Effect of Staphylococcus Aureus infection on plasma creatine kinase activity in rabbits

Abstract

The aim of the present study was to investigate the variations of the plasma creatine kinase (CK) activities following an experimentally induced staphylococcal infection in rabbits and to compare them with the occurrence of clinical signs. For that, the plasma CK activities were determined in 7 male New Zealand White, 3 months old rabbits subcutaneously injected with Staphylococcus aureus strain suspension (density: 8х10 8 cfu/mL) and in 6 other rabbits not treated (controls). In parallel, rectal temperature and skin lesions (abscesses) were recorded. In all infected animals, formation of abscess due to the proliferation of the inoculated strain was observed within 48-96 hours following the bacterial inoculation and these lesions have gradually extended leading to purulent exudates several days after and sometimes to secondary abscesses in surrounding muscles. The concentration of fibrinogen was used as an acute phase protein with aim to confirm the incidence of infection. Plasma CK activities were dramatically elevated on 48 hour compared to initial values and to controls at the same time but this increase was transient, the CK activities being similar to controls since the 72 nd hour. These results indicate that the plasma CK activity in rabbit can be considered as a precocious marker of skin and secondary muscular damage related to the abscess formation and probably to the bacterial exotoxins following experimental staphylococcal infection.

Pathological Studies on Infertility in Bucks as a Sign to Some Bacterial Infection

“…Rabbits of group (3) sacrificed at 35 days PI showed congestion of upper respiratory tract without discharge, subcutaneous small abscesses were noticeable, no marked changes among rabbits of group (5) while at 45 days PI two rabbits out of six of group (3) showed spreading diffuse subcutaneous swelling (phlegmon) Fig. (C) that mainly affected the dorsal posterior region and appeared as white gray diffuse caseated matter which not appeared in rabbits of group (5) this sign was similar to that described by Georgieva et al (2016) and could be attributed to the limited extent and activity of Staph. aureus causing acute suppurative inflammation (phlegmon) (Lowy, 1998).…”

“…While rabbits of group (5) showed significant decrease in comparison with those of group (1) and rabbits of groups (2, 3 and 4) showed highly significant decrease in germinal epithelium highest when compared with those of group (1) at 45 days PI (Table 14). These results were matching with our microscopical results of testis and at the same time confirm those obtained by Johnson score and semin analysis on a base of orchitis and epididemitis induced by both previously challenged isolates (James et al, 2002;Megan et al, 2015;Georgieva et al ., 2016). -------------------------6 Group( 1…”

“…aureus showed marked differences than control and treated groups. Marked decrease of sexual activities, behavior and desire among rabbits of groups (2 and 3), while it's less noticeable in groups (4 and 5), gradual rise of body temperature within first two weeks PI among rabbits of groups (2 and 3) that was in complete accordance with that mentioned by Georgieva et al (2016). Respiratory manifestations (sneezing with catarrhal to purulent discharge as a thick yellowish gray matter) among rabbits of group (2) were noticeable.…”

“…The rabbits were inoculated S/C with 0.1 ml of inoculum having 8×10 8 colony forming units (CFUs)/ ml and with normal saline in control group as done by Georgieva et al (2016).…”

“…Staphylococcus aureus is an adaptive opportunistic pathogen, capable to persist and replicate under various conditions. In rabbits, problems of staphylococcosis arise when S. aureus bacteria infect small dermal lesions and invade subcutaneous tissue (Georgieva et al, 2016), provoking a number of lesions including visceral abscesses, pod dermatitis, and mastitis. Occasionally, abscesses in internal organs, most commonly in liver and lungs were observed (Bisnoa and Stevens, 1996).…”