De

doi:10.1016/j.ijfoodmicro.2006.06.024

Cited by 53 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This half-life figure is close to that obtained by Batta (2004) with T. harzianum conidia formulated in reverse emulsion (21 weeks). Several studies have found that formulated conidia remain viable for longer periods than non-formulated conidia (Guijarro et al, 2007;Larena et al, 2007;Wijesinghe et al, 2010Wijesinghe et al, , 2011. This prolonged viability is thought to be related to the specific role of the various ingredients of the formulation -in this case glucose and soybean oil.…”

Section: Discussionmentioning

confidence: 99%

“…This prolonged viability is thought to be related to the specific role of the various ingredients of the formulation -in this case glucose and soybean oil. The contribution of glucose to increasing the viability of microbial propagules was documented by Guijarro et al (2007), who showed that amending a formulation with a source of carbon such as 7.5% glucose made it possible to keep Penicillium frequentans conidia alive for 12 months. Moreover, oils may also help to boost conidial viability by providing the microorganisms with a food basis while regulating water availability (Paau, 1998).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

et al. 2014

View full text Add to dashboard Cite

HIGHLIGHTS• New oil-based dispersion with a half-life of T. asperellum conidia of 22.5 weeks.• Complete inhibition of P. megakarya on sprayed detached pods.• Enhanced rate and duration of protection on sprayed cacao pods in the field.• 50% of pods protected 3.2 weeks after spraying in the field.• Formulation suitable for the spraying equipment of small-scale cacao producers. GRAPHICAL ABSTRACTFormulated conidia of Trichoderma provide similar or even better protection of cacao pods against black pod disease than a conventional fungicide. ABSTRACTIn African cacao-producing countries, control of cacao black pod disease caused by Phytophthora megakarya is a priority. Introducing biological control agents as part of a P. megakarya control strategy is highly desirable, especially in a perspective of pesticide reduction. Trichoderma species are among the most used biological control agents. In Cameroon, Trichoderma asperellum formulated in wettable powder has produced positive effects against this disease. However, with this type of formulation, shelf-life and persistence of conidia on pods are limited. Our study therefore sought to develop a new T. asperellum formulation that would be more effective and better suited to the conditions of field application by small-scale producers in Cameroon. We selected a soybean oil-based oil dispersion, in which the half-life of the conidia reached 22.5 weeks, versus 5 weeks in Published in : Biological Control (2014), vol. 77, pp. 15-22 Status : Postprint (Author's version) aqueous suspension. Tested on detached pods, the formulation completely inhibited the development of the disease. When sprayed in the field on cacao clones highly sensitive to P. megakarya, the formulation resulted in 90% protection of treated pods after 1 week, and 50% after 3.2 weeks. The formulations exercised a measurable effect for up to 7 weeks, versus 2 weeks in the case of an aqueous conidial suspension and 5 weeks for that of a conventional fungicide (Kocide). Trichoderma asperellum formulated in oil dispersion has therefore great potential for the control of cacao black pod disease with less recourse to synthetic fungicides.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Sucrose solution is an osmotic protector (Burges 1998) leading to preserving of spore survival and germination (Jackson and Erhan 2006;Shabana et al 2003). Sodium glutamate as a stabilizer and osmotic protector (Guijarro et al 2007;Larena et al 2007) increased shelf life of fungal spores during drying period (Burges 1998). Sodium alginate prevented loss of viability of chlamydospores during Pasta production and improved the mycoherbicidal efficacy, stability, and shelf-life comparing to the standard Pasta with no additives (Shabana et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…This feature prevents the suspension formation in water (Behle et al 2006). Some research showed that the wettable powder formulation from fungal conidia by using wetting agent might solve this problem (Guijarro et al 2007;Nagayama et al 2007). Many of the currently available biological pesticide products are wettable powder containing conidia (Burges 1998).…”

Section: Introductionmentioning

confidence: 99%

Effect of Formulating of Beauveria Bassiana Conidia on their Viability and Pathogenicity to the Onion Thrips, Thrips Tabaci Lind. (Thysanoptera: Thripidae)

Ezzati-Tabrizi¹,

Talaei-Hassanloui²,

Pourian³

2009

Journal of Plant Protection Research

View full text Add to dashboard Cite

Abstract:The wettable powder was prepared on the basis of aerial conidia of two isolates of the entomopathognic fungus, Beauveria bassiana. Viability and pathogenicity of conidia products were evaluated against the second-instar larva of Thrips tabaci in four cases;Conidial-product Maintained in Refrigerator (CMR), Conidial-product Maintained in Laboratory (CML), New Formulated Conidia (NFC) and New Conidia without formulation (NC). Analysis of corrected seven-day total mortality data revealed that there were significant differences among these product-cases in their pathogenicity to thrips larvae. Recorded mortality rates for CMR, CML, NFC and NC showed that the pathogenicity of CML was lower compared to three other cases for both isolates. In the next step, inorganic salts (MgCl 2 , NH 4 PO 4 , KH 2 PO 4 , MgSO 4 and NaCl) were added at a rate of 0.1 M into the both CMR and CML products. Bioassay results indicated that caused total mortality of thrips larvae increased with adding of salts. Our results showed that applied carriers and salts have positively effected preserving of conidia viability and pathogenicity to the second-instar larva of the onion thrips.

show abstract

“…It has shown a good potential for development as a commercial biocontrol product against the brown rot of peach fruit (Guijarro et al 2006(Guijarro et al , 2007. It produced high levels of extracellular pectinases after 24 h of incubation in submerged culture (Kawano et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of certain Penicillium frequentans isolates against Cercospora leaf spot disease of sugar beet

El-Fawy

El-Sharkawy

Abo-Elyousr

2018

Egypt J Biol Pest Control

View full text Add to dashboard Cite

The impact of six local isolates of Penicillium frequentans recovered from healthy sugar beet (Beta vulgaris L.) leaves was evaluated against Cercospora beticola, the causal pathogen of Cercospora sugar beet leaf spot under laboratory and field conditions. In in vitro studies, all the six isolates were able to inhibit the mycelial growth of C. beticola with variation in their antagonistic capability. P. frequentans isolates produce pectinase and cellulase at different degrees. There was a correlation between enzyme activity and the antagonistic ability for each isolate. The high antagonistic ability isolates had the most enzyme activity. In field studies, some adhesives such as agar, starch flour, white glue, gum, and commercial adhesive (Triton Mok) were added to conidia spore suspensions of P. frequentans at 1% to improve conidial adhesion to sugar beet plant surface. Data also showed that all adhesives increased (P = 0.05) the efficiency of the spore suspension of P. frequentans to control the disease. The starch flour at 1% gave a significant reduction in disease severity from 43.23 to 10.42% pre-infection and from 43.23 to 10.52% post-infection. The application of P. frequentans led to improved root yield and the sugar percent of sugar beet crop in two tested seasons.

show abstract

Effect of stabilizers on the shelf-life of Penicillium frequentans conidia and their efficacy as a biological agent against peach brown rot

Cited by 53 publications

References 18 publications

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

Effect of Formulating of Beauveria Bassiana Conidia on their Viability and Pathogenicity to the Onion Thrips, Thrips Tabaci Lind. (Thysanoptera: Thripidae)

Evaluation of certain Penicillium frequentans isolates against Cercospora leaf spot disease of sugar beet

Contact Info

Product

Resources

About